IN VITRO ANTIMALARIAL POTENCY OF ELEUTHERINE BULBOSA AND ITS EFFECT ON THE MORPHOLOGY OF PLASMODIUM FALCIPARUM

LUH SESOTYANING N.; MELVA LOUISA; HENDRI ASTUTY; DIN SYAFRUDDIN; PUJI BUDI SETIA ASIH

doi:10.22159/ijap.2019.v11s1.14

Authors

LUH SESOTYANING N. Department of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
MELVA LOUISA Department of Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
HENDRI ASTUTY Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
DIN SYAFRUDDIN Malaria and Vector Resistance Unit, Eijkman Institute for Molecular Biology, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.
PUJI BUDI SETIA ASIH Malaria and Vector Resistance Unit, Eijkman Institute for Molecular Biology, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.14

Keywords:

Eleutherine bulbosa, Antimalarial, Toxicity, 50 Inhibition concentration

Abstract

Objective: This study aimed to determine the potential inhibitory effects of Eleutherine bulbosa on the growth of Plasmodium falciparum and its toxic
effects on lymphocyte cells.
Methods: We performed a 50% inhibition concentration (IC50) test of E. bulbosa against P. falciparum, used transmission electron microscopy (TEM)
to observe Plasmodium morphology after exposure to E. bulbosa, and determined E. bulbosa’s cytotoxic concentration 50% (CC50) against lymphocytes.
Results: The IC50 value of E. bulbosa was 0.62 μg/ml. We observed crystals in the mitochondria of parasites under TEM. CC50 values at 24 h and 72 h
were 851.14 μg/ml and 707.94 μg/ml, respectively.
Conclusion: The main content of E. bulbosa is naphthoquinones that are suspected of having a mitochondrial target on the malaria parasite. E. bulbosa
is not toxic to lymphocytes; therefore, it has a potential as an antimalarial therapeutic.

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