KLUTUK BANANA (MUSA BALBISIANA COLLA) PEEL FRACTIONS: ANTIOXIDANT AND ANTIHYPERGLYCEMIC POTENTIAL
DOI:
https://doi.org/10.22159/ijap.2021.v13s2.01Keywords:
Antihyperglycemic, Antioxidant, Musa balbisiana Colla, Klutuk banana peelAbstract
Objective: The purposes of this research were the antihyperglycemic and antioxidant determination of klutuk banana peel fractions through the oral glucose tolerance test (OGTT) and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods, respectively.
Methods: Klutuk banana peel was extracted by reflux method and followed by fractionation using n-hexane and ethyl acetate solvents by liquid-liquid extraction method. The statistical analysis used by one-way ANOVA and post hoc Tukey.
Results: The results of OGTT showed that ethyl acetate fraction of klutuk banana peel 14.5 mg/kg bw demonstrated higher activity to decrease blood glucose level compared to the other groups as much-32%,-49%,-47% and-40% at min 30, 60, 90 and 120, respectively. The IC50 of ethyl acetate fraction of klutuk banana peel was 0.3708 µg/ml, and its antioxidant activity index (AAI) 40.45.
Conclusion: The ethyl acetate fraction of klutuk peel presented very strong antioxidant and antihyperglycemic activity.
Downloads
Download data is not yet available.
References
1. American Diabetes Association. Classification and diagnosis of diabetes: standards of medical care in diabetes—2020. Diabetes Care 2020;43:14-31.
2. Bharti SK, Krishnan S, Kumar A, Kumar A. Antidiabetic phytoconstituents and their mode of action on metabolic pathways. Ther Adv Endocrinol Metab 2018;9:81-100.
3. Krinsky NI, Johnson EJ. Carotenoid actions and their relation to health and disease. Mol Aspects Med 2005;26:459-516.
4. Wall MM. Ascorbic acid, vitamin a, and mineral composition of banana (Musa sp.) and papaya (Carica papaya) cultivars grown in Hawaii. J Food Compos Anal 2006;19:434-45.
5. Singh B, Singh JP, Kaur A, Singh N. Bioactive compounds in banana and their associated health benefits-a review. Food Chem 2016;206:1-11.
6. Vijayakumar S, Presannakumar G, Vijayalakshmi NR. Antioxidant activity of banana flavonoids. Fitoterapia 2008;79:279-82.
7. Singh A, Singh S. Dietary fiber content of Indian diets. Asian J Pharm Clin Res 2015;8:58–61.
8. Navghare VV, Dhawale SC. In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels. Alexandria J Med 2017;53:237-43.
9. Nofianti T, Muhtadi A, Fidrianny I. Comparison of hypoglicemic effect of extract rinds, flesh, seeds and flowers Musa balbisiana colla in a mice. Abstract Proceeding 2nd Postgraduate Seminar on Pharmaceutical Sciences; 2019.
10. Meliala A, Sumiwi YAA, Narwidina P, Rini SLS, Setyaningsih W. Banana peel flakes alleviate blood glucose and stress in a dose-dependent manner. Int J Pharm Pharm Sci 2020;12:75-81.
11. Ministry of Health of the Republic of Indonesia. General Standard Parameters of Medicinal Plant Extracts. Jakarta: Directorate General of Food and Drug Control; 2000.
12. Borah M, Das S. Antidiabetic, antihyperlipidemic, and antioxidant activities of Musa balbisiana Colla. in type 1 diabetic rats. Indian J Pharmacol 2017;49:71–6.
13. Malpani MO, Rajput PR, Chinchole KV, Kapse SS, Ambarkar KS. Phytochemical screening and antioxidant activity of extracts of Xanthium strumarium, chrysanthemum and their mixture. Rasayan J Chem 2019;12:1901–8.
14. Ighodaro OM, Adeosun AM, Asejeje FO, Soetan GO, Kassim OO. Time course effects of 5,5-dihydroxylpyrimidine-2,4,6-trione (alloxan) as a diabetogenic agent in animal model. Alexandria Med J 2018;54:705-10.
15. Tchimene MK, Okoli CO, Iwu MM. Antidiabetic property of some Nigerian medicinal plants. J Med Plants Res 2016;10:139-48.
16. Oskouei BG, Ravasjani SA, Musavinejad SJ, Salehzadeh SA, Abdolhosseinzadeh A, Hamishehkar H, et al. In vivo evaluation of anti-hyperglycemic, anti-hyperlipidemic and anti-oxidant status of liver and kidney of thymol in STZ-induced diabetic rats. Drug Res (Stuttg) 2019;69:46-52.
17. Anal AK, Jaisanti S, Noomhorm A. Enhanced yield of phenolic extracts from banana peels (Musa acuminata Colla AAA) and cinnamon barks (Cinnamomum varum) and their antioxidative potentials in fish oil. J Food Sci Technol 2014;51:2632–9.
18. Kusuma SAF, Pebrianti M, Saraswati A. Comparison of unripe banana peel of kapok (Musa paradisiaca L.) and klutuk (Musa balbisiana Colla): phytochemical and anti-dysenteriae activity. J Pharm Sci Res 2018;10:911-4.
19. Toh PY, Leong FS, Chang SK, Khoo HE, Yim HS. Optimization of extraction parameters on the antioxidant properties of banana waste. Acta Sci Pol Technol Aliment 2016;15:65–78.
20. Martinez PDH, Merle J, Labidi J, Bouhtoury FCE. Tannins extraction: a key point for their valorization and cleaner production. J Clean Prod 2019;206:1138-55.
21. Mustarichie R, Runadi D, Ramdhani D. The antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea. BL. Dans). Asian J Pharm Clin Res 2017;10:343-7.
22. Thavamoney N, Sivanadian L, Tee LH, Khoo HE, Prasad KN, Kong KW. Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents. J Food Sci Technol 2018;55:2523–32.
23. Jiang Z, Kempinski C, Chappell J. Extraction and analysis of terpenes/terpenoids. Curr Protoc Plant Biol 2016;1:345–58.
24. Gonzalez MLC, Sepulveda L, Verma DK, Garcia HAL, Duran LVR, Llina A, et al. Review conventional and emerging extraction processes of flavonoids. Processes 2020;8:1-29.
25. Nerdy N, Manurung K. Spectrophotometric method for antioxidant activity test and total phenolic determination of red dragon fruit leaves and white dragon fruit leaves. Rasayan J Chem 2018;11:1183-92.
26. Sanchez NFS, Coronado RS, Canongo CV, Carlos BH. Chapter antioxidant compounds and their antioxidant mechanism. Intech Open 2019;1-28. DOI:10.5772/intechopen.85270
27. Zahoor M, Zafar R, Rahman NU. Isolation and identification of phenolic antioxidants from Pistacia integerrima gall and their anticholinesterase activities. Heliyon 2018;4:1-22.
28. Jan S, Khan MR, Rashid U, Bokhari J. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osong Public Health Res Perspect 2013;4:246-54.
29. Marjoni MR, Zulfisa A. Antioxidant activity of methanol extract/fractions of senggani leaves (Melastoma candidum D. Don). Pharm Anal Acta 2017;8:8:1-6.
30. Vargas RA, Zamilpa A, Petricevich VL. Development and validation of conditions for extracting flavonoids content and evaluation of antioxidant and cytoprotective activities from Bougainvillea x buttiana Bracteas (var. Rose). Antioxidants 2019;8:1-16.
31. Fidrianny I, Rizki K, Insanu M. In vitro antioxidant activities from various extracts of banana peels using ABTS, DPPH assays and correlation with phenolic, flavonoid, carotenoid content. Int J Pharm Pharm Sci 2014;6:299-303.
32. Setiawan NCE, Amalia H. Antioxidant activity of Areca vestiaria Giseke fruit seed extract and its fractions using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). J Cis-Trans (JC-T) 2017;1:9-13.
33. Handayani V, Ahmad AR, Sudir M. Antioxidant activity test of flower methanol extract and patikala leaf (Etlingera elatior (Jack) R. M. Sm) using the DPPH method. Pharm Sci Res 2014;1:86-93.
34. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 2010;4:118–26.
35. Félix R, Valentão P, Andrade PB, Felix C, Novais SC, Lemos MFL. Review evaluating the in vitro potential of natural extracts to protect lipids from oxidative damage. Antioxidants 2020;9:1-29.
36. Navghare VV, Dhawale SC, Phanse MA, Ingole PG, Pawale SS, Sonwane PP. Free radical scavenging property of some commonly known Musa species. Indo Am J Pharm Res 2013;3:6027–34.
37. Matsudha H, Morikawa T, Yoshikawa M. Antidiabetogenic constituents from several natural medicine. Pure Appl Chem 2002;74:1301-8.
38. Guy K, Jaekyung K, Klaus H, Yanyan C, Xiaozhuo C. Antidiabetes and anti-obesity activity of Laserstroemia speciose. Evid Complement Alternat Med 2007;44:401-7.
39. Pereira A, Maraschin M. Banana (Musa spp.) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health. J Ethnopharmacol 2015;160:149-63.
40. Anderson RA, Polansky MM. Tea enhances insulin activity. J Agric Food Chem 2002;50:7182-6.
41. Panche AN, Diwan AD, Chandra SR. Review article flavonoids: an overview. J Nutr Sci 2016;5:47, 1-15.
42. Saritha M. Flavonoids-the most potent poly-phenols as antidiabetic agents: an overview. Mod Appro Drug Des 2017;24:1-5.
43. Susilawati E. Antidiabetic activity of ethanol extract of hanjeli seed (Coix lacryma-Jobi) in alloxan-induced swiss webster mice. J Farm Galen 2017;2:77-82.
44. Alkhalidy H, Wang Y, Liu D. Dietary flavonoids in the prevention of T2D: an overview. Nutrients 2018;10:1-33.
45. Kondo S, Kittikorn M, Kanlayanarat S. Preharvest antioxidant activities of tropical fruit and the effect of low temperature storage on antioxidants and jasmonates. Postharvest Biol Technol 2005;36:309-18.
46. Ahmad BA, Zakariyya UA, Abubakar M, Sani MM, Ahmad MA. Chapter pharmacological activities of banana. Intech Open 2019;1-20. DOI:10.5772/intechopen.83299
2. Bharti SK, Krishnan S, Kumar A, Kumar A. Antidiabetic phytoconstituents and their mode of action on metabolic pathways. Ther Adv Endocrinol Metab 2018;9:81-100.
3. Krinsky NI, Johnson EJ. Carotenoid actions and their relation to health and disease. Mol Aspects Med 2005;26:459-516.
4. Wall MM. Ascorbic acid, vitamin a, and mineral composition of banana (Musa sp.) and papaya (Carica papaya) cultivars grown in Hawaii. J Food Compos Anal 2006;19:434-45.
5. Singh B, Singh JP, Kaur A, Singh N. Bioactive compounds in banana and their associated health benefits-a review. Food Chem 2016;206:1-11.
6. Vijayakumar S, Presannakumar G, Vijayalakshmi NR. Antioxidant activity of banana flavonoids. Fitoterapia 2008;79:279-82.
7. Singh A, Singh S. Dietary fiber content of Indian diets. Asian J Pharm Clin Res 2015;8:58–61.
8. Navghare VV, Dhawale SC. In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels. Alexandria J Med 2017;53:237-43.
9. Nofianti T, Muhtadi A, Fidrianny I. Comparison of hypoglicemic effect of extract rinds, flesh, seeds and flowers Musa balbisiana colla in a mice. Abstract Proceeding 2nd Postgraduate Seminar on Pharmaceutical Sciences; 2019.
10. Meliala A, Sumiwi YAA, Narwidina P, Rini SLS, Setyaningsih W. Banana peel flakes alleviate blood glucose and stress in a dose-dependent manner. Int J Pharm Pharm Sci 2020;12:75-81.
11. Ministry of Health of the Republic of Indonesia. General Standard Parameters of Medicinal Plant Extracts. Jakarta: Directorate General of Food and Drug Control; 2000.
12. Borah M, Das S. Antidiabetic, antihyperlipidemic, and antioxidant activities of Musa balbisiana Colla. in type 1 diabetic rats. Indian J Pharmacol 2017;49:71–6.
13. Malpani MO, Rajput PR, Chinchole KV, Kapse SS, Ambarkar KS. Phytochemical screening and antioxidant activity of extracts of Xanthium strumarium, chrysanthemum and their mixture. Rasayan J Chem 2019;12:1901–8.
14. Ighodaro OM, Adeosun AM, Asejeje FO, Soetan GO, Kassim OO. Time course effects of 5,5-dihydroxylpyrimidine-2,4,6-trione (alloxan) as a diabetogenic agent in animal model. Alexandria Med J 2018;54:705-10.
15. Tchimene MK, Okoli CO, Iwu MM. Antidiabetic property of some Nigerian medicinal plants. J Med Plants Res 2016;10:139-48.
16. Oskouei BG, Ravasjani SA, Musavinejad SJ, Salehzadeh SA, Abdolhosseinzadeh A, Hamishehkar H, et al. In vivo evaluation of anti-hyperglycemic, anti-hyperlipidemic and anti-oxidant status of liver and kidney of thymol in STZ-induced diabetic rats. Drug Res (Stuttg) 2019;69:46-52.
17. Anal AK, Jaisanti S, Noomhorm A. Enhanced yield of phenolic extracts from banana peels (Musa acuminata Colla AAA) and cinnamon barks (Cinnamomum varum) and their antioxidative potentials in fish oil. J Food Sci Technol 2014;51:2632–9.
18. Kusuma SAF, Pebrianti M, Saraswati A. Comparison of unripe banana peel of kapok (Musa paradisiaca L.) and klutuk (Musa balbisiana Colla): phytochemical and anti-dysenteriae activity. J Pharm Sci Res 2018;10:911-4.
19. Toh PY, Leong FS, Chang SK, Khoo HE, Yim HS. Optimization of extraction parameters on the antioxidant properties of banana waste. Acta Sci Pol Technol Aliment 2016;15:65–78.
20. Martinez PDH, Merle J, Labidi J, Bouhtoury FCE. Tannins extraction: a key point for their valorization and cleaner production. J Clean Prod 2019;206:1138-55.
21. Mustarichie R, Runadi D, Ramdhani D. The antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea. BL. Dans). Asian J Pharm Clin Res 2017;10:343-7.
22. Thavamoney N, Sivanadian L, Tee LH, Khoo HE, Prasad KN, Kong KW. Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents. J Food Sci Technol 2018;55:2523–32.
23. Jiang Z, Kempinski C, Chappell J. Extraction and analysis of terpenes/terpenoids. Curr Protoc Plant Biol 2016;1:345–58.
24. Gonzalez MLC, Sepulveda L, Verma DK, Garcia HAL, Duran LVR, Llina A, et al. Review conventional and emerging extraction processes of flavonoids. Processes 2020;8:1-29.
25. Nerdy N, Manurung K. Spectrophotometric method for antioxidant activity test and total phenolic determination of red dragon fruit leaves and white dragon fruit leaves. Rasayan J Chem 2018;11:1183-92.
26. Sanchez NFS, Coronado RS, Canongo CV, Carlos BH. Chapter antioxidant compounds and their antioxidant mechanism. Intech Open 2019;1-28. DOI:10.5772/intechopen.85270
27. Zahoor M, Zafar R, Rahman NU. Isolation and identification of phenolic antioxidants from Pistacia integerrima gall and their anticholinesterase activities. Heliyon 2018;4:1-22.
28. Jan S, Khan MR, Rashid U, Bokhari J. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osong Public Health Res Perspect 2013;4:246-54.
29. Marjoni MR, Zulfisa A. Antioxidant activity of methanol extract/fractions of senggani leaves (Melastoma candidum D. Don). Pharm Anal Acta 2017;8:8:1-6.
30. Vargas RA, Zamilpa A, Petricevich VL. Development and validation of conditions for extracting flavonoids content and evaluation of antioxidant and cytoprotective activities from Bougainvillea x buttiana Bracteas (var. Rose). Antioxidants 2019;8:1-16.
31. Fidrianny I, Rizki K, Insanu M. In vitro antioxidant activities from various extracts of banana peels using ABTS, DPPH assays and correlation with phenolic, flavonoid, carotenoid content. Int J Pharm Pharm Sci 2014;6:299-303.
32. Setiawan NCE, Amalia H. Antioxidant activity of Areca vestiaria Giseke fruit seed extract and its fractions using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). J Cis-Trans (JC-T) 2017;1:9-13.
33. Handayani V, Ahmad AR, Sudir M. Antioxidant activity test of flower methanol extract and patikala leaf (Etlingera elatior (Jack) R. M. Sm) using the DPPH method. Pharm Sci Res 2014;1:86-93.
34. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 2010;4:118–26.
35. Félix R, Valentão P, Andrade PB, Felix C, Novais SC, Lemos MFL. Review evaluating the in vitro potential of natural extracts to protect lipids from oxidative damage. Antioxidants 2020;9:1-29.
36. Navghare VV, Dhawale SC, Phanse MA, Ingole PG, Pawale SS, Sonwane PP. Free radical scavenging property of some commonly known Musa species. Indo Am J Pharm Res 2013;3:6027–34.
37. Matsudha H, Morikawa T, Yoshikawa M. Antidiabetogenic constituents from several natural medicine. Pure Appl Chem 2002;74:1301-8.
38. Guy K, Jaekyung K, Klaus H, Yanyan C, Xiaozhuo C. Antidiabetes and anti-obesity activity of Laserstroemia speciose. Evid Complement Alternat Med 2007;44:401-7.
39. Pereira A, Maraschin M. Banana (Musa spp.) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health. J Ethnopharmacol 2015;160:149-63.
40. Anderson RA, Polansky MM. Tea enhances insulin activity. J Agric Food Chem 2002;50:7182-6.
41. Panche AN, Diwan AD, Chandra SR. Review article flavonoids: an overview. J Nutr Sci 2016;5:47, 1-15.
42. Saritha M. Flavonoids-the most potent poly-phenols as antidiabetic agents: an overview. Mod Appro Drug Des 2017;24:1-5.
43. Susilawati E. Antidiabetic activity of ethanol extract of hanjeli seed (Coix lacryma-Jobi) in alloxan-induced swiss webster mice. J Farm Galen 2017;2:77-82.
44. Alkhalidy H, Wang Y, Liu D. Dietary flavonoids in the prevention of T2D: an overview. Nutrients 2018;10:1-33.
45. Kondo S, Kittikorn M, Kanlayanarat S. Preharvest antioxidant activities of tropical fruit and the effect of low temperature storage on antioxidants and jasmonates. Postharvest Biol Technol 2005;36:309-18.
46. Ahmad BA, Zakariyya UA, Abubakar M, Sani MM, Ahmad MA. Chapter pharmacological activities of banana. Intech Open 2019;1-20. DOI:10.5772/intechopen.83299
Published
10-02-2021
How to Cite
NOFIANTI, T., MUHTADI, A., FIDRIANNY, I., FUADAH, A. S., NURVIANA, V., & RUSWANTO. (2021). KLUTUK BANANA (MUSA BALBISIANA COLLA) PEEL FRACTIONS: ANTIOXIDANT AND ANTIHYPERGLYCEMIC POTENTIAL. International Journal of Applied Pharmaceutics, 13(2), 1–6. https://doi.org/10.22159/ijap.2021.v13s2.01
Issue
Section
Original Article(s)
