THE EFFECT OF CARBOXYMETHYL CHITOSAN/AMORPHOUS CALCIUM PHOSPHATE TO GUIDE TISSUE REMINERALIZATION OF DENTIN COLLAGEN

ROSDIANA NURUL ANNISA; NILAKESUMA DJAUHARIE; ENDANG SUPRASTIWI; NORMA AVANTI

doi:10.22159/ijap.2019.v11s1.16300

Authors

ROSDIANA NURUL ANNISA Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
NILAKESUMA DJAUHARIE Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
ENDANG SUPRASTIWI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
NORMA AVANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.16300

Keywords:

Carboxymethyl chitosanamorphous calcium phosphate, Guided tissue remineralization, Intrafibrillar remineralization

Abstract

Objective: Carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) can replace the role of dentine matrix protein 1. Guided tissue
remineralization (GTR) is a method of extrafibrillar and intrafibrillar collagen remineralization. This study analyzed the ability of CMC/ACP to achieve
intrafibrillar and extrafibrillar remineralization on demineralized dentin.
Methods: We divided 12 demineralized occlusal cavities into four groups: 7 days control group, 14 days control group, 7 days CMC/ACP group, and
14 days CMC/ACP group. In the control group, the cavities were directly filled with temporary restoration material, while the cavities of the CMC/ACP
groups first underwent application of CMC/ACP and were then filled with temporary restoration material. All samples were stored in a 37°C shaking
incubator. 12 samples were analyzed by energy-dispersive X-ray (EDX) spectroscopy and four were analyzed by transmission electron microscope
(TEM).
Results: From day 7 to day 14, there was a significant increase in calcium and phosphate levels within the CMC/APC groups. The phosphate levels
were much lower than the calcium or minerals formed on the 7th day, in the form of hydroxyapatite. TEM analysis shows that the CMC/ACP group
exhibited more intrafibrillar and extrafibrillar remineralization.
Conclusion: CMC/ACP can improve the GTR process.

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