THE VIABILITY TEST OF SAPPAN WOOD (CAESALPINIA SAPPAN L.) ETHANOL EXTRACT IN THE H9C2 CELL LINE

IFA SULISTIYORINI; RATU SAFITRI; RONNY LESMANA; MAS RIZKY A. A. SYAMSUNARNO

doi:10.22159/ijap.2020.v12s3.39479

Authors

IFA SULISTIYORINI Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia
RATU SAFITRI Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia
RONNY LESMANA Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
MAS RIZKY A. A. SYAMSUNARNO Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia, 2Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s3.39479

Keywords:

H9C2 cell line, iron overload, Sappan wood ethanol extract

Abstract

Objective: In this study, the embryonic rat cardiomyocyte cell line H9C2 was used to investigate the cardiotoxicity effect of sappan wood ethanol extract (SWEE).

Methods: Sappan wood was extracted in 96% ethanol and divided into dose concentrations of 2.5, 5, 10, 50, 100, and 150 μg/ml, with deferiprone used as a control. Cell viability was assessed using the PrestoBlue Cell Viability Reagent, according to manufacturer protocols.

Results: Microscopic examination showed that the cell viability of H9C2 was preserved by SWEE treatments at a dose of 10 μg/ml and suggested dose concentrations of 50 μg/ml of SWEE. The percentage of viable cells was greater than 95% with a dose concentration of 10 μg/ml of SWEE, but it was significantly reduced with a dose concentration of 50 μg/ml of SWEE (p<0.05).

Conclusion: The optimal dose concentration of SWEE to reach 95% cell viability was 10 μg/ml.

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