EFFECT OF HIBISCUS SABDARIFFA LINN ON IL-6 AND TNF- α LEVELS IN OVERTRAINED RAT HEART

DEWI IRAWATI SOERIA SANTOSO; SRI YUNITA; NURUL PARAMITA; TRINOVITA ANDRAINI; NENG TINE KARTINAH; GUSHAN FAHMI EL BAYANI; ERMITA I. IBRAHIM ILYAS

doi:10.22159/ijap.2019.v11s6.33535

Authors

DEWI IRAWATI SOERIA SANTOSO Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
SRI YUNITA Master Program in Biomedical Sciences, Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
NURUL PARAMITA Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
TRINOVITA ANDRAINI Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
NENG TINE KARTINAH Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
GUSHAN FAHMI EL BAYANI Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia,
ERMITA I. IBRAHIM ILYAS Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019.v11s6.33535

Keywords:

Overtraining, Interleukin-6, Tumor Necrosis Factor-alpha, Hibiscus sabdariffa Linn

Abstract

Objective: This study aims to determine the effect of Hibiscus sabdariffa Linn. (HSL) administration on the interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) levels in rat heart. Overtraining was proven to increase the IL-6 and TNF-α levels in the blood, and HSL had anti-inflammatory and anti-oxidant properties. However, no studies have been conducted on the effect of methanolic extract of HSL administration on the IL-6 and TNF-α levels in overtrained rat heart.

Methods: This study used 25 male adult Wistar rats aged 8–10 w and weighing 200–250 g. The rats were randomly divided into five groups: control (C), control H. sabdariffa Linn (C+HSL), overtraining (OT), overtraining H. sabdariffa Linn (OT+HSL), and aerobic (A). Treatment was given 5 times a week for 11 w. At the end of the study, the IL-6 and TNF-α levels were measured using a standard ELISA kit.

Results: IL-6 and TNF-α levels in the heart were the highest in the overtraining group. The group that received HSL administration showed the lowest TNF-α and IL-6 levels.

Conclusion: HSL could be a used to protect the heart from an inflammatory state, particularly in an overtraining condition.

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