OSTEOCALCIN GENE POLYMORPHISM IN INDONESIAN MEN WITH PERIODONTITIS
DOI:
https://doi.org/10.22159/ijap.2020.v12s1.ARL028Keywords:
Osteocalcin, Gene polymorphism, PeriodontitisAbstract
Objective: Through this work, we aimed to clarify the association between the severity of periodontitis and osteocalcin (OC) gene C298T polymorphism
in Indonesian male subjects.
Methods: DNA was extracted from blood serum samples of 100 consenting Indonesian men whose periodontitis was classified as mild, moderate,
or severe. Polymerase chain reaction and restriction fragment length polymorphism techniques were applied to evaluate the status of OC (C298T)
polymorphism using HindIII restriction enzyme and electrophoresis in agarose gel to separate the indicated fragments.
Results: Although genotype distribution of the OC C298T polymorphism showed a trend for more frequent occurrence of genotype hh (and allele h)
with increasing severity of periodontitis, this trend was not statistically significant in the tested sample population, in whom the HH genotype
appeared to be relatively rare.
Conclusion: The results showed no significant association between the severity of periodontitis and OC C298T polymorphism in Indonesian male
subjects.
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of Indonesia, editor. Households’ Health Survey (SKRT). Vol. 3.
Jakarta: Depkes; 2004. p. 130-47.
2. Tadjoedin FM, Fitri AH, Kuswandani SO, Sulijaya B, Soeroso Y. The
correlation between age and periodontal diseases. J Int Dent Med Res
2017;10:327-32.
3. Loe H, Morrison E. Epidemiology of periodontal disease. Contemp
Periodontics 2003;106-16.
4. Newman MG, Takei H, Klokkevold PR, Carranza FA. Carranza’s
Clinical Periodontology. Amsterdam, Netherlands: Elsevier; 2006.
p. 10.
5. Wiebe CB, Putnins EE. The periodontal disease classification system
of the American Academy of Periodontology--an update. J Can Dent
Assoc 2000;66:594-7.
6. Fedi PF, Vernino AR, Gray JL. The Periodontic Syllabus. Philadelphia,
PA: Lippincott Willians and Wilkins; 2000.
7. Hoang QQ, Sicheri F, Howard AJ, Yang DS. Bone recognition
mechanism of porcine osteocalcin from crystal structure. Nature
2003;425:977-80.
8. Dohi Y, Iki M, Ohgushi H, Gojo S, Tabata S, Kajita E, et al. A novel
polymorphism in the promoter region for the human osteocalcin
gene: The possibility of a correlation with bone mineral density in
postmenopausal Japanese women. J Bone Miner Res 1998;13:1633-9.
9. Lerner UH. Inflammation-induced bone remodeling in periodontal
disease and the influence of post-menopausal osteoporosis. J Dent Res
2006;85:596-607.
10. Ducy P, Desbois C, Boyce B, Pinero G, Story B, Dunstan C, et al.
Increased bone formation in osteocalcin-deficient mice. Nature
1996;382:448-52.
11. Auerkari EI, Suryandari DA, Umami SS, Kusdhany LS, Siregar TW,
Rahardjo TB, et al. Gene promoter polymorphism of RUNX2 and
risk of osteoporosis in postmenopausal Indonesian women. SAGE
Open Med 2014;2:2050312114531571. Available from: https://doi.
org/10.1177/2050312114531571
12. Auerkari E, Suhartono A, Djamal N, Verisqa F, Suryandari D,
Kusdhany L, et al. CRP and IL-1B gene polymorphisms and CRP in
blood in periodontal disease. Open Dent J 2013;7:88-93.
13. Livshits G. Quantitative genetics of circulating molecules associated
with bone metabolism: A review. J Musculoskelet Neuronal Interact
2006;6:47-61.
14. Deng HW, Recker RR. Gene mapping and identification for
osteoporosis. J Musculoskelet Neurol Interact 2004;4:91-100.
15. Deng HW, Shen H, Xu FH, Deng HY, Conway T, Zhang HT, et al.
Tests of linkage and/or association of genes for Vitamin D receptor,
osteocalcin, and parathyroid hormone with bone mineral density.
J Bone Miner Res 2002;17:678-86.
16. Xiao S, Huang Q, Kung AW. Genetics of osteoporosis in China. Int J
Rheum Dis 2008;11:359-65.
17. Lei SF, Zhang YY, Deng FY, Liu MY, Liu XH, Zhou XG, et al. Bone
mineral density and five prominent candidate genes in Chinese men:
Associations, interaction effects and their implications. Maturitas
2005;51:199-206.
18. Jiang DK, Xu FH, Liu MY, Chen XD, Li MX, Liu YJ, et al. No evidence
of association of the osteocalcin gene HindIII polymorphism with bone
mineral density in Chinese women. J Musculoskelet Neuronal Interact
2007;7:149-54.
19. Chen HY, Tsai HD, Chen WC, Wu JY, Tsai FJ, Tsai CH. Relation of
polymorphism in the promotor region for the human osteocalcin gene to
bone mineral density and occurrence of osteoporosis in postmenopausal
Chinese women in Taiwan. J Clin Lab Anal 2001;15:251-5.
20. Xu H, Xiao W, Luo D, Liu YM, Zou L, Kuang HB. Association
analysis of genetic polymorphisms and potential interaction of the
osteocalcin (BGP) and ER-alpha genes with body mass index (BMI) in
premenopausal Chinese women. Acta Pharmacol Sin 2010;31:455-60.
21. Lin GT, Tseng HF, Chang CK, Chuang LY, Liu CS, Yang CH, et al.
SNP combinations in chromosome-wide genes are associated with bone
mineral density in Taiwanese women. Chin J Physiol 2008;51:32-41.
22. Kim JG, Ku SY, Lee DO, Jee BC, Suh CS, Kim SH, et al. Relationship
of osteocalcin and matrix Gla protein gene polymorphisms to serum
osteocalcin levels and bone mineral density in postmenopausal Korean
women. Menopause 2006;13:467-73.
23. Zhang J, Sun X, Xiao L, Xie C, Xuan D, Luo G. Gene polymorphisms
and periodontitis. Periodontol 2000 2011;56:102-24.
Published
23-03-2020
How to Cite
KAULIKA, N., ALWASRIS, T., DJAMAL, N., SULIJAYA, B., TALBOT, C., & AUERKARI, E. I. (2020). OSTEOCALCIN GENE POLYMORPHISM IN INDONESIAN MEN WITH PERIODONTITIS. International Journal of Applied Pharmaceutics, 12(1), 18–20. https://doi.org/10.22159/ijap.2020.v12s1.ARL028
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