GREEN SYNTHESIS OF SILVER NANOPARTICLES USING MEDICINAL PLANT ACALYPHA INDICA LEAF EXTRACTS AND ITS APPLICATION AS AN ANTIOXIDANT AND ANTIMICROBIAL AGENT AGAINST FOODBORNE PATHOGENS
DOI:
https://doi.org/10.22159/ijap.2017v9i5.19464Keywords:
Green synthesis, Silver nanoparticles, DPPH activity, Reducing power, Antifungal, and food preservationAbstract
Objective: In the present study, silver (Ag) nanoparticles was synthesized by traditionally used medicinal plant Acalypha indica, which was characterized using various advanced tools, and its antioxidant as well as antimicrobial properties, was studied against food pathogens.
Methods: The synthesis of silver (Ag) nanoparticles from the leaf extracts were monitored with the characterization of silver nanoparticles with the help of UV-visible spectrophotometer. The optimized time for the synthesis of nanoparticles was 3 h, followed by fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), surface emission microscopy analysis (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM) and energy dispersive spectroscopy analysis (EDX). The antioxidant activity was evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay, while antifungal activity was performed against food pathogens by measuring the zone of inhibition values (ZOI).
Results: The Ag nanoparticle produced, have an average particle size of 34 nm with a spherical shape, analyzed from the XRD studies, and size was confirmed with the SEM and TEM analysis. The FTIR analysis gave information about the possible compounds adsorbed on the surface of the Ag nanoparticles. The Ag nanoparticles had good reducing power than the standard and the IC50 value for Ag nanoparticles was 5 mg/ml, while the standard taken had an IC50 value of nearly 6-7 mg/ml. The fungal strain A. fumigates showed ZOI of 133% at 75 µl of concentration proving that Ag nanoparticles can act effectively against this strain when compared to other strains even at low concentrations.
Conclusion: The produced Ag nanoparticles can be used for its therapeutic purposes and for large-scale synthesis in food industries for food preservation or packaging.
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Krithiga N, Rajalakshmi A, Jayachitra A. Green synthesis of silver nanoparticles using leaf extracts of Clitoria ternatea and Solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. Int J Nanosci 2015;8. http://dx.doi.org/10.1155/2015/928204
Rajeshkumar S, Malarkodi C, Vanaja M, Annadurai G. Anticancer and enhanced antimicrobial activity of biosynthesized silver nanoparticles against clinical pathogens. J Pharm Technol 2016;1116:165.
Gavhane A, Padmanabhan P, Kamble S, Jangle S. Synthesis of silver nanoparticles using extract of neem leaf and triphala and evaluation of their antimicrobial activities. Int J Pharma Bio Sci 2012;3:88.
Bobbu P, Netala VR, Aishwarya S, Reddy IRM, Kotakadi VS, Tartte V. Rapid synthesis of silver nanoparticles using aqueous leaf extract of Achyranthes aspera and study of their antimicrobial and free radical scavenging activities. Int J Pharm Pharm Sci 2016;8:341-6.
Begum NA, Mondal S, Basu S, Laskar RA, Mandal D. Biogenic synthesis of Au and ag nanoparticles using aqueous solutions of Black Tea leaf extracts. ‎Colloids Surf B 2009;71:113.
Sujitha MV, Kannan S. Green synthesis of gold nanoparticles using citrus fruits (Citrus limon, Citrus reticulata and Citrus sinensis) aqueous extract and its characterization. Spectrochim Acta A Mol Biomol Spectrosc 2013;102:15.
Longhi JG, Perez E, Lima JJ, Candido LMB. In vitro evaluation of Mucuna pruriens (L.) Dc. Antioxidant activity. Braz J Pharm Sci 2011;47. http://dx.doi.org/10.1590/S1984-82502011000300011
Das RK, Gogoi N, Bora U. Green synthesis of gold nanoparticles using Nyctanthes arbortristis flower extract. Bioprocess Biosyst Eng 2011;34:615.
Christensen L, Vivekanandhan S, Misra M, Mohanty AK. Biosynthesis of silver nanoparticles using Murraya koenigii (curry leaf): an investigation on the effect of broth concentration in reduction mechanism and particle size. Adv Mater Lett 2011;2:429.
Ali ZA, Yahya R, Sekaran SD, Puteh R. Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Adv Mater Sci Eng 2016;6. http://dx.doi.org/10.1155/2016/4102196
Rajaselvam J, Benila Smily JM, Meena R. A study of antimicrobial activity of Acalypha indica against selected microbial species. Int J Life Sci Pharma Res 2012;3:473-6.
Somchit MN, Abdul Rashid R, Abdullah A, Zuraini A, Zakaria ZA, Sulaiman MR, et al. In vitro antimicrobial activity of leaves of Acalypha indica linn (Euphorbiaceae). Afr J Microbiol Res 2010;4:2133.
Wollinger A, Perrin E, Chahboun J, Jeannot V, Touraud D, Kunz W. Antioxidant activity of hydro distillation water residues from Rosmarinus officinalis leaves determined by DPPH assays. C R Chim 2016;19:754.
Patel RM, Patel NJ. In vitro antioxidant activity of coumarin compounds by DPPH, super oxide and nitric oxide free radical scavenging methods. J Adv Pharm Technol Res 2011;1:52.
Jayanthi P, Lalitha P. Reducing the power of the solvent extracts of Eichhornia crassipes (mart.) solms. Int J Pharm 2011;3:126-8.
Rajeshkumar S, Malarkodi C, Paulkumar K, Vanaja M, Gnanajobitha G, Annadurai G. Algae mediated green fabrication of silver nanoparticles and examination of its antifungal activity against clinical pathogens. Int J Met 2014;1. http://dx.doi.org/10.1155/2014/692643
Kumar G, Karthik L, Rao KVB. Antibacterial activity of aqueous extract of Calotropis gigantea leaves, an in vitro study. Int J Pharm Sci Rev Res 2010;4:141.
Viveka R, Thangama R, Muthuchelian K, Gunasekaran P, Kaveri K, Kannana S. Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochem 2012;47:2405.
Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar S, Kalaichelvan PT, Mohan N. Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B 2010;76:50.
Bykkam S, Ahmadipour M, Narisngam S, Venkateswara, Kalagadda R, Chidurala SC. Extensive studies on X-ray diffraction of green synthesized silver nanoparticles. Adv Nanopart 2015;4:1-10.
Phatak RS, Hendre AS. Sunlight induced green synthesis of silver nanoparticles using sundried leaves extract of Kalanchoe pinnata and evaluation of its photocatalytic potential. Der Pharm Lett 2015;7:313.
Joy PH, Johnson I. Plant-mediated biosynthesis and characterization of silver nanoparticles by leaf extracts of Tragia involucrata, Cymbopogon citronella, Solanum verbascifolium and Tylophora ovata. Karbala Int J Mod Sci 2015;1:237.
Saware K, Sawle B, Salimath B, Jayanthi K, Venkataraman A. Biosynthesis and characterization of silver nanoparticles using Ficus Benghalensis leaf extract. Int J Res Eng Technol 2014;3:867.
Bhuiyan MAR, Hoque MZ, Hossain SJ. Free radical scavenging activities of Zizyphus mauritiana. World J Agric Sci 2009;5:318.
Moein MR, Moein S, Ahmadizadeh S. Radical scavenging and reducing power of Salvia mirzayanii subfractions. Molecules 2008;13:2804.
Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, et al. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B 2010;81:81.
Asha S, Asha A, Rajeshkumar S. Evaluation of phytochemical constituents and antimicrobial activity of silver nanoparticle synthesized Ipomoea nil against selected pathogens. Asian J Pharm Clin Res 2017;10:1-5.
Rajeshkumar S, Malarkodi C, Venkat KS. Synthesis and characterization of silver nanoparticles from marine brown seaweed and its antifungal efficiency against clinical fungal pathogens. Asian J Pharm Clin Res 2017;10:190-3.
Wilson A, Prabukumar S, Sathishkumar G, Sivaramakrishnan S. Aspergillus flavus mediated silver nanoparticles synthesis and evaluation of its antimicrobial activity against different human pathogens. Int J Appl Pharm 2016;8:43-6.
Murugesan S, Bhuvaneswari S, Sivamurugan V. Green synthesis, characterization of silver nanoparticles of a marine red alga Spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;9:192-7.
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