TRANSFERSOME GEL FORMULATION OF AN ETHANOL EXTRACT OF APPLES (MALUS DOMESTICA MILL) CONTAINING ANTIOXIDANTS AND IN VITRO PENETRATION TESTING USING FRANZ DIFFUSION CELLS

Authors

  • Nurarita Fadila Zesiorani Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
  • Effionora Anwar Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2017.v9s1.19_24

Keywords:

Apple extract gel, Transfersome, Formulation

Abstract

Objective: This study aims to formulate and characterize a transfersome apple peel extract, formulate it into a gel, and compare it with a control gel
made without transfersome.
Methods: Both gels were evaluated, stability tested, and penetration tested using Franz diffusion cells on the skin of female Sprague-Dawley rats. The
transfersome preparations were formulated with different concentrations of the active substance, quercetin: 0.5% (F1); 0.7% (F2), and 1.0% (F3).
Results: Based on the characterization results, F1 was selected as the optimum gel formulation because it had spherical morphology, a Dmean volume of
106.44±2.70 nm, a polydispersity index of 0.078±0.01, a zeta potential of −49.96±2.05 mV, and a drug efficiency entrapment percentage of 78.78±0.46%.
The cumulative amount of quercetin that was penetrated with the transfersome gel was 1514.41±26.31 μg/cm2, whereas the penetration with the
control gel extract was 1133.62±18.96 μg/cm2. The cumulative percentages of the penetrated gel transfersome and gel extract were 78.40±1.89%
and 49.89±0.88%, respectively. The fluxes of transfersome gel and control gel extract were 52.33±0.11 μg/cm²/hrs and 40.89±0.68 μg/cm²/hrs,
respectively.
Conclusions: Based on these results, it can be concluded that the gel with transfersome exhibited better penetration than the gel extract alone.

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References

Stenvinkel P, Larsson TE. Chronic kidney disease: A clinical model of

premature aging. Am J Kidney Dis 2013;62(2):339-51.

Sen S, Chakraborty R, Sridhar C, Reddy YS, De B. Free radicals,

antioxidants, diseases and phytomedicines: Current status and future

prospect. Int J Pharm Sci Rev Res 2010;3(1):91-100.

Moore RS, Taylor DH, Reddy MM, Sturman LS, Sarma AD,

Mallick AR, et al. Free radical and their role in different clinical

conditions; An overview. Int J Pharm Sci Res 2010;1(3):185-92.

Benson HA. Transdermal drug delivery: Penetration enhancement

techniques. Curr Drug Deliv 2005;2(1):23-3.

Widyanati P, Jufri M, Elya B. Formulation and penetration dtudy of

liposome gel xanthone of extract mangosteen Pericarp (Garcinia

mangostana L.). Int J Pharm Sci 2014;27(2):1-6.

Taufikkurohmah T. Sintesis p-metoksisinamil dari etil p-metoksisinamat

hasil isolasi Rimpang kencur (Kaempferia galanga L.) sebagai

Kandidat Tabir Surya. Indones J Chem 2005;5(3):193-7.

Deore S, Baviskar B, Khadabadi S, Nikole K. Isolation and quantitative

estimation of quercetin in Lagenaria siceraria fruit. J Chromatogr Sep

Tech 2013;4(6):2-4.

Boyer J, Liu RH. Apple phytochemicals and their health benefits. Nutr

J 2004;3:5.

Costa S, Detoni C, Branco C, Botura M, Branco A. In vitro

photoprotective effects of Marcetia taxifolia ethanolic extract and

its potential for sunscreen formulations. Rev Bras Farmacogn

;25(4):413-8.

Saewan N, Jimtaisong A. Photoprotection of natural flavonoids. J Appl

Pharm Sci 2013;3(9):129-41.

Kelly GS. Quercetin. Monograph. Altern Med Rev 2011;16(2):172-94.

Lipowsky R, Sackmann E. Structure and Dynamics of Membranes.

st ed. North Holland: Elsevier; 1995.

Benson HA. Transfersomes for transdermal drug delivery. Expert Opin

Drug Deliv 2006;3(6):727-37.

Indonesian Ministry of Health. Farmakope Indonesia Edisi III. Jakarta:

Korpri Sub Unit Direktorat Jenderal Pengawasan Obat dan Makanan; 1979.

Windono TJ, Soeratri W. Aktivitas tabir matahari etil p-metoksisinamat

yang diisolasi dari rimpang kencur (Kaempferia galanga L.). News of

Indones Drug 1997;38:430-1.

Published

30-10-2017

How to Cite

Zesiorani, N. F., & Anwar, E. (2017). TRANSFERSOME GEL FORMULATION OF AN ETHANOL EXTRACT OF APPLES (MALUS DOMESTICA MILL) CONTAINING ANTIOXIDANTS AND IN VITRO PENETRATION TESTING USING FRANZ DIFFUSION CELLS. International Journal of Applied Pharmaceutics, 9, 32–37. https://doi.org/10.22159/ijap.2017.v9s1.19_24

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