SALIVARY ALPHA AMYLASE LEVELS DURING PRIMARY TOOTH EXTRACTION IN CHILDREN AGED 6â€“11 YEARS
Keywords:Salivary alpha amylase levels, Anxiety, Tooth extraction, Nil
Objective: Patients generally experience some level of anxiety during dental treatments. Salivary alpha amylase (SAA) can be used as a biomarker for anxiety. This study aimed to compare SAA levels between children who had never and had undergone tooth extraction procedures with a local anesthetic injection.
Methods: Thirty-seven participants aged 6â€“11Â years who were to undergo the extraction of primary teeth at the Dental and Oral Educational Hospital, Faculty of Dentistry Universitas Indonesia, were enrolled. The subjects were divided into two groups: One group consists of 20 children who had never undergone a tooth extraction, and the other group consists of 17 children who had undergone tooth extraction. From all children, one saliva sample was collected using a portable saliva strip 10Â min before (t0), shortly after (t1), and 10Â min after (t2) local anesthetic injection, and the SAA activity was determined using a portable Nipro Cocoro Meter device. The SAA levels of both groups were analyzed using the Mann-Whitney U test.
Results: There were significant differences in SAA levels at t0 (p=0.001), t1 (p=0.018), and t2 (p=0.021) in both groups. Anxiety is a combination of behavioral and physiological reactions. SAA release is regulated by autonomic innervation, and the SAA level increases owing to acute stress.Conclusion: Dentists should note patients' negative dental experiences to provide more effective and less traumatic treatment.
Koh D, Ng V, Naing L. Alpha amylase as a salivary biomarker of acute stress of venepuncture from periodic medical examinations. Front Public Health 2014;2:121.
Oosterink FM, de Jongh A, Aartman IH. What are people afraid of during dental treatment? Anxiety-provoking capacity of 67 stimuli characteristic of the dental setting. Eur J Oral Sci 2008;116:44-51.
Strahler J, Mueller A, Rosenloecher F, Kirschbaum C, Rohleder N. Salivary alpha-amylase stress reactivity across different age groups. Psychophysiology 2010;47:587-95.
Yamaguchi M, Kanemori T, Kanemaru M, Takai N, Mizuno Y, Yoshida H, et al. Performance evaluation of salivary amylase activity monitor. Biosens Bioelectron 2004;20:491-7.
Higashi T, Mizuno Y, Yamaguchi M. Development of Sympathetic Nervous System Activity Monitor Using Salivary Amylase Activity. Technical Papers and Articles; 2005.
Bosch JA, Brand HS, Ligtenberg TJ, Bermond B, Hoogstraten J, Nieuw Amerongen AV. Psychological stress as a determinant of protein levels and salivary-induced aggregation of no impact of saliva flow rate on amylase stress response 651 streptococcus gordonii in human whole saliva. Psychosom Med 1996;58:374-82.
Engert V, Vogel S, Efanov SI, Duchesne A, Corbo V, Ali N, et al. Investigation into the cross-correlation of salivary cortisol and alpha-amylase responses to psychological stress. Psychoneuroendocrinology 2011;36:1294-302.
de Menezes Abreu DM, Leal SC, Mulder J, Frencken JE. Patterns of dental anxiety in children after sequential dental visits. Eur Arch Paediatr Dent 2011;12:298-302.
Goettems ML, Ardenghi TM, Romano AR, Demarco FF, Torriani DD. Influence of maternal dental anxiety on the childâ€™s dental caries experience. Caries Res 2012;46:3-8.
Hmud R, Walsh L. Dental anxiety: Causes, complications and management approaches. J Minim Interv Dent 2009;2:67-78.