ALOE VERA ETHANOL EXTRACT AS A THERAPY FOR ALVEOLAR MANDIBLE REGENERATION IN LIPOPOLYSACCHARIDE-EXPOSED RATS
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.03Keywords:
Aloe chinensis, Baker ethanol extract, Lipopolysaccharide, Osteoblasts, Osteoclasts, Alveolar boneAbstract
Objective: The study examined the effects of Aloe vera ethanol extract on alveolar mandible regeneration in rats (Rattus norvegicus).
Methods: Rats were divided into five groups as follows: Negative control group received no treatment, positive control group received Escherichia
coli lipopolysaccharide (LPS) alone for 5 days, and Groups P1, P2, and P3 received LPS for 5 days followed by 40, 80, and 160 mg/kg BW A. vera
ethanol extract, respectively, orally on days 6–12. Alveolar bone tissues were taken and histologically processed through hematoxylin-eosin staining.
Osteoblast and osteoclast numbers in alveolar tissue were also examined.
Results: One-way ANOVA revealed strong relationships between the A. vera ethanol extract dosage and the numbers of osteoblasts (r=0.921, p<0.05)
and osteoclasts (r=−0.631, p<0.05) in rats.
Conclusion: A. vera ethanol extract appears capable of stimulating alveolar bone regeneration following LPS exposure.
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References
WHO. The World Oral Health Report. WHO/NMH/ORH/03.2.
Switzerland: World Health Organization; 2003.
Department of Health of Malang. Monthly Report of Community
Health Center 2009. Malang: Department of Health; 2009.
Laine ML, Crielaard W, Loos BG. Genetic susceptibility to periodontitis.
Periodontol 2000 2012;58:37-68.
Carranza FA, Takei HH. Rationale for periodontal treatment. In:
Newman MG, Takei HH, Klokkevold PR, editors. Carranza’s
Clinical Periodontology. 11th ed. Missouri: Saunders Elsevier; 2010.
p. 387-91.
Murray JA, Wilton JM. LPS from periodontal pathogen P. gingivalis
prevents apoptosis of HL60-derived neutrophils in vitro. J Infect
Immun 2003;71:7232-5.
Indahyani DE, Santoso AL, Utoro T, Soesatyo MH. Lypopolisacharide (LPS)
introduction during growth and development period of rat’s tooth towart
the occurance of enamel hypoplasia. Dent J 2007;40:85-8.
Baghban AA, Dehghani A, Ghanavati F. Comparing alveolar bone
regeneration using bio-oss and autogenous bone graft in humas: A
systemic review and meta-analysis. Iran Endod J 2009;4:125-30.
Wiedosari E. Role of natural immunomodulator (Aloe Chinensis in
celullar and humoral immunity system). Wartazoa 2007;17:165-71.
Subramaniam MR, Gauri MU, Shivaraj BW. Bone morphogenetic
proteins: Periodontal regeneration. J Med Sci 2013;5:161-8.
Furnawati I. Use of Aloe Vera. 7th ed. Jakarta: Agro Medika Pustaka; 2006.
Stanshenko P. Interrelationship of Dental Pulp and Apical Periodontitis.
Dental Pulpa. Inc. Chicago: Quintessence Publishing Co.; 2002.
Schwart Z, Goultschin J, Dean DD, Byan BD. Mechanism of alveolar
bone destruction in periodontitis. The pathogenesis of periodontitis.
Periodontology 2000 1997;14:158-72.
Ramamoorthy L, Kemp M.C, Tizard IR. Acemmanan, a beta-(1,4)-
acetylated mannam, induces nitric oxide production in macrophage cell
line RAW 264.7. Am Soc Farmacol Exp Ther 1996;50:878-4.
Saeed MA, Ahmad I, Yaqub U, Akbar S, Waheed A, Saleem M, et al.
Aloe vera: A plant of vital significance. Q Sci Vis 2003;9:1-13.
Selvakumar R, Muralidharan NP. Comparison in benefits of herbal
mouthwashes with chlorhexidine mouthwash: A review. Asian J Pharm
Clin Res 2017;10:3-7.
Korver DR, Klasing KC. Dietary fish oil alters spesific and
infalammatory immune responses in chicks. J Nutr 1997;127:2039-46.
Kresnoadi U. Toll-Like Receptor 2 as Signaling Pathway for Alveolar
Bone Osteogenesis Induced with Combination of Aloe Vera and Graft.
Dissertation. Surabaya: Airlangga University; 2012.
Pathak D. Vitamin C Protects, Maintains Healthy Bone Mass. Huston:
Baylor College of Medicine; 2010.
Rastogi S, Iqbal MS, Ohri D. In vitro study of anti-inflammatory and
antioxidant activity of some medicinal plants and their interrelationship.
Asian J Pharma Clin Res 2018;11:195-202.
Joshua JM, Anikumar A, Cu V, Vasudevan DT, Surendran SA.
Evaluation of the effect of different concentrations of Aloe vera on
inflammation and re-epithelialization in diabetic ulcers in a rat model.
Asian J Pharm Clin Res 2018;11:25-32.
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