EFFECT OF LYOPHILIZED JUICE OF PUNICA GRANATUM L. IN ANTINOCICEPTIVE AND INFLAMMATORY PAIN
DOI:
https://doi.org/10.22159/ijap.2021.v13s1.Y0055Keywords:
Antinociceptive, Lyophilized juice, Punica granatum L, Pomegranate, Formalin testAbstract
Objective: The aim of this study was to explore the analgesic activity of lyophilized juice of Punica granatum L., obtained from Hidalgo, Mexico, in the
formalin test.
Methods: We extracted the juice manually, filtered it and then dried down in a lyophilizer machine. We evaluated the antinociceptive effect of
lyophilized juice from pomegranate in the formalin test (2%) in male Wistar rats (180–200 g body weight). Thirty minutes before the test, a dose of
316 mg/kg (lyophilized juice) and acetylsalicylic acid as reference drug (100 mg/kg) both were administered intragastrically (i.g.).
Results: The oral administration of lyophilized juice of pomegranate showed a significant decrease in the number of flinches in the temporal course
and a significant antinociceptive effect in nociceptive and inflammatory pain compared with the vehicle. In the same way, this effect appeared with the
drug of reference (acetylsalicylic acid 100 mg/kg i.g). Furthermore, it was shown that juice had a 34% of antinociception on overall effect versus vehicle.
Conclusion: The results suggest that lyophilized juice of pomegranate has antinociceptive effect in nociceptive and inflammatory pain. Therefore, this
study supports the possible use of this lyophilized juice of pomegranate in the treatment of pain.
Downloads
Download data is not yet available.
References
1. Ong CK, Lirk P, Tan CH, Seymour RA. An evidence-based update on
non-steroidal anti-inflammatory drugs. Clin Med Res 2007;5:19-34.
2. Goldberg D, McGee S. Pain as a global public health priority. BMC
Public Health 2011;11:1-5.
3. da Rocha JL, de Tripodi Calamby RF, da Silva DF, Brandao HN,
Villarreal CF, de Lima FO. Evaluation of biological activity of Polygala
boliviensis in experimental models. Iran J Pharm Res 2019;18:793-802.
4. Newman D, Cragg G. Natural products as sources of new drugs over
the last 25 years. J Nat Prod 2007;70:461-77.
5. Mena P, Girones-Vilaplana A, Marti N, García-Viguera C. Pomegranate
varietal wines: Phytochemical composition and quality parameters.
Food Chem 2012;133:108-15.
6. Miguel MG, Neves MA, Maria DA. Pomegranate (Punica granatum L.):
A medicinal plant with myriad biological properties a short review. J
Med Plant Res 2010;4:2836-47.
7. González-Trujano ME, Pellicer F, Mena P, Moreno DA, García-
Viguera C. Antinociceptive and anti-inflammatory activities of a
pomegranate (Punica granatum L.) extract rich in ellagitannins. Int J
Food Sci Nutr 2015;66:395-9.
8. Labib RM, El-Ahmady SH. Antinociceptive, anti-gastric ulcerogenic
and anti-inflammatory activities of standardized Egyptian pomegranate
peel extract. J Appl Pharm Sci 2015;5:48-51.
9. Mansouri MT, Naghizadeh B, Ghorbanzadeh B. Ellagic acid enhances
the antinociceptive action of venlafaxine in mouse acetic acid-induced
pain: An isobolographic analysis. Pharmacol Rep 2015;67:473-7.
10. Ferreira LM, Sari MH, Cervi VF, Gehrcke M, Barbieri AV,
Zborowski VA, et al. pomegranate seed oil nanoemulsions improve the
photostability and in vivo antinociceptive effect of a non-steroidal antiinflammatory
drug. Colloids Surf B Biointerfaces 2016;144:214-21.
11. Zimmermann M. Ethical guidelines for investigation of experimental
pain in conscious animals. Pain 1983;16:109-10.
12. Nair VG, Praiapati PK, Nishteswar K, Unnikrishnan V, Nariva MB.
Analgesic and anti-inflammatory activities of Bulbophyllum
neilgherrense Wight. Pseudobulb: A folklore plant. Ayu 2018;39:76-80.
13. Gibaldi, M. Estimation of area under the curve. In: Gibaldi M, editor.
Biopharmaceutics and Clinical Pharmacokinetics. 4th ed. Philadelphia,
PA: Lea and Febiger; 1991. p. 377-8.
14. Meunier CJ, Burton J, Cumps J, Verbeeck RK. Evaluation of the
formalin test to assess the analgesic activity of diflunisal in the rat. Eur
J Pharm Sci 1998;6:311-6.
15. Rosland JH, Tjolsen A, Maehle B, Hole K. The formalin test in mice:
Effect of formalin concentration. Pain 1990;42:235-42.
16. Bozimowski G. A review of non-steroidal anti-inflammatory drugs.
AANA J 2015;83:425-33.
17. Malek Z, Dara SM, Jahromy MH. Antinociceptive effects of
pomegranate (Punica granatum L.) juice and seed extracts on acute
corneal pain in mice. World J Neurosci 2014; 4:99-105.
18. Sarker M, Das SC, Kumar-Saha S, Al Mahmud S, Bachar C. Analgesic
and anti-inflammatory activities of flower extracts of Punica granatum
Linn. (Punicaceae). J Appl Pharm Sci 2012;2:133-6.
19. Salwe KJ, Sachdev D. Evaluation of antinociceptive and antiinflammatory
effect of the hydroalcoholic extracts of leaves and fruit
peel of P. granatum in experimental animals. Asian J Pharm Clin Res
2014;7:137-41.
20. Larrosa M, González-Sarrías A, Yaañez-Gascón MJ, Selma MV,
Azorín-Ortuño M, Toti S, et al. Anti-inflammatory properties of a
pomegranate extract and its metabolite urolithin a in a colitis rat model
and the effect of colon inflammation on phenolic metabolism. J Nutr
Biochem 2010;21:717-25.
21. Noda Y, Kaneyuki T, Mori A, Packer L. Antioxidant activities of
pomegranate fruit extract and its anthocyanidins: Delphinidin,
cyanidin, and pelargonidin. J Agric Food Chem 2002;50:166-71.
22. Jurenka JS. Therapeutic applications of pomegranate (Punica
granatum L.): A review. Altern Med Rev 2008;13:128-44.
23. Ignarro LJ, Byrns RE, Sumi D, Nigris FD, Napoli C. Po megranate
juice protects nitric oxide against oxidative destruction and enhances
the biological actions of nitric oxide. Nitric Oxide 2006;15:93-102.
24. Vasantharaja D, Ramalingam V. Neurotoxic effect of titanium dioxide
nanoparticles: biochemical and pathological approach in male wistar
rats. Int J App Pharm 2018;19:74-81.
25. Hasan H, Oktawaty S, Kadang PM. Efficacy of pomegranate peel
extract, mangosteen peel extract, and combination of both extract on
incision wound healing in mice. Int J App Pharm 2019;11:5-7.
26. Toda N, Kishioka S, Hatano Y, Toda H. Modulation of opioid actions by
nitric oxide signaling (review). Anesthesiology 2009;1 Suppl 10:166-81.
non-steroidal anti-inflammatory drugs. Clin Med Res 2007;5:19-34.
2. Goldberg D, McGee S. Pain as a global public health priority. BMC
Public Health 2011;11:1-5.
3. da Rocha JL, de Tripodi Calamby RF, da Silva DF, Brandao HN,
Villarreal CF, de Lima FO. Evaluation of biological activity of Polygala
boliviensis in experimental models. Iran J Pharm Res 2019;18:793-802.
4. Newman D, Cragg G. Natural products as sources of new drugs over
the last 25 years. J Nat Prod 2007;70:461-77.
5. Mena P, Girones-Vilaplana A, Marti N, García-Viguera C. Pomegranate
varietal wines: Phytochemical composition and quality parameters.
Food Chem 2012;133:108-15.
6. Miguel MG, Neves MA, Maria DA. Pomegranate (Punica granatum L.):
A medicinal plant with myriad biological properties a short review. J
Med Plant Res 2010;4:2836-47.
7. González-Trujano ME, Pellicer F, Mena P, Moreno DA, García-
Viguera C. Antinociceptive and anti-inflammatory activities of a
pomegranate (Punica granatum L.) extract rich in ellagitannins. Int J
Food Sci Nutr 2015;66:395-9.
8. Labib RM, El-Ahmady SH. Antinociceptive, anti-gastric ulcerogenic
and anti-inflammatory activities of standardized Egyptian pomegranate
peel extract. J Appl Pharm Sci 2015;5:48-51.
9. Mansouri MT, Naghizadeh B, Ghorbanzadeh B. Ellagic acid enhances
the antinociceptive action of venlafaxine in mouse acetic acid-induced
pain: An isobolographic analysis. Pharmacol Rep 2015;67:473-7.
10. Ferreira LM, Sari MH, Cervi VF, Gehrcke M, Barbieri AV,
Zborowski VA, et al. pomegranate seed oil nanoemulsions improve the
photostability and in vivo antinociceptive effect of a non-steroidal antiinflammatory
drug. Colloids Surf B Biointerfaces 2016;144:214-21.
11. Zimmermann M. Ethical guidelines for investigation of experimental
pain in conscious animals. Pain 1983;16:109-10.
12. Nair VG, Praiapati PK, Nishteswar K, Unnikrishnan V, Nariva MB.
Analgesic and anti-inflammatory activities of Bulbophyllum
neilgherrense Wight. Pseudobulb: A folklore plant. Ayu 2018;39:76-80.
13. Gibaldi, M. Estimation of area under the curve. In: Gibaldi M, editor.
Biopharmaceutics and Clinical Pharmacokinetics. 4th ed. Philadelphia,
PA: Lea and Febiger; 1991. p. 377-8.
14. Meunier CJ, Burton J, Cumps J, Verbeeck RK. Evaluation of the
formalin test to assess the analgesic activity of diflunisal in the rat. Eur
J Pharm Sci 1998;6:311-6.
15. Rosland JH, Tjolsen A, Maehle B, Hole K. The formalin test in mice:
Effect of formalin concentration. Pain 1990;42:235-42.
16. Bozimowski G. A review of non-steroidal anti-inflammatory drugs.
AANA J 2015;83:425-33.
17. Malek Z, Dara SM, Jahromy MH. Antinociceptive effects of
pomegranate (Punica granatum L.) juice and seed extracts on acute
corneal pain in mice. World J Neurosci 2014; 4:99-105.
18. Sarker M, Das SC, Kumar-Saha S, Al Mahmud S, Bachar C. Analgesic
and anti-inflammatory activities of flower extracts of Punica granatum
Linn. (Punicaceae). J Appl Pharm Sci 2012;2:133-6.
19. Salwe KJ, Sachdev D. Evaluation of antinociceptive and antiinflammatory
effect of the hydroalcoholic extracts of leaves and fruit
peel of P. granatum in experimental animals. Asian J Pharm Clin Res
2014;7:137-41.
20. Larrosa M, González-Sarrías A, Yaañez-Gascón MJ, Selma MV,
Azorín-Ortuño M, Toti S, et al. Anti-inflammatory properties of a
pomegranate extract and its metabolite urolithin a in a colitis rat model
and the effect of colon inflammation on phenolic metabolism. J Nutr
Biochem 2010;21:717-25.
21. Noda Y, Kaneyuki T, Mori A, Packer L. Antioxidant activities of
pomegranate fruit extract and its anthocyanidins: Delphinidin,
cyanidin, and pelargonidin. J Agric Food Chem 2002;50:166-71.
22. Jurenka JS. Therapeutic applications of pomegranate (Punica
granatum L.): A review. Altern Med Rev 2008;13:128-44.
23. Ignarro LJ, Byrns RE, Sumi D, Nigris FD, Napoli C. Po megranate
juice protects nitric oxide against oxidative destruction and enhances
the biological actions of nitric oxide. Nitric Oxide 2006;15:93-102.
24. Vasantharaja D, Ramalingam V. Neurotoxic effect of titanium dioxide
nanoparticles: biochemical and pathological approach in male wistar
rats. Int J App Pharm 2018;19:74-81.
25. Hasan H, Oktawaty S, Kadang PM. Efficacy of pomegranate peel
extract, mangosteen peel extract, and combination of both extract on
incision wound healing in mice. Int J App Pharm 2019;11:5-7.
26. Toda N, Kishioka S, Hatano Y, Toda H. Modulation of opioid actions by
nitric oxide signaling (review). Anesthesiology 2009;1 Suppl 10:166-81.
Published
31-01-2021
How to Cite
GUERRERO-SOLANO, J. A., BAUTISTA, M., CRUZ-CANSINO, N. D. S., CHEHUE-ROMERO, A., & JARAMILLO-MORALES, O. A. (2021). EFFECT OF LYOPHILIZED JUICE OF PUNICA GRANATUM L. IN ANTINOCICEPTIVE AND INFLAMMATORY PAIN. International Journal of Applied Pharmaceutics, 13(1), 17–19. https://doi.org/10.22159/ijap.2021.v13s1.Y0055
Issue
Section
Full Proceeding Paper
