EFFECT OF NANOCURCUMIN ON CISPLATIN-INDUCED ACUTE KIDNEY INJURY: A FOCUS ON NRF2 AND KEAP1
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.32Keywords:
Cisplatin, Keap1, Nanocurcumin, Nephrotoxicity, Nrf2Abstract
Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1.
Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after
treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1.
Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was
upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop,
which upregulated transcription of Keap1.
Conclusion: NC increased Keap1 levels, but not significantly.
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