AMELIORATIVE EFFECT OF PHOENIX DACTYLIFERA ON ADVERSE EFFECTS OF LINEZOLID IN MALE ALBINO RATS
DOI:
https://doi.org/10.22159/ijap.2019.v11s5.T0023Keywords:
Phoenix dactylifera, Linezolid, Urea, Creatinine, Brain, Bone marrowAbstract
Objective: This study aimed to investigate the adverse effects of linezolid (LZD) on biochemical and hematological parameters and some organs
including bone marrow, brain, and kidneys and to investigate the possibility of methanolic extract of Phoenix dactylifera (MEPD) to counteract the
adverse effects of LZD.
Methods: Forty-eight adult male albino rats were allocated into four equal groups (each of 12 animals). The first group received Tween 80 orally. The
second group received was given 0.5 ml of LZD suspension 4% in Tween 80 (100 mg/kg body weight) orally. The third group received the same dose
of LZD suspension followed by 1 ml of MEPD orally. The rats in the last group were given 1 ml of MEPD (1000 mg/kg body weight) orally. Rats were
sacrificed and blood samples were collected for hematological and biochemical study. Femur bones, brain, and kidneys were dissected and kept in a
jar containing 10% formalin to perform the histopathological investigation.
Results: The dose of LZD administered for 14 successive days induced a mild-to-moderate hematological abnormalities including decrease in
hemoglobin content (7.88±0.18 g/L) on day-1 post-treatment. Significant increase in serum urea (59.75±0.85) and serum creatinine was observed
(1.89±0.04). On day-14 post-treatment, LZD induced mild-to-moderate cellular abnormalities in bone marrow, brain, and kidneys. The concurrent
oral administration of MEPD and LZD for the same period corrected the hematological, biochemical, and histopathological alterations induced by LZD.
Conclusion: It was concluded that MEPD clearly ameliorated these damaging effects induced by LZD.
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References
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of U-100592 and U-100766, two oxazolidinone antibacterial agents for
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action and in vitro and in vivo activities of S-6123, a new oxazolidinone
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Therapeutic effects of date fruits (Phoenix dactylifera) in the prevention
of diseases via modulation of anti-inflammatory, anti-oxidant and antitumour
activity. Int J Clin Exp Med 2014;7:483-91.
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stress and effect of treatment on the oxidation product decomposition
processes in IBD. Oxid Med Cell Longev 2018;2018:7918261.
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Schentag JJ. Linezolid for the treatment of multidrug-resistant, grampositive
infections: Experience from a compassionate-use program.
Clin Infect Dis 2003;36:159-68.
24. MacDougall C, Chambers HF. Protein synthesis inhibitors and
miscellaneous antibacterial agents. In: Brunton LL, Chabner BA,
Knollmann BC, editors. Goodman and Gilman’s The Pharmacological
Basis of Therapeutics. 12th ed. New York: McGraw Hill; 2011.
p. 1537-8.
25. Ufelle SA, Achukwu PU, Ghasi SI. Myelo-protective and
haematopoietic effects of seed extract fractions of Phoenix dactylifera
in wistar rats. Afr J Pharm Pharmacol 2016;10:936-44.
26. Kofteridis DP, Malliotakis P, Maraki S, Christofaki M, Samonis G.
Impact of prolonged treatment with linezolid on the human gut flora.
Int J Infect Dis 2009;13:e313-5.
27. French G. Safety and tolerability of linezolid. J Antimicrob Chemother
2003;51 Suppl 2:245-53.
28. Mozos I. Mechanisms linking red blood cell disorders and cardiovascular
diseases. Biomed Res Int 2015;2015:682054.
29. Natsumoto B, Yokota K, Omata F, Furukawa K. Risk factors for
linezolid-associated thrombocytopenia in adult patients. Infection
2014;42:1007-12.
30. Nayak S, Nandwani A, Rastogi A, Gupta V. Acute interstitial
nephritis and drug rash with secondary to linezolid. Indian J Nephrol
2012;22:367-9.
31. Al-Qarawi AA, Abdel-Rahman H, El-Mougy SA, Mousa HM,
Badreldin HA. Nephroprotective action of Phoenix dactylifera in
gentamicin-induced nephrotoxicity. Pharm Biol 2008;46:227-30.
32. Lin YH, Wu VC, Tsai IJ, Ho YL, Hwang JJ, Tsau YK, et al. High
frequency of linezolid-associated thrombocytopenia among patients
with renal insufficiency. Int J Antimicrob Agents 2006;28:345-51.
33. Onuh N, Ukaejiofo EO, Achukwu PU, Ufelle SA, Okwuosa CN,
Chukwuka CJ. Haemopoietic activity and effect of crude fruit extract
of Phoenix dactylifera on peripheral blood parameters. Int J Biol Med
Res 2012;3:1720-3.
34. Villani P, Regazzi MB, Marubbi F, Viale P, Pagani L, Cristini F, et al.
Cerebrospinal fluid linezolid concentrations in postneurosurgical central
nervous system infections. Antimicrob Agents Chemother
2002;46:936-7.
35. Bobylev I, Maru H, Joshi AR, Lehmann HC. Toxicity to sensory
neurons and Schwann cells in experimental linezolid-induced peripheral
neuropathy. J Antimicrob Chemother 2016;71:685-91.
36. Ahmed AF, Al-Qahtani JH, Al-Yousef HM, Al-Said MS, Ashour AE,
Al-Sohaibani M, et al. Proanthocyanidin-rich date seed extract
protects against chemically induced hepatorenal toxicity. J Med Food
2015;18:280-9.
Ulanowicz DA, Garmon SA, et al. Synthesis and antibacterial activity
of U-100592 and U-100766, two oxazolidinone antibacterial agents for
the potential treatment of multidrug-resistant gram-positive bacterial
infections. J Med Chem 1996;39:673-9.
2. Ford CW, Hamel JC, Wilson DM, Moerman JK, Stapert D,
Yancey RJ Jr., et al. In vivo activities of U-100592 and U-100766, novel
oxazolidinone antimicrobial agents, against experimental bacterial
infections. Antimicrob Agents Chemother 1996;40:1508-13.
3. Daly JS, Eliopoulos GM, Willey S, Moellering RC Jr. Mechanism of
action and in vitro and in vivo activities of S-6123, a new oxazolidinone
compound. Antimicrob Agents Chemother 1988;32:1341-6.
4. Swaney SM, Aoki H, Ganoza MC, Shinabarger DL. The oxazolidinone
linezolid inhibits initiation of protein synthesis in bacteria. Antimicrob
Agents Chemother 1998;42:3251-5.
5. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE,
Persing DH, et al. Interpreting chromosomal DNA restriction patterns
produced by pulsed-field gel electrophoresis: Criteria for bacterial
strain typing. J Clin Microbiol 1995;33:2233-9.
6. Gonzales RD, Schreckenberger PC, Graham MB, Kelkar S,
DenBesten K, Quinn JP. Infections due to vancomycin-resistant
Enterococcus faecium resistant to linezolid. Lancet 2001;357:1179.
7. Di Paolo A, Malacarne P, Guidotti E, Danesi R, Del Tacca M.
Pharmacological issues of linezolid: An updated critical review. Clin
Pharmacokinet 2010;49:439-47.
8. Beekmann SE, Gilbert DN, Polgreen PM; IDSA Emerging Infections
Network. Toxicity of extended courses of linezolid: Results of an
infectious diseases society of America emerging infections network
survey. Diagn Microbiol Infect Dis 2008;62:407-10.
9. Baliga MS, Bantwal R, Vittaldas B, Shaun MK, Harshith PB,
Praveen K, et al. A review of the chemistry and pharmacology of the
date fruits (Phoenix dactylifera L.). Food Res Int 2011;44:1812-22.
10. Mansouri A, Embarek G, Kokkalouc E, Kefalas P. Phenolic profile
and antioxidant activity of the Algerian ripe date palm fruit (Phoenix
dactylifera). Food Chem 2005;89:411-20.
11. Abedi A, Parviz M, Karimian SM, Rodsari S. The effect of aqueous
extract of Phoenix dactylifera pollen grain on sexual behavior of male
rats. J Phys Pharm Adv 2012;2:235-42.
12. Miller CJ, Dunn EV, Hashim IB. The glycaemic index of dates and
date/yoghurt mixed meals. Are dates ‘the candy that grows on trees’?
Eur J Clin Nutr 2003;57:427-30.
13. Patel MI, Makhija SJ. Toxicity assessment of linezolid and the
beneficial effects of human erythropoietin in mice. Eur J Exp Biol
2012;2:2172-81.
14. Agbon AN, Ingbian SN, Dahiru AU. Preliminary histological and
histochemical studies on the neuroprotective effect of aqueous fruit
extract of Phoenix dactylifera L. (Date Palm) on atesunate-induced
cerebellar damage in wistar rats. J Med 2014;1:204-9.
15. Arbiol-Roca A, Imperiali CE, Montserrat MM, Cerro AS, Bosch de
Basea AC, Navarro LS, et al. Reference intervals for a complete blood
count on an automated haematology analyser sysmex XN in healthy
adults from the Southern metropolitan area of Barcelona. EJIFCC
2018;29:48-54.
16. Patton C, Crouch G. Enzymatic colorimetric determination of urea.
Anal Chem 1977;49:464-9.
17. Henry TJ. Determination of serum creatinine. In: Clinical Chemistry:
Principles and Techniques. 2nd ed. New York: Harper and Row
Publishers; 1974.
18. Suvarnet S, Layton C, John BD. Bancroft’s Theory and Practice of
Histological Techniques. 7th ed. Philadelphia, PA: Elsevier; 2013.
19. Snedecor GW, Cochran WG. Statistical Methods. 8th ed. Iowa, USA:
Ames, Iowa State University Press; 1982.
20. Nagel S, Köhrmann M, Huttner HB, Storch-Hagenlocher B, Schwab S.
Linezolid-induced posterior reversible leukoencephalopathy syndrome.
Arch Neurol 2007;64:746-8.
21. Rahmani AH, Aly SM, Ali H, Babiker AY, Srikar S, Khan AA, et al.
Therapeutic effects of date fruits (Phoenix dactylifera) in the prevention
of diseases via modulation of anti-inflammatory, anti-oxidant and antitumour
activity. Int J Clin Exp Med 2014;7:483-91.
22. Dudzi?ska E, Gryzinska M, Ognik K, Gil-Kulik P, Kocki J. Oxidative
stress and effect of treatment on the oxidation product decomposition
processes in IBD. Oxid Med Cell Longev 2018;2018:7918261.
23. Birmingham MC, Rayner CR, Meagher AK, Flavin SM, Batts DH,
Schentag JJ. Linezolid for the treatment of multidrug-resistant, grampositive
infections: Experience from a compassionate-use program.
Clin Infect Dis 2003;36:159-68.
24. MacDougall C, Chambers HF. Protein synthesis inhibitors and
miscellaneous antibacterial agents. In: Brunton LL, Chabner BA,
Knollmann BC, editors. Goodman and Gilman’s The Pharmacological
Basis of Therapeutics. 12th ed. New York: McGraw Hill; 2011.
p. 1537-8.
25. Ufelle SA, Achukwu PU, Ghasi SI. Myelo-protective and
haematopoietic effects of seed extract fractions of Phoenix dactylifera
in wistar rats. Afr J Pharm Pharmacol 2016;10:936-44.
26. Kofteridis DP, Malliotakis P, Maraki S, Christofaki M, Samonis G.
Impact of prolonged treatment with linezolid on the human gut flora.
Int J Infect Dis 2009;13:e313-5.
27. French G. Safety and tolerability of linezolid. J Antimicrob Chemother
2003;51 Suppl 2:245-53.
28. Mozos I. Mechanisms linking red blood cell disorders and cardiovascular
diseases. Biomed Res Int 2015;2015:682054.
29. Natsumoto B, Yokota K, Omata F, Furukawa K. Risk factors for
linezolid-associated thrombocytopenia in adult patients. Infection
2014;42:1007-12.
30. Nayak S, Nandwani A, Rastogi A, Gupta V. Acute interstitial
nephritis and drug rash with secondary to linezolid. Indian J Nephrol
2012;22:367-9.
31. Al-Qarawi AA, Abdel-Rahman H, El-Mougy SA, Mousa HM,
Badreldin HA. Nephroprotective action of Phoenix dactylifera in
gentamicin-induced nephrotoxicity. Pharm Biol 2008;46:227-30.
32. Lin YH, Wu VC, Tsai IJ, Ho YL, Hwang JJ, Tsau YK, et al. High
frequency of linezolid-associated thrombocytopenia among patients
with renal insufficiency. Int J Antimicrob Agents 2006;28:345-51.
33. Onuh N, Ukaejiofo EO, Achukwu PU, Ufelle SA, Okwuosa CN,
Chukwuka CJ. Haemopoietic activity and effect of crude fruit extract
of Phoenix dactylifera on peripheral blood parameters. Int J Biol Med
Res 2012;3:1720-3.
34. Villani P, Regazzi MB, Marubbi F, Viale P, Pagani L, Cristini F, et al.
Cerebrospinal fluid linezolid concentrations in postneurosurgical central
nervous system infections. Antimicrob Agents Chemother
2002;46:936-7.
35. Bobylev I, Maru H, Joshi AR, Lehmann HC. Toxicity to sensory
neurons and Schwann cells in experimental linezolid-induced peripheral
neuropathy. J Antimicrob Chemother 2016;71:685-91.
36. Ahmed AF, Al-Qahtani JH, Al-Yousef HM, Al-Said MS, Ashour AE,
Al-Sohaibani M, et al. Proanthocyanidin-rich date seed extract
protects against chemically induced hepatorenal toxicity. J Med Food
2015;18:280-9.
Published
15-09-2019
How to Cite
ABDO SAID, M. A., AZIZ, S. A., & ELNABTITY, S. M. (2019). AMELIORATIVE EFFECT OF PHOENIX DACTYLIFERA ON ADVERSE EFFECTS OF LINEZOLID IN MALE ALBINO RATS. International Journal of Applied Pharmaceutics, 11(5), 9–13. https://doi.org/10.22159/ijap.2019.v11s5.T0023
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