BIO-NANOPARTICLES: GREEN SYNTHESIS OF GOLD NANOPARTICLES AND ASSESSMENT OF BIOLOGICAL EVALUATION

RATIH DYAH PERTIWI; SUWALDI; ERNA PRAWITA SETYOWATI; RONNY MARTIEN

doi:10.22159/ijap.2019v11i6.34826

Authors

RATIH DYAH PERTIWI Department of Pharmacy, Faculty of Health Sciences, Universitas Esa Unggul, Jl Arjuna no 9A Jakarta, 11510, Doctoral Program in Pharmaceutical Science, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
SUWALDI Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, 55281
ERNA PRAWITA SETYOWATI Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
RONNY MARTIEN Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, 55281

DOI:

https://doi.org/10.22159/ijap.2019v11i6.34826

Keywords:

Green synthesis, Gold Nanoparticles, Antioxidant, Muntingia calabura, Biological activities

Abstract

Objective: The design like bio-nano particles are beneficial over chemical and physical composition due to the eco-friendly and lower-cost synthesis of nanoparticles. The current study was purposed for the biosynthesis of gold nanoparticles (GNPs) and their antioxidant evaluation.

Methods: Aqua extract of Muntingia calabura, Linn was applied for the synthesis of GNPs and confirmed by UV visible and ATR-Fourier Transform Infra-Red (ATR-FTIR) spectroscopy. Transmission Electron Microscope and Particle Size Analyser were used for the shape zeta potential and determination of size. Antioxidant activity was examined by DPPH (1,1 diphenyl-2-picryl-hydrazyl) radical scavenging method.

Results: The result showed that UV–vis absorption spectra of H Au Cl₄ at 290 nm while absorption spectra of biosynthesis gold nanoparticles at 540 nm. The forming of nanoparticles were spherical, having an average particle size of 88 nm, and the result of zeta potential was 9.5 mV. Analysis of ATR-FTIR revealed the possible involvement of phytochemical constituents in gold nanoparticles of aqua extract. Green synthesized nanoparticles showed enhanced antioxidant properties.

Conclusion: Green synthesized GNPs showed enhanced biological activities. Present results also support the benefit of using the biosynthesis method for the production of gold nanoparticles that have the potential of antioxidant and biology activities.

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References

P Balashanmugam, K Pudupalayam Thangavelu. Biosynthesis characterization of silver nanoparticles using cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity. Int J Nanomed 2015. p. 87.

MS Madhumithra, B Mosachristas, Tamil Selvi, Subashini. In vitro cytotoxicity of biosynthesized gold nanoparticles from shells of pistacia vera L. Int J Appl Pharm 2018;10:162.

S Ikram. Synthesis of gold nanoparticles using plant extract: an overview. Arch Med 2015;1:5.

S Ahmed, M Ahmad, BL Swami, S Ikram. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 2016;7:17-28.

S Duadarah, K Pujari, RD Durai, VHB Narayanan. Nanotechnology-based cosmeceuticals: a review. Int J Appl Pharm 2015;8:8–12.

Y Zeiri, P Elia, R Zach, S Hazan, S Kolusheva, Z Porat. Green synthesis of gold nanoparticles using plant extracts as reducing agents. Int J Nanomed 2014. p. 4007.

P Raveendran, J Fu, SL Wallen. Completely ‘green’ synthesis and stabilization of metal nanoparticles. J Am Chem Soc 2003;125:13940–1.

J Kasthuri, S Veerapandian, N Rajendiran. Biological synthesis of silver and gold nanoparticles using apiin as reducing agent. Colloids Surf B Biointerfaces 2009;68:55–60.

D Philip. Green synthesis of gold and silver nanoparticles using Hibiscus rosa sinensis. Phys E Low Dimens Syst Nanostructures 2010;42:1417–24.

M Sechi. Single-step green synthesis and characterization of gold-conjugated polyphenol nanoparticles withandnbsp; antioxidantandnbsp; and biological activities. Int J Nanomed 2014;23:4935-51.

K Marimuthu, R Dhanalakshmi. Qualitative and quantitative study of phytochemicals in Muntingia calabura L. leaf and fruit. World J Pharm Res 2014;3:1687–96.

D Ramada. Antioxidant activity: root, leaves and fruits aqueous extracts of Muntingia calabura; 2015.

BH Nugroho, S Suparmi, MR Syifaudin. Preparation and characterization of gold nanoparticles lamtoro extract (Leucaena leucocephala (Lam.) de Wit) with eco-friendly biosynthesis process,” presented at the 2nd international conference on chemistry, chemical process and engineering (IC3PE), Yogyakarta, Indonesia; 2018. p. 020076.

JS Djajadisastra, P Purnamasari, A Pujiyanto. Antioxidant activity of gold nanoparticles using gum arabic as a stabilizing agent. Int J Pharm Pharm Sci 2014;6:462–5.

DOB Apriandanu, Y Yulizar. The role of aqueous leaf extract of Tinospora crispa as reducing and capping agents for synthesis of gold nanoparticles. IOP Conf Ser Mater Sci Eng 2017;188:012013.

K Preethi, N Vijayalakshmi, R Shamna, JM Sasikumar. In vitro antioxidant activity of extracts from fruits of Muntingia calabura Linn. from India. Pharmacogn J 2010;2:11–8.

S Ahmed, Annu S Ikram, S Yudha S. Biosynthesis of gold nanoparticles: a green approach. J Photochem Photobiol B 2016;161:141–53.

P Kuppusamy, MM Yusoff, GP Maniam, N Govindan. Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications–an updated report. Saudi Pharm J 2016;24:473–84.

AK Mittal, Y Chisti, UC Banerjee. Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 2013;31:346–56.

I Demiral, NG Atilgan, S Şensoz. Production of biofuel from soft shell of pistachio (Pistacia Vera, L.). Chem Eng Commun 2008;196:104–15.

SE McNeil. Ed. Characterization of Nanoparticles Intended for Drug Delivery. Vol. 697. Totowa, NJ: Humana Press; 2011.

P Arenas Guerrero. Determination of the size distribution of non-spherical nanoparticles by electric birefringence-based methods. Sci Rep 2018;8. Doi:10.1038/s41598-018-27840-0

MR Avadi. Preparation and characterization of insulin nanoparticles using chitosan and arabic gum with ionic gelation method. Nanomed Nanotechnol Biol Med 2010;6:58–63.

VJ Mohanraj, Y Chen. Nanoparticles-a review. Trop J Pharm Res 2006;5:561–73.

GS Banker, J Siepmann, C Rhodes. Modern pharmaceutics. CRC Press; 2002.

TC Shekhar, G Anju. Antioxidant activity by DPPH radical scavenging method of ageratum conyzoides linn. leaves. Am J Ethnomed 2014;1:244–9.

Md N Alam, N J Bristi, Md Rafiquzzaman. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013;21:143–52.