REMINERALIZATION EFFECT OF POLYASPARTIC ACID IN THE POLYMER-INDUCED LIQUID PRECURSOR PROCESS ON THE INTRAFIBRILLAR DENTIN (SCANNING ELECTRON MICROSCOPE AND ENERGY DISPERSIVE X-RAY ANALYSIS)

Authors

  • RIVANTI IRMADELA DEVINA Conservative Dentistry Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • ADELINE CLARISSA Conservative Dentistry Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • NILAKESUMA DJAUHARIE Department Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • KAMIZAR NAZAR Department Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • RATNA MEIDYAWATI Department Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2020.v12s2.OP-46

Keywords:

Intrafibrillar remineralization, Non-collagenous protein analog, Polyaspartic acid, Polymer-induced liquid precursor, Demineralized dentin

Abstract

Objective: This study aims to analyze remineralization that occurred in demineralized dentin following polyaspartic acid (pAsp) in PILP immersion.
Methods: Sixteen dentin block samples were immersed in demineralized solution. The samples were divided into controls (no pAsp immersion) or
pAsp immersion for 3, 7, or 14 days (n’s=4). The samples were evaluated using a scanning electron microscope (SEM) to observe morphology and
energy-dispersive X-ray (EDX) to observe calcium and phosphate levels.
Results: SEM revealed that pAsp immersion resulted in significantly higher dentin remineralization compared to the control group. However, there
were no significant differences among 3, 7, and 14 days of immersion. EDX revealed that remineralization occurred by the deposition of calcium and
phosphate ions.
Conclusion: Including pAsp in the PILP process produced rapid remineralization of demineralized dentin.

Downloads

Download data is not yet available.

References

Habelitz LE, Marshall JH, Marshall GW Jr. Biomechanical perspective

on the remineralization of dentin. Caries Res 2009;758:70-7.

Nudelman F, Lausch AJ, Sommerdijk NA, Sone ED. In vitro models of

collagen biomineralization. J Struct Biol 2013;183:258-69.

Xiao S, Liang K, Weir MD, Cheng L, Liu H, Zhou X, et al. Combining

bioactive multifunctional dental composite with PAMAM for root

dentin remineralization. Materials 2017;10:1-17.

Chen Z, Cao S, Wang H, Li Y, Kishen A, Deng X, et al. Biomimetic

remineralization of demineralized dentine using scaffold of CMC/ACP

nanocomplexes in an in vitro tooth model of deep caries. PLoS One

;10:1-19.

Nudelman F, Pieterse K, George A, Bomans PH, Friedrich H, Brylka LJ,

et al. The role of collagen in bone apatite formation in the presence of

hydroxyapatite nucleation inhibitors. Nat Mater 2010;9:9-14.

Jee S, Thula TT, Gower LB. Acta biomaterialia development of bonelike

composites via the polymer-induced liquid-precursor (PILP)

process. Part 1 : Influence of polymer molecular weight. Acta Biomater

;6:3676-86.

Gower LB. Biomimetic model systems for investigating the amorphous

precursor pathway and its role in biomineralization. Chem Rev

;108:4551-627.

Saeki K, Chien Y, Nonomura G, Chin AF, Habelitz S, Gower LB, et al.

Authors recovery after PILP remineralization of dentin lesions created

with two cariogenic acids. Arch Oral Biol 2017;82:194-202.

Krogstad DV, Wang D, Lin-gibson S. Polyaspartic acid concentration

controls the rate of calcium phosphate nanorod formation in high

concentration systems. Biomacromolecules 2017;18:3106-13.

Nurrohman H, Habelitz S, Marshall GW, Gower LB. Influence of

fluoride on the mineralization of collagen via the polymer-induced

liquid-precursor (PILP) process. Dent Mater 2018;34:1378-90.

Burwell AK, Thula-mata T, Gower LB, Habelitz S, Kurylo M, Ho SP,

et al. Functional remineralization of dentin lesions using polymerinduced

liquid-precursor process. PLoS One 2012;7:e38852.

Dai L, Liu Y, Salameh Z, Khan S, Mao J, Pashley DH, et al. Can

caries-affected dentin be completely remineralized by guided tissue

remineralization? Dent Hypotheses 2011;2:74-82.

Xu Y, Koen CH, Arno PM. Microscopic structure of the polymerinduced

liquid precursor for calcium carbonate microscopic structure

of the polymer-induced liquid precursor for calcium carbonate. Nat

Commun 2018;9:2582.

Bacino M, Girn V, Nurrohman H, Saeki K, Marshall SJ, Gower L,

et al. Integrating the PILP-mineralization process into a restorative

dental treatment. Dent Mater 2018;35:1-11.

Published

24-07-2020

How to Cite

DEVINA, R. I., CLARISSA, A., DJAUHARIE, N., NAZAR, K. ., & MEIDYAWATI, R. (2020). REMINERALIZATION EFFECT OF POLYASPARTIC ACID IN THE POLYMER-INDUCED LIQUID PRECURSOR PROCESS ON THE INTRAFIBRILLAR DENTIN (SCANNING ELECTRON MICROSCOPE AND ENERGY DISPERSIVE X-RAY ANALYSIS). International Journal of Applied Pharmaceutics, 12(2), 45–48. https://doi.org/10.22159/ijap.2020.v12s2.OP-46

Issue

Section

Original Article(s)