POLYMORPHISM OF OSTEOPROTEGERIN GENE IN INDONESIAN MEN WITH PERIODONTITIS
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.18333Keywords:
Periodontitis, Osteoprotegerin, PolymorphismAbstract
Objective: This work aimed to clarify the association of the severity of periodontitis and polymorphism in osteoprotegerin (OPG) (T950C) in
Indonesian men.
Methods: For DNA extraction, blood serum samples were used from 100 consenting Indonesian males for whom also the status of periodontitis had
been classified as mild, moderate, or severe. Polymerase chain reaction and restriction fragment length polymorphism techniques were applied to
evaluate OPG (T950C) polymorphism, using Hind II restriction enzyme and electrophoresis on agarose gel to separate the indicative fragments.
Results: The genotype distribution of the OPG (T950C) polymorphism had an appearance of an increasing percentage of TT genotype (allele T) with
increasing severitPeriodontitisy of periodontitis. The CC genotype was relatively rare (1%) in the tested Indonesian male population.
Conclusions: The results show no significant association between the severity of periodontitis and polymorphism of OPG (T950C) in Indonesian men.
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References
1. Statistics Survey. Survey of Demographic and Health in Indonesia.
Available from: http://www.datastatistik-indonesia.com/sdki. [Last
accessed on 2011 Oct 09].
2. Loe H, Morrison E. Epidemiology of Periodontal Disease. Contemp
Periodontics 2003;2003:106-16.
3. Rooban T, Rao A, Joshua E, Ranganathan K. Dental and oral health
status in drug users in Chennai, India: A cross sectional study. J Oral
MaxilloFac Pathol 2008;12:16-21.
4. Newman MG. The Normal Periodontium. Carranza’s Clinical
Periodontology. St. Louis, Missouri: Elsevier Saunders; 2003. p. 16.
5. Offenbacher S, Barros SP, Beck JD. Rethinking periodontal
inflammation. J Periodontol 2008;79:1577-84.
6. Borrell LN, Papapanou PN. Analytical epidemiology of periodontitis.
J Clin Periodontol 2005;32 Suppl 6:132-58.
7. Michalowicz BS, Diehl SR, Gunsolley JC, Sparks BS, Brooks CN,
Koertge TE, et al. Evidence of a substantial genetic basis for risk of
adult periodontitis. J Periodontol 2000;71:1699-707.
8. Michalowicz BS, Aeppli D, Virag JG, Klump DG, Hinrichs JE,
Segal NL, et al. Periodontal findings in adult twins. J Periodontol
1991;62:293-9.
9. Tabor HK, Risch NJ, Myers RM. Candidate-gene approaches for
studying complex genetic traits: Practical considerations. Nat Rev
Genet 2002;3:391-7.
10. Mogi M, Otogoto J, Ota N, Togari A. Differential expression of
RANKL and osteoprotegerin in gingival crevicular fluid of patients
with periodontitis. J Dent Res 2004;83:166-9.
11. Crotti T, Smith MD, Hirsch R, Soukoulis S, Weedon H, Capone M, et al.
Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG)
protein expression in periodontitis. J Periodontal Res 2003;38:380-7.
12. Bostanci N, Ilgenli T, Emingil G, Afacan B, Han B, Töz H, et al.
Gingival crevicular fluid levels of RANKL and OPG in periodontal
diseases: Implications of their relative ratio. J Clin Periodontol
2007;34:370-6.
13. Auerkari EI, Suryandari DA, Umami SS, Kusdhany LS, Siregar TW,
Rahardjo TB, et al. Gene promoter polymorphism of RUNX2 and risk
of osteoporosis in postmenopausal indonesian women. SAGE Open
Med 2014;2:2050312114531571.
14. Auerkari E, Suhartono A, Djamal N, Verisqa F, Suryandari D,
Kusdhany L, et al. CRP and IL-1B gene polymorphisms and CRP in
blood in periodontal disease. Open Dent J 2013;7:88-93.
15. Tanjaya J, Auerkari EI. IL-1? genetic polimorphism in menopause
women as periodontal disease risk factor. J Dent Indones 2011;18:1-5.
16. Auerkari EI, Kusdhany L, Umami SS, Rahardjo TB, Talbot C.
Polymorphism of methylenetetrahydrofolate reductase (a1298c) as
a risk factor for osteoporosis in post-menopausal indonesian women.
Asian J Pharm Clin Res 2017;10:172-5.
17. Park OJ, Shin SY, Choi Y, Kim MH, Chung CP, Ku Y, et al. The
association of osteoprotegerin gene polymorphisms with periodontitis.
Oral Dis 2008;14:440-4.
18. Baioni CS, de Souza CM, Ribeiro Braosi AP, Luczyszyn SM,
Dias da Silva MA, Ignácio SA, et al. Analysis of the association of
polymorphism in the osteoprotegerin gene with susceptibility to chronic
kidney disease and periodontitis. J Periodontal Res 2008;43:578-84.
19. Wagner J, Kaminski WE, Aslanidis C, Moder D, Hiller KA,
Christgau M, et al. Prevalence of OPG and IL-1 gene polymorphisms
in chronic periodontitis. J Clin Periodontol 2007;34:823-7.
20. Park OJ, Shin SY, Choi Y, Kim MH, Chung CP, Ku Y, et al. The
association of osteoprotegerin gene polymorphisms with periodontitis.
Oral Dis 2008;14:440-4.
21. Wohlfahrt JC, Wu T, Hodges JS, Hinrichs JE, Michalowicz BS. No
association between selected candidate gene polymorphisms and severe
chronic periodontitis. J Periodontol 2006;77:426-36.
22. Soedarsono N, Rabello D, Kamei H, Fuma D, Ishihara Y, Suzuki M,
et al. Evaluation of RANK/RANKL/OPG gene polymorphisms in
aggressive periodontitis. J Periodontal Res 2006;41:397-404.
Available from: http://www.datastatistik-indonesia.com/sdki. [Last
accessed on 2011 Oct 09].
2. Loe H, Morrison E. Epidemiology of Periodontal Disease. Contemp
Periodontics 2003;2003:106-16.
3. Rooban T, Rao A, Joshua E, Ranganathan K. Dental and oral health
status in drug users in Chennai, India: A cross sectional study. J Oral
MaxilloFac Pathol 2008;12:16-21.
4. Newman MG. The Normal Periodontium. Carranza’s Clinical
Periodontology. St. Louis, Missouri: Elsevier Saunders; 2003. p. 16.
5. Offenbacher S, Barros SP, Beck JD. Rethinking periodontal
inflammation. J Periodontol 2008;79:1577-84.
6. Borrell LN, Papapanou PN. Analytical epidemiology of periodontitis.
J Clin Periodontol 2005;32 Suppl 6:132-58.
7. Michalowicz BS, Diehl SR, Gunsolley JC, Sparks BS, Brooks CN,
Koertge TE, et al. Evidence of a substantial genetic basis for risk of
adult periodontitis. J Periodontol 2000;71:1699-707.
8. Michalowicz BS, Aeppli D, Virag JG, Klump DG, Hinrichs JE,
Segal NL, et al. Periodontal findings in adult twins. J Periodontol
1991;62:293-9.
9. Tabor HK, Risch NJ, Myers RM. Candidate-gene approaches for
studying complex genetic traits: Practical considerations. Nat Rev
Genet 2002;3:391-7.
10. Mogi M, Otogoto J, Ota N, Togari A. Differential expression of
RANKL and osteoprotegerin in gingival crevicular fluid of patients
with periodontitis. J Dent Res 2004;83:166-9.
11. Crotti T, Smith MD, Hirsch R, Soukoulis S, Weedon H, Capone M, et al.
Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG)
protein expression in periodontitis. J Periodontal Res 2003;38:380-7.
12. Bostanci N, Ilgenli T, Emingil G, Afacan B, Han B, Töz H, et al.
Gingival crevicular fluid levels of RANKL and OPG in periodontal
diseases: Implications of their relative ratio. J Clin Periodontol
2007;34:370-6.
13. Auerkari EI, Suryandari DA, Umami SS, Kusdhany LS, Siregar TW,
Rahardjo TB, et al. Gene promoter polymorphism of RUNX2 and risk
of osteoporosis in postmenopausal indonesian women. SAGE Open
Med 2014;2:2050312114531571.
14. Auerkari E, Suhartono A, Djamal N, Verisqa F, Suryandari D,
Kusdhany L, et al. CRP and IL-1B gene polymorphisms and CRP in
blood in periodontal disease. Open Dent J 2013;7:88-93.
15. Tanjaya J, Auerkari EI. IL-1? genetic polimorphism in menopause
women as periodontal disease risk factor. J Dent Indones 2011;18:1-5.
16. Auerkari EI, Kusdhany L, Umami SS, Rahardjo TB, Talbot C.
Polymorphism of methylenetetrahydrofolate reductase (a1298c) as
a risk factor for osteoporosis in post-menopausal indonesian women.
Asian J Pharm Clin Res 2017;10:172-5.
17. Park OJ, Shin SY, Choi Y, Kim MH, Chung CP, Ku Y, et al. The
association of osteoprotegerin gene polymorphisms with periodontitis.
Oral Dis 2008;14:440-4.
18. Baioni CS, de Souza CM, Ribeiro Braosi AP, Luczyszyn SM,
Dias da Silva MA, Ignácio SA, et al. Analysis of the association of
polymorphism in the osteoprotegerin gene with susceptibility to chronic
kidney disease and periodontitis. J Periodontal Res 2008;43:578-84.
19. Wagner J, Kaminski WE, Aslanidis C, Moder D, Hiller KA,
Christgau M, et al. Prevalence of OPG and IL-1 gene polymorphisms
in chronic periodontitis. J Clin Periodontol 2007;34:823-7.
20. Park OJ, Shin SY, Choi Y, Kim MH, Chung CP, Ku Y, et al. The
association of osteoprotegerin gene polymorphisms with periodontitis.
Oral Dis 2008;14:440-4.
21. Wohlfahrt JC, Wu T, Hodges JS, Hinrichs JE, Michalowicz BS. No
association between selected candidate gene polymorphisms and severe
chronic periodontitis. J Periodontol 2006;77:426-36.
22. Soedarsono N, Rabello D, Kamei H, Fuma D, Ishihara Y, Suzuki M,
et al. Evaluation of RANK/RANKL/OPG gene polymorphisms in
aggressive periodontitis. J Periodontal Res 2006;41:397-404.
