CONCENTRATION DEPENDENT EFFECTS OF CARBOXYMETHYL CHITOSAN ON DENTIN REMINERALIZATION WITH AMORPHOUS CALCIUM PHOSPHATE

Authors

  • HASTI DWI SETIATI Conservative Dentistry Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • ENDANG SUPRASTIWI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • DEWA AYU NYOMAN PUTRI ARTININGSIH Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • LUH PUTU TRISNA BUDI UTAMI Conservative Dentistry Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2020.v12s2.OP-42

Keywords:

Carboxymethyl chitosan, Amorphous calcium phosphate, Demineralized dentin, Non-collagenous protein analog

Abstract

Objective: Carboxymethyl chitosan (CMC) is a non-collagenous protein analog which has a similar role as dentin matrix protein 1. CMC stabilizes amorphous calcium phosphate (ACP); hence forming nanocomplexes of CMC-ACP. The purpose of this study was to evaluate the effect of CMC concentration in CMC-ACP on dentin remineralization.
Methods: Cavities were formed on the occlusal surfaces of freshly extracted premolar teeth. All samples were demineralized and immersed in phosphate-buffered saline and stored in a shaking incubator at 37°C. The teeth were randomly divided into five groups. Group 1 was control group (no treatment), whereas Groups 2, 3, 4, and 5 were treated with CMC-ACP containing 1%, 2.5%, 5%, and 10% CMC. The remineralized layer on the dentin surface was evaluated using scanning electron microscopy and energy-dispersive X-ray analysis.
Results: The highest dentin remineralization capacity was achieved in Group 5 (10% CMC), whereas diminishing effects were observed in Group 4 (5% CMC), Group 3 (2.5% CMC), and Group 2 (1% CMC). Although no significant differences in calcium levels were observed between 2.5%, 5%, and 10% CMC groups, phosphate levels differed significantly in all treatment groups.
Conclusion: Optimal dentin remineralization was achieved by the application of CMC–ACP containing 2.5% CMC.

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References

Cao CY, Mei ML, Li QL, Lo EC, Chu CH. Methods for biomimetic
remineralization of human dentine: A systematic review. Int J Mol Sci
2015;16:4615-27.
2. Chen Z, Cao S, Wang H, Li Y, Kishen A, Deng X, et al. Biomimetic
remineralization of demineralized dentine using scaffold of CMC/ACP
nanocomplexes in an in vitro tooth model of deep caries. PLoS One
2015;10:1-19.
3. Wang Y, Van Manh N, Wang H, Zhong X, Zhang X, Li C. Synergistic
intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on
a biomimetic strategy to promote the regeneration of bone defects. Int J
Nanomed 2016;11:2053-67.
4. Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ, Marshall GW Jr.
Biomechanical perspective on the remineralization of dentin. Caries
Res 2009;43:70-7.
5. Krogstad DV, Wang D, Lin-Gibson S. Polyaspartic acid concentration
controls the rate of calcium phosphate nanorod formation in high
concentration systems. Biomacromolecules 2017;18:3106-13.
6. Toledano M, Osorio R. New advanced materials for high performance
at the resin-dentine interface. Front Oral Biol 2015;17:39-48.
7. Han L, Okiji T. Bioactivity evaluation of three calcium silicate-based
endodontic materials. Int Endod J 2013;49:1-7.
8. Goldberg M. Dentin structure composition and mineralization. Front
Biosci 2011;E3:281.
9. Kalliola S, Repo E, Srivastava V, Heiskanen JP, Sirviö JA,
Liimatainen H, et al. The pH sensitive properties of carboxymethyl
chitosan nanoparticles cross-linked with calcium ions. Colloids Surf B
Biointerfaces 2017;153:229-36.
10. Fonseca-Santos B, Chorilli M. An overview of carboxymethyl
derivatives of chitosan: Their use as biomaterials and drug delivery
systems. Mater Sci Eng C 2017;77:1349-62.
11. Budiraharjo R, Neoh KG, Kang ET, Kishen A. Bioactivity of novel
carboxymethyl chitosan scaffold incorporating MTA in a tooth model.
Int Endod J 2010;43:930-9.

Published

24-07-2020

How to Cite

SETIATI, H. D., SUPRASTIWI, E., PUTRI ARTININGSIH, D. A. N., & BUDI UTAMI, L. P. T. (2020). CONCENTRATION DEPENDENT EFFECTS OF CARBOXYMETHYL CHITOSAN ON DENTIN REMINERALIZATION WITH AMORPHOUS CALCIUM PHOSPHATE. International Journal of Applied Pharmaceutics, 12(2), 31–33. https://doi.org/10.22159/ijap.2020.v12s2.OP-42

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