THE POTENTIAL OF CACAO (THEOBROMA CACAO L.) POD HUSK EXTRACT ON THE NUMBER OF FIBROBLAST CELLS OF WISTAR RATS WOUND POST-TOOTH EXTRACTION
DOI:
https://doi.org/10.22159/ijap.2019.v11s4.35285Keywords:
Cacao pods husk, Fibroblast, Tooth extractionAbstract
Objective: The aim of this study was to determine the effect of cacao (Theobroma cacao L.) pod husk extract on the number of fibroblast cells in rats’ wound post-tooth extraction.
Methods: A total of 24 rats were divided into 2 groups. Control group and a treatment group. The tooth extraction was performed on a mandibular left first molar. Cacao (Theobroma cacao L.) pod husk extract was applied on the treatment group, and a saline solution was applied on the control group once a day by intragastric administration. On the 3rd, 7th and 10th day, 4 rats’ were sacrificed and the mandibulars were subsequently removed for histological process. The fibroblast cells calculation was performed on the histological preparation using a microscope. The data were analyzed using One Way ANOVA.
Results: More fibroblast cells were observed in the treatment group when compared to the control group. The cacao (Theobroma cacao L.) pod husk extract administration for 10 d induced more fibroblast cell quantity compared to that for 3 d.
Conclusion: The cacao (Theobroma cacao L.) pod husk extract administration may enhance the fibroblast cell quantity in the rats with post-tooth extraction wounds.
Downloads
References
2. Velnar T, Bailey T, V Smrkloj. The wound healing process: an overview of the celluler and molecular mechanisms. Slovenia: J Int Med Res 2009;37:1528-2542.
3. Khullar, Shilpa. Healing of tooth extraction socket. Ghaziabad UP: Heal Talk 2012;4:37-9.
4. Tarigan, Rosina, Uke P. Moist wound healing. Mata Ajar Trend dan Issue dalam Keperawatan; 2007. p. 1-23.
5. Mendonça, Ricardo Jose de. Angiogenesis in wound healing. Brazil Department Biol Sci: Federal University Triangulo Mineiro 2009;2:259-67.
6. Pusat Penelitian Kopi dan Kakao Indonesia (PPKKI). Buku Pintar Budi Daya Kakao. Jakarta: Agro Media Pustaka; 2010. p. 47.
7. Soerjodibroto, Walujo. Dark chocolate healing. Jakarta: PT Elex Media Komputindo; 2008. p. 8.
8. Nabaatin, Isnadia. Penambahan ekstrak kulit buah kakao (theobroma cacao L.) pada periodontal dressing terhadap kepadatan kolagen luka gingiva kelinci. Jember: BMKGI 2014;3:28-38.
9. Prasetyo, Dani S. Potensi penambahan ekstrak kulit buah kakao (theobroma cacao L.) pada periodontal dressing terhadap jumlah sel makrofag pada luka gingiva kelinci (penelitian eksperimental laboratoris). Jember: Fakultas Kedokteran Gigi Universitas Jember; 2013. p. 7-15.
10. Izzudin, Faris A, Anisa N. The potential of cocoa (theobroma cacao L.) pods extract in periodontal dressing to rabbit gingival would healing. Scient Coorperations Med Workshop 2015;21:1-16.
11. Othman, Azizah, Amin I, Nawalyah AG, Ilham A. Antioxidant capacity and phenolic content of cocoa beans. Food Chem 2007;100:1425-530.
12. Robbins SL, Cotran RS, Kumar V. Buku ajar patologi robbins. 7th ed. Alih Bahasa oleh Awal Prasetyo, Brahm U dan Toni Priliono, Jakarta: EGC; 2007. p. 209-10.
13. Poeloengan, Masniari, Praptiwi. Uji aktivitas antibakteri ekstrak kulit buah manggis (garcinia mangostana Linn). Cibinong: Media Litbanng Kesehatan 2010;20:65-9.
14. Selmi, Carlo, Tin K Mao, Carl L Keen, Harold H, Schmitz dan M. Eric gershwin the anti-inflammatory properties of cocoa flavanols. J Cardiovasc Pharmacol 2006;47:163-71.
15. Siswanto A, Nurdiana D, Lisda H. Effect of haruan (channa striata) extract on fibroblast cells count in wound healing. J Dentomaxillofac Sci 2016;1:89-94.
Published
How to Cite
Issue
Section
