NOVEL TRANSETHOSOME CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAF EXTRACT FOR ENHANCED SKIN DELIVERY OF EPIGALLOCATECHIN GALLATE: FORMULATION AND IN VITRO PENETRATION TEST
Keywords:Cream, Epigallocatechin gallate, Green tea leaf extract, Penetration, Transethosome
Objective: This study aimed to formulate a transethosome cream (TEC) to increase skin penetration of epigallocatechin gallate (EGCG) in green tea
leaf extract and evaluate their physicochemical characteristics and skin penetration capacity.
Methods: Transethosomes were prepared through thin-layer hydration method in three formulations (F1âˆ’F3) with different Span 80 and ethanol concentrations.
Transethosome morphology was characterized using transmission electron microscopy, particle size, polydispersity (PDI), and zeta potential using a particle
size analyzer and entrapment efficiency (EE). Penetration was tested using an in vitro Franz diffusion cell with female Sprague-Dawley rat skin as the
Results: Transethosome F2 containing green tea extract equivalent to 3% EGCG, 4% Lipoid P30, 0.75% Span 80, and 30% ethanol had the best
characteristic including spherical shape, smallest particle size (35.35 nm), 0.319 PDI, zeta potential of âˆ’29.97Â±3.05 mV, and highest EE of
45.26%Â±8.15%. TEC permitted greater flux than non-TEC (first phase: 60.56Â±4.52 vs. 25.69Â±0.83 Î¼gâ€¢cmâˆ’2â€¢hâˆ’1 and second phase: 23.13Â±1.38 vs.
Conclusion: Transethosome can increase the skin penetration of green tea leaf extract.
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