THE EFFECTS OF PROPOLIS HONEY CANDY CONSUMPTION ON MYELOPEROXIDASE ACTIVITY IN STIMULATED SALIVA

DARIN SAFINAZ; SRI ANGKY SOEKANTO; AGOENG TJAHJANI SARWONO

doi:10.22159/ijap.2019.v11s1.185

Authors

DARIN SAFINAZ Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
SRI ANGKY SOEKANTO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
AGOENG TJAHJANI SARWONO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.185

Keywords:

Flavonoid, Myeloperoxidase activity, Propolis

Abstract

Objective: Propolis is a natural product that contains flavonoids and has antibacterial effects that could decrease myeloperoxidase (MPO) activity in
the saliva. Propolis honey candy is currently being developed and to analyze the effects of propolis honey candy on MPO activity in stimulated saliva.
Methods: Stimulated saliva samples were collected from individuals who met the inclusion criteria before and after consumption of propolis honey
candy twice a day for 7 days. Salivary samples were centrifuged to separate the supernatant and pellet. A 100-μl aliquot of the supernatant was
directly added to the wells of a 96-well plate and mixed with 100 μl of substrate solution containing 3,3’-diaminobenzidine, guaiacol, dapsone, and
Tris-HCl buffer. After incubation for 30 min at room temperature, MPO activity was measured by subtracting the absorbance value (wavelength of
450 nm) of the saliva samples from that of the blank control (distilled water).
Results: The absorbance value of MPO activity of propolis honey candy was 0.071 before consumption and 0.076 after consumption.
Conclusion: MPO activity significantly increased after the consumption of propolis honey candy (Wilcoxon signed-rank test, p<0.05).

Downloads

Download data is not yet available.

References

1. Kumala SL. Utilization of traditional medicines with benefits and safety
considerations [In Indonesia]. Maj Ilmu Kefarmasian 2006;3:1-7.
2. Ramdhon F. Mahkota Dewa Red Ginger, Sambiloto, Secang, Natural
Medicine: Chemical Medicines vs Herbal Medicines; 2007. Available
from: http://www.jahemerah.com/2007/05/obat-kimia-vs-obat-herbal.
html. [Last accessed on 2014 Jun 07].
3. Bogdanov S. Propolis: Composition, Health, Medicine: A Review. Bee
Product Science; 2012. Available from: http://www.bee-hexagon.net.
4. Susanto A. In: Indriani HKS, editors. Honey Therapy. Jakarta: Niaga
Swadaya; 2007. p. 83-4.
5. Shiba Y, Kinoshita T, Chuman H, Taketani Y, Takeda E, Kato Y,
et al. Flavonoids as substrates and inhibitors of myeloperoxidase:
Molecular actions of aglycone and metabolites. Chem Res Toxicol
2008;21:1600-9.
6. Tsai YC, Wang YH, Liou CC, Lin YC, Huang H, Liu YC, et al.
Induction of oxidative DNA damage by flavonoids of propolis: Its
mechanism and implication about antioxidant capacity. Chem Res
Toxicol 2012;25:191-6.
7. Edgar WM. Saliva: Its secretion, composition and functions. Br Dent J
1992;172:305-12.
8. de Almeida Pdel V, Grégio AM, Machado MA, de Lima AA,
Azevedo LR. Saliva composition and functions: A comprehensive
review. J Contemp Dent Pract 2008;9:72-80.
9. Amerogen AV, Abyono R. Salivary Saliva and Glands Meaning for
Dental Health. Yogyakarta: Gajah Mada University; 1991. p. 1-56.
10. Whittenbury R. Hydrogen peroxide formation and catalase activity in
the lactic acid bacteria. J Gen Microbiol 1964;35:13-26.
11. Pruit KM, Reiter B. Biochemistry of peroxidase system antimicrobial
effects. In: Pruit KM, Tenovuo JO, editors. The Lactoperoxidase
System: Chemistry and Biological Significance (Immunology). 1st ed.
New York: CRC Press; 1985. p. 272.
12. Sahlan M, Ramadhan M. Hard Candy Propolis for Oral Health. Depok:
Universitas Indonesia; 2011. p. 1-2, 5.
13. Gladea Z. The Effect of Consumption of Heoney Propolis Candy on
the Prevalence of Streptococcus mutans in Patients with Dental Caries.
Jakarta: Universitas Indonesia; 2012. p. 26-7.
14. Boufadi YM, Soubhye J, Riazi A, Rousseau A, Vanhaeverbeek M,
Nève J, et al. Characterization and antioxidant properties of six Algerian
propolis extracts: Ethyl acetate extracts inhibit myeloperoxidase
activity. Int J Mol Sci 2014;15:2327-45.
15. Sakamoto W, Fujii Y, Kanehira T, Asano K, Izumi H. A novel assay
system for myeloperoxidase activity in whole saliva. Clin Biochem
2008;41:584-90.
16. Axelsson L. Lactic acid bacteria: Classification and physiology. In:
Salminen S, Wright AV, Ouwehand A, editors. Lactic Acid Bacteria.
3rd ed. New York: Marcel Dekker Press; 2004.
17. Raba J, Mottola HA. Glucose oxidase as an analytical reagent. Crit Rev
Anal Chem 2006;25:1-42.
18. Doner LW. The sugars of honey-a review. J Sci Food Agric
1977;28:443-56.
19. Soekanto SA, Rosithahakiki N, Suniarti DF, Sahlan M. Comparison of
the potency of several fluoride-based varnishes as an anticariogenic
on calcium, phosphate, and fluoride ion levels. Int J Appl Pharm
2017;9:55-9.
20. Soekanto SA, Fadillah F, Nuraisiya P, Gultom F, Sarwono AT. The
potential of several fluoride-based varnishes as remineralization agents:
Morphological studies, dentin surface hardness, and crystallinity tests.
Int J Appl Pharm 2017;9:60-6.