• Hamzeh Alkhatib Advanced Drug Delivery Lab, Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia
  • Farahidah Mohamed IKOP Sdn Bhd, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200 Kuantan, Malaysia
  • Abd Almonem Doolaanea IKOP Sdn Bhd, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200 Kuantan, Malaysia



Black seed oil, Nigella sativa, ATR-FTIR, Encapsulation, Alginate, turbidity


Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement.

Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer.

Results: ATR-FTIR method exhibited linearity in the range of 25-300 mgBSO/mlemulsion (mgBSO/mlE)with R2=0.998, RSD=8.4%, LOD=0.28 mgBSO/mlE and LOQ=0.87 mgBSO/mlE. BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mgBSO/mlE, R2=0.9931, RSD=1.34%, LOD=0.89 mgBSO/mlE, and LOQ=2.71 mgBSO/mlE. In addition, the method showed that total amount of BSO was released at 110 min.

Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology.


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Ali B, G Blunden. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 2003;17:299-305.

Sahak MKA, N Kabir, G Abbas, S Draman, NH Hashim, DS Hasan Adli. The role of Nigella sativa and its active constituents in learning and memory. J Evidence Based Complementary Altern Med 2016.

Gholamnezhad Z, R Keyhanmanesh, MH Boskabady. Anti-inflammatory, antioxidant, and immunomodulatory aspects of Nigella sativa for its preventive and bronchodilatory effects on obstructive respiratory diseases: a review of the basic and clinical evidence. J Funct Foods 2015;17:910-27.

Srinivasan K, Cumin (Cuminum cyminum) and black cumin (Nigella sativa) seeds: traditional uses, chemical constituents, and nutraceutical effects. FQS 2018;2:1-16.

Tavakkoli A, V Mahdian, BM Razavi, H Hosseinzadeh. Review on clinical trials of black seed (Nigella sativa) and its active constituent, thymoquinone. J Pharmacopuncture 2017;20:107-11.

Tønnesen HH, J Karlsen. Alginate in drug delivery systems. Drug Dev Ind Pharm 2002;28:621-30.

Suganya V, S Asheeba. Microencapsulation of astaxanthin using ionotropic gelation method isolated from three crab varieties. Int J Curr Pharm Res 2015;7:96-9.

Hadi MY, GJ Mohammed, IH Hameed. Analysis of bioactive chemical compounds of Nigella sativa using gas chromatography-mass spectrometry. J Pharm Pharmacol 2016;8:8-24.

Bassbasi M, S Platikanov, R Tauler, A Oussama. FTIR-ATR determination of solid non fat (SNF) in raw milk using PLS and SVM chemometric methods. Food Chem 2014;146:250-4.

Vongsvivut J, P Heraud, W Zhang, JA Kralovec, D McNaughton, CJ Barrow. Quantitative determination of fatty acid compositions in micro-encapsulated fish-oil supplements using fourier transform infrared (FTIR) spectroscopy. Food Chem 2012;135:603-9.

Doolaanea AA, FM Ahmad, Fahmi Haruna. Quantification of Nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy. Int J Pharm Pharm Sci 2014;6:228-32.

Doolaanea AA, NI Mansor, NH Mohd Nor, F Mohamed. Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease. J Microencapsul 2016;33:114-26.

Yang H, J Irudayaraj, MM Paradkar. Discriminant analysis of edible oils and fats by FTIR, FT-NIR and FT-Raman spectroscopy. Food Chem 2005;93:25-32.

Reddy KR, M Nagabhushanam. Process and parameters affecting drug release performance of prepared cross-linked alginate hydrogel beads for ezetimibe. Int J Pharm Pharm Sci 2017;9:254-62.

Pearce KN, JE Kinsella. Emulsifying properties of proteins: evaluation of a turbidimetric technique. J Agric Food Chem 1978;26:716-23.

Aoki T, EA Decker, DJ McClements. Influence of environmental stresses on stability of O/W emulsions containing droplets stabilized by multilayered membranes produced by a layer-by-layer electrostatic deposition technique. Food Hydrocoll 2005;19:209-20.

Durante M, MS Lenucci, B Laddomada, G Mita, S Caretto. Effects of sodium alginate bead encapsulation on the storage stability of durum wheat (Triticum durum Desf.) bran oil extracted by supercritical CO2. J Agric Food Chem 2012;60:10689-95.

Reig FB, JG Adelantado, MM Moreno. FTIR quantitative analysis of calcium carbonate (calcite) and silica (quartz) mixtures using the constant ratio method. Application to geological samples. Talanta 2002;58:811-21.

Almeida PF, A Almeida. Cross-linked alginate–gelatine beads: a new matrix for controlled release of pindolol. J Controlled Release 2004;97:431-9.

Guillen M, N Cabo. Characterization of edible oils and lard by fourier transform infrared spectroscopy. Relationships between composition and frequency of concrete bands in the fingerprint region. J Am Oil Chem Soc 1997;74:1281-6.

Zeeb B, AH Saberi, J Weiss, DJ McClements. Retention and release of oil-in-water emulsions from filled hydrogel beads composed of calcium alginate: impact of emulsifier type and pH. Soft Matter 2015;11:2228-36.



How to Cite

Alkhatib, H., Mohamed, F., & Doolaanea, A. A. (2018). ATR-FTIR AND SPECTROSCOPIC METHODS FOR ANALYSIS OF BLACK SEED OIL FROM ALGINATE BEADS. International Journal of Applied Pharmaceutics, 10(5), 147–152.



Original Article(s)