THE EFFECT OF CURCUMA XANTHORRHIZA - ETHANOL EXTRACT TO BIOFILM FORMATION OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)
DOI:
https://doi.org/10.22159/ijap.2017.v9s1.14_16Keywords:
Biofilm formation, Single-species biofilm, Dual-species biofilm, Streptococcus mutans, Aggregatibacter actinomycetemcomitans, Curcuma xanthorrhiza ethanol extract, Crystal violet assayAbstract
Objective: This in vitro study aimed to analyze the mass ratio of single- and dual-species Streptococcus mutans and Aggregatibacter
actinomycetemcomitans biofilm after exposure to Curcuma xanthorrhiza ethanol extract (Xan).
Methods: A bacterial suspension in brain heart infusion medium, enriched with 0.2% sucrose, was exposed to the Xan, incubated for 18 hrs, and
analyzed using a crystal violet assay.
Results: This research concluded that the minimum inhibitory concentration of ethanol-temulawak extract against S. mutans was 5%, while the
minimum bactericidal concentration was 15%.
Conclusions: Xan prevented biofilm formation of single-species S. mutans and dual-species S. mutans and A. actinomycetemcomitans more effectively
than it did single-species A. actinomycetemcomitans.
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