RAPID AUTHENTICATION OF TURMERIC POWDER ADULTERATED WITH CURCUMA ZEDOARIA AND CURCUMA XANTHORRHIZA USING FTIR-ATR SPECTROSCOPY AND CHEMOMETRICS

ELLSYA ANGELINE; RATNA ASMAH SUSIDARTI; ABDUL ROHMAN

doi:10.22159/ijap.2019v11i5.33701

Authors

ELLSYA ANGELINE Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia
RATNA ASMAH SUSIDARTI Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia
ABDUL ROHMAN Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia, Institut of Halal Industry and Systems, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

DOI:

https://doi.org/10.22159/ijap.2019v11i5.33701

Keywords:

Curcuma longa, Curcuma zedoaria, Curcuma xanthorrhiza, FTIR spectroscopy, Chemometrics

Abstract

Objective: The objective of this study is to develop a rapid, simple, non-destructive and inexpensive analytical method using Fourier Transform Infrared (FTIR) spectroscopy with Attenuated Total Reflection (ATR) as a sampling technique, combined with chemometrics for authentication of turmeric powder adulterated with Curcuma zedoaria and Curcuma xanthorrhiza.

Methods: Turmeric powder is placed above the diamond crystal in ATR compartment. Spectra are scanned in the absorbance mode from 4000 to 600 cm^-1. The obtained spectra is further analyzed by Principal Component Analysis (PCA), Partial Least Square Discriminant Analysis (PLS-DA), and Partial Least Square Regression (PLS-R).

Results: PCA score plot shows that Curcuma longa, Curcuma zedoaria, and Curcuma xanthorrhiza can be discriminated well. PLS-DA can be used to build the model for classification between pure turmeric powder and adulterated powder with the values of Q², R²X, and R²Y of 0.9558, 0.9813, and 0.9746, respectively. The good calibration model for quantification of each adulterant is obtained by PLS-R with R² value more than 0.99 and lower RMSEC value. Both models have been validated by internal and external validation which result in the high R² value and low RMSEP value which indicates that both models are accurate and precise.

Conclusion: The combination of FTIR-ATR spectroscopy and chemometrics can be used to authenticate turmeric powder adulterated with Curcuma zedoaria and Curcuma xanthorrhiza.

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