RESPONSE SURFACE METHODOLOGY APPLIED TO OXALIC ACID HYDROLYSIS OF OIL PALM EMPTY FRUIT BUNCH BIOMASS FOR D-XYLOSE PRODUCTION

Authors

  • HERMAN SURYAD Laboratory of Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia.
  • ARRY YANUAR Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia. E
  • HARMITA Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia.
  • PUTRI WINNY RACHMADANI Laboratory of Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF037

Keywords:

D-Xylose, Oxalic acid, Oil palm empty fruit bunch, Hydrolysate, Response surface methodology, Acid hydrolysis

Abstract

Objective: The study aimed to identify the best conditions using oxalic acid for hydrolysis of hemicellulose in oil palm empty fruit bunch (OPEFB)
biomass.
Methods: The analytical method of high-performance liquid chromatography (HPLC) was using a SUPELCOSIL LC-NH2 column, refractive index
detection detector, and three compositions of the mobile phase. At first, the hydrolysis of hemicellulose in OPEFB powder was optimized by applying
a response surface methodology. A three-variable, six-central composite design was used for the experiments. Temperature (between 95°C and
135°C), reaction time (between 10 and 110 min), and oxalic acid concentration (between 1% and 7% [w/v]) were evaluated by running 15 different
experiments at constant biomass concentrations. Then, hydrolysis was optimized again at the constant temperature selected with three variables:
OPEFB concentration, reaction time, and oxalic acid concentration. Hydrolysate samples were detoxified with carbon active, and furfural compound
was analyzed by gas chromatography with flame ionization detector.
Results: The optimum condition of HPLC was using acetonitrile: water (9:1) at a flow rate of 1.0 ml/min. The first hydrolysis results showed
a high yield of D-xylose produced, which was 6.40 g D-xylose/100 g OPEFB biomass, with a xylose recovery of 93.8%. However, this result was
not yet optimum. Further hydrolysis at constant temperature experiment produced the highest xylose yield of 13.13%, equivalent to 32 g/l
D-xylose.
Conclusion: The yield of D-xylose from mild hydrolysis using oxalic acid was similar to that using dilute sulfuric acid as used in the previous study
by Rahman et al.

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References

1. Guo H, Chang J, Yin Q, Wang P, Lu M, Wang X, et al. Effect of the
combined physical and chemical treatments with microbial fermentation
on corn straw degradation. Bioresour Technol 2013;148:361-5.
2. Rahman SH, Choudhury JP, Ahmad AL, Kamaruddin AH. Optimization
studies on acid hydrolysis of oil palm empty fruit bunch fiber for
production of xylose. Bioresour Technol 2007;98:554-9.
3. Sudiyani Y, Sembiring KC, Hendarsyah H, dan Alawiyah S. Alkaline
pretreatment and enzymatic saccharification of oil palm empty fruit
bunch fiber for ethanol production. Menara Perkebunan 2010;78:70-4.
4. Hayashi K. Environmental Impact of the Palm Oil Industry in Indonesia.
Proceeding of International Symposium on Eco Topia Science 2007,
No. ISETS07; 2007.
5. Suryadi H, Katsuragi T, Yoshida N, Suzuki S, Tani Y. Polyol production
by culture of methanol-utilizing yeast. J Biosci Bioeng 2000;89:236-40.
6. Zhang J, Geng A, Yao C, Lu Y, Li Q. Xylitol production from d-xylose
and horticultural waste hemicellulosic hydrolysate by a new isolate of
Candida athensensis sb18. Bioresour Technol 2012;105:134-41.
7. Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, et al.
Features of promising technologies for pretreatment of lignocellulosic
biomass. Bioresour Technol 2005;96:673-86.
8. Hollister M. Review: Lignin conversion by manganese peroxidase
(MnP). Enzyme Microbial Technol 2002;30:454-66.
9. Mosier NS, Sarikaya A, Ladisch CM, Ladisch MR. Characterization
of dicarboxylic acids for cellulose hydrolysis. Biotechnol Prog
2001;17:474-80.
10. Torget RW, Kadam KL, Hsu TA, Philippidis GP, Wyman CE.
Prehydrolysis Lignocellulose. Patent No. 5424217; 2002.
11. Lee JW, Rodrigues RC, Jeffries TW. Simultaneous saccharification and
ethanol fermentation of oxalic acid pretreated corncob assessed with
response surface methodology. Bioresour Technol 2009;100:6307-11.
12. Lee JW, Rodrigues RC, Kim HJ, Choi IG, Jeffries TW. The roles of
xylan and lignin in oxalic acid pretreated corncob during separate
enzymatic hydrolysis and ethanol fermentation. Bioresour Technol
2010;101:4379-85.
13. Kim HY, Lee JW, Jeffries TW, Choi IG. Response surface optimization
of oxalic acid pretreatment of yellow poplar (Liriodendron tulipifera)
for production of glucose and xylose monosaccarides. Bioresour
Technol 2011;102:1440-6.
14. Bradley N. The Response Surface Methodology. Indiana: Department
of Mathematical Sciences, Indiana University of South Bend; 2007.
15. Joseph JK, Joseph LG. Practical HPLC Method Development. 3rd ed.
New York: John Wiley and Sons Inc.;1997.
16. Rong C, Ding X, Zhu Y, Li Y, Wang L, Qu Y, et al. Production of
furfural from xylose at atmospheric pressure by dilute sulfuric acid and
inorganic salts. Carbohydr Res 2012;350:77-80.
17. Khuri AI, Mukhopadhyay S. Response Surface Methodology.
New York: John Wiley and Sons Inc.; 2010.
18. Roberto IC, Solange IM. Alternatives for Detoxification of Diluted-
Acid Lignocellulosic Hydrolyzates for Use in Fermentative Processes,
a Review. Lorena, Brazil: Department of Biotechnology, Faculty of
Chemical Engineering; 2003.
19. Kumar A, Singh LK, Ghosh S. Bioconversion of lignocellulosic
fraction of water-hyacinth (Eichhornia crassipes) hemicellulose
acid hydrolysate to ethanol by pichia stipitis. Bioresour Technol
2009;100:3293-7.
20. Delbecq F, Wang Y, Muralidhara A, El Ouardi K, Marlair G, Len C.
Hydrolysis of hemicellulose and derivatives-a review of recent
advances in the production of furfural. Front Chem 2018;6:146.
21. Tan HT, Dykes GA, Wu TY, Siow LF. Enhanced xylose recovery from
oil palm empty fruit bunch by efficient acid hydrolysis. Appl Biochem
Biotechnol 2013;170:1602-13.

Published

23-03-2020

How to Cite

SURYAD, H., YANUAR, A., HARMITA, & RACHMADANI, P. W. (2020). RESPONSE SURFACE METHODOLOGY APPLIED TO OXALIC ACID HYDROLYSIS OF OIL PALM EMPTY FRUIT BUNCH BIOMASS FOR D-XYLOSE PRODUCTION. International Journal of Applied Pharmaceutics, 12(1), 172–176. https://doi.org/10.22159/ijap.2020.v12s1.FF037

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