IN VITRO ANTI-PLATELET AGGREGATION ACTIVITY OF HYDROXYPROPYL CELLULOSE–CYSTEAMINE BASED NANOPARTICLES CONTAINING CRUDE BROMELAIN

Authors

  • DENI RAHMAT Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
  • LILIEK NURHIDAYATI Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
  • MARCELLA MARCELLA Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
  • ROS SUMARNY Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
  • DIAN RATIH LAKSMITAWATI Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020v12i3.35053

Keywords:

Crude bromelain, Anti-platelet aggregation, HPC-cysteamine, Nanoparticles

Abstract

Objective: The aim of the present study was to formulate bromelain into nanoparticles in order to improve its stability and activity.

Methods: Crude bromelain was prepared by protein precipitation from the pineapple stem juice using ammonium sulphate at the concentration of 60% (w/v). Nanoparticles containing crude bromelain were generated using the ionic gelation method with hydroxypropyl cellulose–cysteamine (HPC-cysteamine) conjugate as a matrix. Crude bromelain was then added to the HPC-cysteamine solution for ionic interaction to construct the nanoparticles, which were then analyzed for their particle size and zeta potential. The resulting nanoparticles were mixed with adenosine diphosphate (ADP) to perform anti-platelet aggregation.

Results: The nanoparticle had 928.3 nm in particle size and-7.25 mV in zeta potential. Anti-platelet activity of crude bromelain and the nanoparticles were determined with modification of light transmission aggregometry (LTA), in which ADP was used to induce an aggregation while a spectrophotometer UV-Vis was used to measure the absorbance at the wavelength of 600 nm. The result showed that crude bromelain and the nanoparticles rendered percentage inhibition of 8.00±1.17% and 48.56±11.19%, respectively.

Conclusion: Based on the result of a one-way analysis of variance (ANOVA), it was concluded that there was a significant difference in percentage inhibition between the two samples. The nanoparticles demonstrated a better anti-platelet aggregation activity compared to crude bromelain.

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References

Anderson KM, Odell PM, Wilson PWF, Kannel WB. Cardiovascular disease risk profiles. Am Heart J 2006;121:2293-8.

Putri RRRF, Ulfa EU, Riyanti R. Antiplatelet activity of red cabbage ethanolic extract (Brassica oleracea var. capitata L.). Pustaka Kesehatan 2013;2:111-4.

Hankey GJ, Eikelboom JW. Aspirin resistance. Lancet 2006;367:606-17.

Marmitt DJ, Bitencourt S, Silva ADCE, Goettert MI, Rempel C. Medicinal plants of RENISUS with analgesic activity. Crit Rev 2016;3:1-4.

Thiagarajan P, Jankowski JA. Aspirin and NSAIDs; benefits and harms for the gut. Best Prac Res Clin 2012;26:199-206.

Septiatin A. Fruits as medicinal plants. Bandung: CV Yrama Widya; 2009.

Murniati E. Sweet, scaly pineapple on the tongue. Surabaya: Surabaya Intellectual Club; 2012.

Heinicke RM, Gortner WA. Stem bromelain-a new protease preparation from pineapple plants. Econ Bot 1987;1:225-34.

Mulyono M, Rosmeilia E, Moi GJP, Valentine BO, Suhartono MT. Quantity and quality of bromelain in some Indonesian pineapple fruits. Int J Appl Bio-Pharm Tech 2013;4:235-9.

Rahmat D, Sakloetsakun D, Perera G, Schnürch AB. Design and synthesis of a novel cationic thiolated polymer. Int J Pharm 2011;411:10-7.

Bradford M. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle dye binding. Anal Biochem 1976;72:248-54.

Hall R, Mazer CD. Antiplatelet drugs: a review of their pharmacology and management in the perioperative period. Anesh Analg 2010;112:292-318.

Sathyapriya E, Velpandian V, Anbu J, Anjana A. In vitro antiplatelet aggregation activity and thrombolytic activity of Cheenalinga chendhuram. Int J Life Sci 2012;4:51-5.

Ghoshal K, Bhattacharyya M. Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis. Sci World J 2004:1-16. https://doi.org/10.1155/2014/781857

Rahmat D, Rahman FA, Nurhidayati L, Laksmitawati DR. Synthesis and characterization of hydroxypropyl cellulose-cysteamine conjugate as a novel cationic thiomer with lipophilic properties. Int J Appl Pharm 2019;11:222-6.

Muller C, Rahmat D, Sarti F, Leithner K, Schnürch AB. Immobilization of 2-mercaptoethylamine on oxidized chitosan: a substantially mucoadhesive and permeation enhancing polymer. J Mater Chem 2012;22:3899-908.

Ketnawa S, Chaiwut P, Rawdkuen S. Extraction of bromelain from pineapple peels. Food Sci Technol Int 2011;17:395-402.

Wu WC, Ng HS, Sun IM, Lan JCW. Single-step purification of bromelain from Ananas comosus pulp using a polymer/salt aqueous biphasic system. J Taiwan Inst Chem E 2017;79:158-62.

Abreu DCA, Figueiredo KCDS. Bromelain separation and purification processes from pineapple extract. Braz J Chem Eng 2019;36:1029-39.

Nouroozi RV, Noroozi MV, Ahmadizadeh M. Determination of protein concentration using Bradford microplate protein quantification assay. Int Electron J Med 2015;4:11-7.

Zor T, Selinger Z. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal Biochem 1996;236:302-8.

Gautam SS, Mishra SK, Dash V, Goyal AK, Rath G. Comparative study of extraction, purification and estimation of bromelain from stem and fruit of the pineapple. Plant Thai J Pharm Sci 2010;34:67-76.

Vellini M, Desideri D, Milanese A, Omini C, Daffonchio L, Hernandez A, et al. Possible involvement of eicosanoids in the pharmacological action of bromelain. Arzneimittel Forschung 1986;36:110-2.

Menon A, Priya VV, Gayathri R. Preliminary phytochemical analysis and cytotoxicity potential of pineapple extract on oral cancer cell lines. Asian J Pharm Clin Res 2016;9:140-3.

Hanif M, Zaman M, Qureshi S. Tiomers: a blessing to evaluating era of pharmaceuticals. Int J Polym Sci 2015;2015:1-9.

Dai Q, Zhu X, Yu J, Karangwa E, Xia S, Zhang X, et al. Mechanism of formation and stabilization of nanoparticles produced by heating electrostatic complexes of WPI-dextran conjugate and chondroitin sulfate. J Agric Food Chem 2016;64:5539-48.

Mishra R, Mir SR, Amin S. Polymeric nanoparticles for improved bioavailability of cilnidipine. Int J Pharm Pharm Sci 2017;9:120-39.

Arafat M. Approaches to achieve an oral controlled release drug delivery system using polymers. Int J Pharm Pharm Sci 2015;7:16-21.

Deore S, Shahi SR, Dabir P. Nanoparticle: as a targeted drug delivery system for depression. Int J Curr Pharm Res 2016;8:7-11.

Published

07-05-2020

How to Cite

RAHMAT, D., NURHIDAYATI, L., MARCELLA, M., SUMARNY, R., & LAKSMITAWATI, D. R. (2020). IN VITRO ANTI-PLATELET AGGREGATION ACTIVITY OF HYDROXYPROPYL CELLULOSE–CYSTEAMINE BASED NANOPARTICLES CONTAINING CRUDE BROMELAIN. International Journal of Applied Pharmaceutics, 12(3), 95–98. https://doi.org/10.22159/ijap.2020v12i3.35053

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