SYNTHESIS OF SILVER NANOPARTICLES FROM THE MEDICINAL PLANT BAUHINIA ACUMINATA AND BIOPHYTUM SENSITIVUM–A COMPARATIVE STUDY OF ITS BIOLOGICAL ACTIVITIES WITH PLANT EXTRACT
DOI:
https://doi.org/10.22159/ijap.2017v9i1.16277Keywords:
Silver nanoparticle synthesis, Bauhinia acuminata, Biophytum sensitivum, FTIR, SEM, Antimicrobial activityAbstract
Objective: The aim of current study was to synthesise silver nanoparticles from the leaf extracts (aqueous and methanol) of two medicinal plants Bauhinia acuminata and Biophytum sensitivum and to compare its biological activities with that of plant extract.
Methods: Silver nanoparticles were synthesised, and it was characterised using UV-Visible spectroscopy and scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis were done for silver nanoparticle extract. The biological activities such as DPPH scavenging assay, haemolytic assay and antimicrobial assay were done for both nanoparticle and plant extract.
Results: The UV-Visible spectroscopy showed the formation of nanoparticles in a size range of 400-460 nm. GC-MS analysis showed the presence of biologically active compounds like DL-alpha-tocopherol and Alpha-tocopherol-beta-D-mannose. FTIR analysis of silver nanoparticles and leaf extracts showed the formation of aldehydes, alkenes, amines, alcohols, etc., which confirmed the presence of the compounds present in plant extracts. SEM image showed the formation of nanoparticles of size 2 micrometre. Phytochemical analysis of plant extracts showed the presence of carbohydrates, phenols, flavonoids, saponins, tannins and terpenoids. The methanol extract of Bauhinia acuminata showed high DPPH scavenging activity of 90% compared to that of the silver nanoparticle. The percentage hemolysis of all extracts was found to be 6%-39%. The antimicrobial activity of leaf extracts showed excellent activity towards Bacillus cereus and Listeria monocytogens.
Conclusion: The results of present study showed that the silver nanoparticle synthesised from the plant extract has many bioactive compounds and it was found to have significant biological activities but comparatively lesser than plant extract. It concludes the both plant and nanoparticle extract can be used as a potential resource for therapeutic purpose.
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Akhila Sravya Dantu, Shankarguru P, Ramya devi D, Vedha Hari Bn. Evaluation of in vitro anticancer activity of hydroalcoholic extract of Tabernaemontana divaricate. Asian J Pharm Clin Res 2012;5:59-61.
Ashokkumar S, Ravi S, Kathiravan V, Velmurugan S. Synthesis of silver nanoparticles using A. indicum leaf extract and their antibacterial activity. Spectrochim Acta Part A 2015;134:34-9.
Kanchana R, Pranjita Zantye. Plant-mediated synthesis of silver nanoparticles with diverse applications. Asian J Pharm Clin Res 2016;9:159-63.
Khandelwal N, Kaur G, Kumara N, Tiwari A. Application of silver nanoparticles in viral inhibition: a new hope for antivirals. Dig J Nanomater Biostruct 2014;9:175.
Park S, Park HH, Kim SY, Kim SJ, Woo K, Ko G. Antiviral properties of silver nanoparticles on a magnetic hybrid colloid. Appl Environ Microbiol 2014;80:2343-50.
Anju Krishna SR, Hafza S, Poorna Chandrika G, Lekhya Priya C, Bhaskara Rao KV. Pharmacological properties, phytochemical and GC-MS analysis of Bauhinia acuminata Linn. J Chem Pharm Res 2015;7:372-80.
Arokiya FAA, Arumugam R, Saravanan S, Anantharaman P. Photofabrication of gold nanoparticles assisted by leaves of Suaedamonica and its free radical scavenging property. J Photochem Photobiol B: Biol 2014;135:75-80.
Pannerselvam, Balashanmugam, Pudupalayam T, Kalaichelvan. Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC aqueous extract and coated on cotton cloth for effective antibacterial activity. Int J Nanomed 2015;10:87-97.
Harbone JB. Phytochemical methods, a guide to modern techniques of plant analysis. Chapman and Hall, London; 1973.
Blios MS. Antioxidant determination by the use of a stable free radical. Nature 1958;29:1199-200.
Priya CL, Gaurav K, Bhaskara RKV. Antioxidant activity of Achyranthesaspera Linn stems extracts. Pharmacologyonline 2010;2:228-37.
Krishnaraj C, Jagan EC, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N. Synthesis of silver nanoparticles using Acalyphaindica leaf extract and its antibacterial activity against water borne pathogens. Collid Surf B: Biointer 2010;76:50-6.
Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2175-84.
Anjukrishna SR, Hafza S, Poorna CG, Lekhya PC, Bhaskara RKV. Pharmacological properties, phytochemical and GC-MS analysis of Bauhinia acuminata Linn. J. Chem Pharm Res 2015;7:372-80.
Anjukrishna SR, Hafza S, Poorna CG, Lekhya PC, Bhaskara RKV. Pharmacological properties, phytochemical and GC-MS analysis of Bauhinia acuminata Linn. J Chem Pharm Res 2015;7:372-80.
Venkata NK, Tadi R, Sudhakara RP, Sandeep BV. Assessment of phytoconstituents composition and anti-haemolytic activity of Psidiumguajava leaf extract against H2O2 induced hemolysis in chicken erythrocytes. Int J Pharm Sci Res 2016;1:27-30.
Charalampos P, Michael. Analysis of naturally occurring phenolic compounds in aromatic plants by RP-HPLC coupled to diode array detector (DAD) and GC-MS after Silylation, Foods. Foods 2013;2:90-9.
Anjukrishna SR, Hafza S, Poorna CG, Lekhya PC, Bhaskara RKV. Pharmacological properties, phytochemical and GC-MS analysis of Bauhinia acuminata Linn. J Chem Pharm Res 2015;7:372-80.
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