REMINERALIZATION EFFECT OF POLYASPARTIC ACID IN THE POLYMER-INDUCED LIQUID PRECURSOR PROCESS ON THE INTRAFIBRILLAR DENTIN (SCANNING ELECTRON MICROSCOPE AND ENERGY DISPERSIVE X-RAY ANALYSIS)
DOI:
https://doi.org/10.22159/ijap.2020.v12s2.OP-46Keywords:
Intrafibrillar remineralization, Non-collagenous protein analog, Polyaspartic acid, Polymer-induced liquid precursor, Demineralized dentinAbstract
Objective: This study aims to analyze remineralization that occurred in demineralized dentin following polyaspartic acid (pAsp) in PILP immersion.
Methods: Sixteen dentin block samples were immersed in demineralized solution. The samples were divided into controls (no pAsp immersion) or
pAsp immersion for 3, 7, or 14 days (n’s=4). The samples were evaluated using a scanning electron microscope (SEM) to observe morphology and
energy-dispersive X-ray (EDX) to observe calcium and phosphate levels.
Results: SEM revealed that pAsp immersion resulted in significantly higher dentin remineralization compared to the control group. However, there
were no significant differences among 3, 7, and 14 days of immersion. EDX revealed that remineralization occurred by the deposition of calcium and
phosphate ions.
Conclusion: Including pAsp in the PILP process produced rapid remineralization of demineralized dentin.
Downloads
References
Habelitz LE, Marshall JH, Marshall GW Jr. Biomechanical perspective
on the remineralization of dentin. Caries Res 2009;758:70-7.
Nudelman F, Lausch AJ, Sommerdijk NA, Sone ED. In vitro models of
collagen biomineralization. J Struct Biol 2013;183:258-69.
Xiao S, Liang K, Weir MD, Cheng L, Liu H, Zhou X, et al. Combining
bioactive multifunctional dental composite with PAMAM for root
dentin remineralization. Materials 2017;10:1-17.
Chen Z, Cao S, Wang H, Li Y, Kishen A, Deng X, et al. Biomimetic
remineralization of demineralized dentine using scaffold of CMC/ACP
nanocomplexes in an in vitro tooth model of deep caries. PLoS One
;10:1-19.
Nudelman F, Pieterse K, George A, Bomans PH, Friedrich H, Brylka LJ,
et al. The role of collagen in bone apatite formation in the presence of
hydroxyapatite nucleation inhibitors. Nat Mater 2010;9:9-14.
Jee S, Thula TT, Gower LB. Acta biomaterialia development of bonelike
composites via the polymer-induced liquid-precursor (PILP)
process. Part 1 : Influence of polymer molecular weight. Acta Biomater
;6:3676-86.
Gower LB. Biomimetic model systems for investigating the amorphous
precursor pathway and its role in biomineralization. Chem Rev
;108:4551-627.
Saeki K, Chien Y, Nonomura G, Chin AF, Habelitz S, Gower LB, et al.
Authors recovery after PILP remineralization of dentin lesions created
with two cariogenic acids. Arch Oral Biol 2017;82:194-202.
Krogstad DV, Wang D, Lin-gibson S. Polyaspartic acid concentration
controls the rate of calcium phosphate nanorod formation in high
concentration systems. Biomacromolecules 2017;18:3106-13.
Nurrohman H, Habelitz S, Marshall GW, Gower LB. Influence of
fluoride on the mineralization of collagen via the polymer-induced
liquid-precursor (PILP) process. Dent Mater 2018;34:1378-90.
Burwell AK, Thula-mata T, Gower LB, Habelitz S, Kurylo M, Ho SP,
et al. Functional remineralization of dentin lesions using polymerinduced
liquid-precursor process. PLoS One 2012;7:e38852.
Dai L, Liu Y, Salameh Z, Khan S, Mao J, Pashley DH, et al. Can
caries-affected dentin be completely remineralized by guided tissue
remineralization? Dent Hypotheses 2011;2:74-82.
Xu Y, Koen CH, Arno PM. Microscopic structure of the polymerinduced
liquid precursor for calcium carbonate microscopic structure
of the polymer-induced liquid precursor for calcium carbonate. Nat
Commun 2018;9:2582.
Bacino M, Girn V, Nurrohman H, Saeki K, Marshall SJ, Gower L,
et al. Integrating the PILP-mineralization process into a restorative
dental treatment. Dent Mater 2018;35:1-11.
Published
How to Cite
Issue
Section
