• LIUDMYLA SLOBODIANIUK Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001, Ternopil, Ukraine https://orcid.org/0000-0002-0400-1305
  • LILIIA BUDNIAK Department of Pharmacy Management, Economics and Technology, І. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001, Ternopil, Ukraine
  • SVITLANA MARCHYSHYN Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001, Ternopil, Ukraine https://orcid.org/0000-0001-9628-1350
  • OLHA SKRYNCHUK Department of Pharmacy, Bukovinian State Medical University, Theatralna sq. 2, 58002, Chernivtsi, Ukraine
  • VICTORIIA KUDRIA Department of Pharmacy, National Pirogov Memorial Medical University, Pirogov st. 56, 21000, Vinnytsya, Ukraine




Crambe cordifolia, Crambe koktebelica, Amino acids, HPLC, Leaves


Objective: The aim of our study was to establish the content of some primary metabolites, such as amino acids in Crambe cordifolia and Crambe koktebelica. The lack of experimental data induced us to determine these compounds.

Methods: Crambe cordifolia and Crambe koktebelica leaves were selected as the objects of the study. The amino acids in the raw materials were determined by the HPLC method.

Results: The results of the research revealed that the leaves of Crambe cordifolia and Crambe koktebelica contain fifteen and sixteen free amino acids respectively. Among the free amino acids L-histidine was presented in Crambe cordifolia leaves in the greatest amount, its content was 12.19 µg/mg. The content of free L-arginine, L-valine, L-phenylalanine, L-isoleucine was the greatest in Crambe koktebelica leaves, it was 2.23 µg/mg, 2.04 µg/mg, 1.74 µg/mg, 1.50 µg/mg respectively. The content of bound L-glutamic acid, Glycine, L-arginine, L-leucine was the highest in Crambe cordifolia and Crambe koktebelica leaves.

Conclusion: The results of the study showed that Crambe cordifolia and Crambe koktebelica can be considered as a source of highly digestible amino acids that can be used to treat some diseases.


Download data is not yet available.


Valarmathi R, Natarajan D. Gas chromatography and mass spectrometry analysis of bioactive compounds of Dryopteris hirtipes (Blumze) kuntze. Asian J Pharm Clin Res 2020;13:119-22.

Stoiko L, Kurylo K. Development of optimal technology of alcohol extract Centaurium erythraea rafn. Herb. Arch Balk Med Union 2018;53:523-8.

Darzuli N, Budniak L, Hroshovyi T. Selected excipients in oral solid dosage form with dry extract of Pyrola rotundifolia L. IJAP 2019;11:210-6.

Huzio N, Grytsyk A, Slobodianiuk L. Determination of carbohydrates in Agrimonia eupatoria L. herb. Sci Rise: Pharm Sci 2020;28:35-40.

Slobodianiuk L, Budniak L, Marchyshyn S, Basaraba R. Investigation of the hepatoprotective effect of the common cat’s foot herb dry extract. Pharmacologyonline 2020;3:310-8.

Saeedi R, Sultana A, Rahman K. Medicinal properties of different parts of Acacia nilotica linn (Babul), its phytoconstituents and diverse pharmacological activities. Int J Pharm Pharm Sci 2020;12:8-14.

Ahmed N, Mahmood A, Tahir SS, Bano A, Malik RN, Hassan S, et al. Ethnomedicinal knowledge and relative importance of indigenous medicinal plants of Cholistan desert, Punjab Province, Pakistan. J Ethnopharmacol 2014;155:1263-75.

Sevastre B, Sarpataki O, Stan RL, Taulescu M, Sevastre Berghian AC, Olah NK, et al. Anticancer activity of Euonymus europaeus fruits extract on human melanoma cells. Farmacia 2017;65:56-62.

Haidan Y, Qianqian M, Li Y, Guangchun P. The traditional medicine and modern medicine from natural products. Molecules 2016;21:559.

Kokila NR, Mahesh B, Mruthunjaya K. Exploration of bioactive components of Thunbergia coccinea, its pharmacognostic, antioxidant, GCMS and antihyperglycemic studies. Int J Pharm Pharm Sci 2020;12:45-54.

Kurylo K, Budniak L, Volska A, Zablotskyy B, Klishch I. Influence of phytocompositions on dynamics of changes in basal glycemia and glycemia in oral glucose tolerance test in rats with streptozotocin-nicotinamide-induced diabetes mellitus type 2. Georgian Med News 2020;300:112–6.

Francisco Ortega J, Fuertes Aguilar J, Gomez Campo C, Santos Guerra A, Jansen RK. Internal transcribed spacer sequence phylogeny of Crambe L. (Brassicaceae): molecular data reveal two old world disjunctions. Mol Phylogenet Evol 1999;11:361-80.

Prakhova TYa. New unconventional oilseed-abyssinian Crambe. J Bull ASAU 2013;8:8-10.

Pushkarova NO, Kalista MS, Kharkhota MA, Rakhmetov DB, Kuchuk MV. Biotechnological approaches for conservation of the endangered species Crambe koktebelica (Junge) N. Busch and effect of aseptic in vitro cultivation on its biochemical properties. Biotechnol Acta 2016;9:19-27.

Prina A. Taxonomic review of the genus "Crambe" sect. "Crambe" (Brassicaceae, Brassiceae). Ann Jard Bot Mad 2009;66:7-24.

Kalista MS. Underutilized medicinal species of Crambe L. of the flora of Ukraine. Agrobiodiversity for improving nutrition, health and life quality; 2017;1:216-20.

Bukhari SM, Simic N, Siddiqui HL, Ahmad VU. Determination of antioxidant activity of Crambe cordifolia. World Appl Sci J 2013;22:1561-5.

Lovatto NM, Goulart FR, Loureiro BB, Speroni CS, Bender ABB, Giacomini SJ, et al. Crambe (Crambe abyssinica) and sunflower (Helianthus annuus) protein concentrates: production methods and nutritional properties for use in fish feed. An Acad Bras Cienc 2017;89:2495-504.

Zorn K, Oroz Guinea I, Bornscheuer UT. Strategies for enriching erucic acid from Crambe abyssinica oil by improved Candida antarctica lipase a variants. Process Biochem 2019;79:65-73.

Razavi SM, Nejad Ebrahimi S. Chemical composition, allelopatic and cytotoxic effects of essential oils of flowering tops and leaves of Crambe orientalis L. from Iran. Nat Prod Res 2009;23:1492-8.

Aguinagalde I, Del Pero Martinez M. The occurrence of acylated flavonol glycosides in the cruciferae. Phytochemistry 1980;21:2875-8.

Rashid MA, Akhtar MN, Ashraf A, Nazir S, Ijaz A, Noradila AO, et al. Chemical composition and antioxidant, antimicrobial and haemolytic activities of Crambe cordifolia roots. Farmacia 2018;66:165-71.

Marchyshyn S, Slobodianiuk L, Budniak L, Skrynchuk O. Analysis of carboxylic acids of Crambe cordifolia steven. Pharmacia 2021;68:15-21.

Husak L, Dakhym I, Marchyshyn S, Nakonechna S. Determination of sugars and fructans content in Stachys sieboldii. IJGP 2018;12:70-4.

Budniak L, Slobodianiuk L, Marchyshyn S, Klepach P, Honcharuk Ya. Determination of carbohydrates content in Gentiana cruciata L. by GC/MS method. IJAP 2021;13:124-8.

Marchyshyn S, Budniak L, Slobodianiuk L, Ivasiuk I. Determination of carbohydrates and fructans content in Cyperus esculentus L. Pharmacia 2021;68:211-6.

Vons B, Tryhubchak O, Grochovuy T, Chubka M, Bihunyak V. Research of powders of the cryolyophilized xenoderm of porcine skin. IJGP 2018;12:657-64.

Slobodianiuk L, Budniak L, Marchyshyn S, Basaraba R. Determination of amino acids and sugars content in Antennaria dioica gaertn. IJAP 2019;11:39-43.

Hanczko R, Jambor A, Perl A, Molnar Perl I. Advances in the ophthalaldehyde derivatizations: comeback to the o-phthalaldehydeethanethiol reagent. J Chromatogr A 2007;1163:25-42.

Jambor A, Molnar Perl I. Amino acid analysis by high-performance liquid chromatography after derivatization with 9-fluorenylmethyloxycarbonyl chloride literature overview and further study. J Chromatogr A 2009;1216:3064-77.

Slobodianiuk L, Budniak L, Marchyshyn S, Sinichenko A, Demydiak O. Determination of amino acids of cultivated species of the genus Primula L. Biointerface Res Appl Chem 2021;11:8969-77.

Budniak L, Slobodianiuk L, Marchyshyn S, Demydiak O. Determination of Arnica foliosa nutt. fatty acids content by GC/MS method. Sci Rise: Pharm Sci 2020;28:14-8.

Budniak L, Slobodianiuk L, Marchyshyn S, Kostyshyn L, Horoshko O. Determination of composition of fatty acids in Saponaria officinalis L. Sci Rise: Pharm Sci 2021;29:25-30.

Ingle RA. Histidine biosynthesis. Arabidopsis Book; 2011.

Belitz HD, Grosch W, Schieberle P. Amino acids, peptides, proteins. In: Belitz HD, Grosch W, Schieberle P. editors. Food Chem. 2nd ed. Berlin: Springer-Verlag; 2009.

Moro J, Tome D, Schmidely P, Demersay TC, Azzout Marniche D. Histidine: a systematic review on metabolism and physiological effects in human and different animal species. Nutrients 2020;12:1414.

Kessler AT, Raja A. Biochemistry, histidine. In: Stat Pearls. Treasure Island (FL): StatPearls Publishing; 2020.

Holecek M. Histidine in health and disease: metabolism, physiological importance, and use as a supplement. Nutrients 2020;12:848.

Sudar Milovanovic E, Obradovic M, Jovanovic A. Benefits of L-arginine on cardiovascular system. Mini Rev Med Chem 2016;16:94-103.

Belitz HD, Grosch W, Schieberle P. Food chemistry. Berlin, Heidelberg: Springer; 2009.

Pahlavani N, Jafari M, Sadeghi O, Rezaei M, Rasad H, Rahdaret HA, et al. L-arginine supplementation and risk factors of cardiovascular diseases in healthy men: a double-blind randomized clinical trial. F1000Research 2017;3:306.

Kapalka GM. Practical resources for the mental health professional. Nutritional and herbal therapies for children and adolescents: A handbook for mental health clinicians. Elsevier Academic Press; 2010.

Liu Y, Xu Y, Ding D, Wen J, Zhu B, Zhang D. Genetic engineering of Escherichia coli to improve L-phenylalanine production. BMC Biotechnol 2018;18:5.

Ding D, Liu Y, Xu Y, Zheng P, Li H, Zhang D, et al. Improving the production of l-phenylalanine by identifying key enzymes through multi-enzyme reaction system in vitro. Sci Rep 2016;6:32208.

Sanchez S, Demain AL. Fermentation (industrial) | Production of amino acids. In: Batt CA, Tortorello ML. editors. Encyclopedia of Food Microbiology. 2nd ed. Academic Press; 2014.

Dutta S, Ray S, Nagarajan K. Glutamic acid as anticancer agent. Saudi Pharm J 2013;21:337-43.

Perez Torres I, Maria Zuniga Munoz A, Guarner Lans V. Beneficial effects of the amino acid glycine. Mini Rev Med Chem 2017;17:15-32.

Duan Y, Li F, Li Y, Tang Y, Kong X, Feng Z, et al. The role of leucine and its metabolites in protein and energy metabolism. Amino Acids 2016;48:41-51.



How to Cite

SLOBODIANIUK, L., BUDNIAK, L., MARCHYSHYN, S., SKRYNCHUK, O., & KUDRIA, V. (2021). HPLC ANALYSIS OF AMINO ACIDS CONTENT IN CRAMBE CORDIFOLIA AND CRAMBE KOKTEBELICA LEAVES. International Journal of Applied Pharmaceutics, 13(4), 111–116. https://doi.org/10.22159/ijap.2021v13i4.41265



Original Article(s)