• YULITA BONG Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • SRI ANGKY SOEKANTO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ERIK IDRUS Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia




Bone, calcium, Centella asiatica, Phytoestrogen, Osteoporosis


Objective: This study aimed to evaluate the efficacy of Centella asiatica leaf extract in maintaining calcium and phosphate levels in the bone for
preventing osteoporosis in postmenopausal women.
Methods: The obtained C. asiatica leaf extract was administered to ovariectomized rats at the doses of 60, 120, and 180 mg/kg body weight (BW)
for 30 days. Subsequently, the rats were euthanized by ether overdose, and bone calcium and phosphate levels were determined by the wet ashing
technique and spectrophotometry.
Results: The results showed no significant differences in the bone calcium and phosphate levels among rats administered with different doses of
C. asiatica leaf extract.
Conclusion: Our results indicate that C. asiatica leaf extract cannot be used as a source of phytoestrogens to maintain calcium and phosphate levels
in the bones.


Download data is not yet available.


1. Mauck K, Clarke B. Concise review for clinicians diagnosis, screening,
prevention, and treatment of osteoporosis. Mayo Clin Pro 2006;81:662-72.
2. Ismal NM. Postmenopausal osteoporosis: Epidemiology,
pathophysiology and treatment. Malays J Pathol 1997;19:21-5.
3. Simon L. Osteoporosis. Rheum Dis Clin N Am 2007;33:149-76.
4. Sipos W, Peter P, Martina R. Pathophysiology of osteoporosis.
Themenschwerpunkt 2009;159:230-4.
5. Nakamura T, Imai Y, Matsumoto T, Sato S. Estrogen prevents bone loss
via estrogen receptor ? and induction of Fas ligand in osteoclasts. Cell
6. Zheng C, Qin L. Chemical components of Centella asiatica and their
bioactivities. J Chin Integr Med 2007;5:348-51.
7. US Department of Health and Human Services. Facts about Menopausal
Hormone Therapy. Available from: http://www.nhlbi.nih.gov/health/
women/pht_facts.pdf. [Last accessed on 2013 Sep 20].
8. Dixon RA. Phytoestrogens. Annu Rev Plant Biol 2004;55:225-61.
9. Athena Urology and Urogynecology. What is Phytoestrogen. Available
from: http://www.athena-urology.com/patient-docs/Phytoestrogen-seattlefemale-
urologists-urogynecologists.pdf. [Last accessed on 2013 Sep 20].
10. Barnes J, Anderson L, Phillipson J. Herbal Medicines. Pharm Press
11. Winarto WP, Surbakti M. The Efficacy and Use of Centella asiatica.
Jakarta: Agro Media Pustaka; 2005.
12. Roy D, Barman S, Shalk M. Current updates on Centella asiatica L.:
Phytochemistry, pharmacology and traditional uses. Med Plant Res
13. Subban R, Veerakumar A, Manimaran R, Hashim KM, Balachandran I.
Two new flavonoids from Centella asiatica (Linn.). J Nat Med
14. Wong R, Rabie A. Effect of quercetin on preosteoblast and bone defects.
Open Orthop J 2008;2:27-32.
15. Guo AJ, Choi RC, Zheng KY, Chen VP, Dong TT, Wang ZT, et al.
Kaempferol as a flavonoid induces osteoblastic differentiation via
estrogen receptor signaling. Chin Med 2012;7:10.
16. Wattel A, Kamel S, Mentaverri R, Lorget F, Prouillet C, Petit JP, et al.
Potent inhibitory effect of naturally occuring flavonoids quercetin and
kaempferol on in vitro osteoclastic bone resorption. Biochem Pharm
17. Bakhsh A, Mustapha NM, Mohamed S. Catechin-rich oil palm leaf
extract enhances bone calcium content of estrogen-deficient rats.
Nutrition 2013;29:667-72.
18. Kamon M, Zhao R, Sakamoto K. Green tea polyphenol (?)
epigallocatechin gallate suppressed the differentiation of murine
osteoblastic MC3T3-E1 cells. Cell Biol Int 2010;34:109-16.
19. Horcajada-Molteni M, Crespy V, Coxam V, Davicco MJ, Rémésy C,
Barlet JP. Rutin inhibits ovariectomy-induced osteopenia in rats. J Bone
Miner Res 2000;15:2251-8.
20. Hirata M, Matsumoto C, Morichika T, Miyaura C, Inada M. Naringin
suppresses osteoclast formation and enhance bone mass in mice.
J Health Sci 2009;55:463-7.
21. Wang J, Liao Y, Fan J, Ye T, Sun X, Dong S. Apigenin inhibits the
expression of IL-6, IL-8, and ICAM-1 in DEHP-stimulated human
umbilical vein endothelial cells in vivo. Inflammation 2012;35:1466-76.
22. Bhavna D, Jyoti K. Centella asiatica L.: The elixir of life. Int J Res
Ayurveda Pharm 2011;2:431-8.
23. Umland EM, Cauffield JS, Kirk JK, Thomason TE. Phytoestrogens
as therapeutic alternatives to traditional hormone replacement in
postmenopausal women. Pharmacotherapy 2000;20:981-90.
24. Helmberg A. Bone Metabolism 2009-2010. p. 13. Available from:
http://www.helmberg.at/bone-metabolism.htm. [Last accessed on
2000 Aug 20].
25. University of Cambridge. Structure and Composition of Bone 2004-
2013. Available from: http://www.doitpoms.ac.uk/tlplib/bones/
structure.php[Last accessed on 2013 Nov 01].
26. Borer K. Physical activity in prevention and amelioration of
osteoporosis in women. Sports Med 2005;35:779-830.
27. Matkovic V, Fontana D, Tominac C, Goel P, Chesnut CH 3rd. Factors
that influence peak bone mass formation: A study of calcium balance
and the inheritance of bone mass in adolescent females. Am J Clin Nutr
28. Rakhman L, Pangestika O, Fitriani D, Rudhanton R, Permatasari N.
Aloe vera ethanol extract as a therapy for alveolar mandible regeneration
in lipopolysaccharide-exposed rats. Int J App Pharm 2018;10:11-4.
29. Sivamaruthi B, Kesika P, Chaiyasut C. A review on anti-aging properties
of probiotics. Int J App Pharm 2018;10:23-7.



How to Cite

BONG, Y., SOEKANTO, S. A., & IDRUS, E. (2019). EFFECTS OF CENTELLA ASIATICA (L.) LEAF EXTRACT ON BONE CALCIUM AND PHOSPHATE LEVELS OF OVARIECTOMIZED RATS. International Journal of Applied Pharmaceutics, 11(1), 67–70. https://doi.org/10.22159/ijap.2019.v11s1.AR169



Original Article(s)

Most read articles by the same author(s)

1 2 > >>