NEW VALIDATED METHOD FOR THE ESTIMATION OF ALLANTOIN AND PERMETHRIN USIGN RP-HPLC IN BULK AND PHARMACEUTICAL DOSAGE FORM
Keywords:Allantoin, Permethrin, HPLC, Development, Validation
Objective: Special, effective high pressure liquid chromatography method has been developed for the simultaneous quantification of Allantoin and Permethrin.
Methods: By using Waters HPLC e-2695 quaternary pump with a PDA detector of 2998 instrument the chromatographic separation of Allantoin and Permethrin was achieved on the column of Symmetry C18 (150x4.6 mm, 3.5 µm) using an isocratic elution with a buffer containing 0.1percent ortho phosphoric acid and acetonitrile at a rate of 40:60 as a mobile phase with a flow rate of 1 ml/min at ambient temperature. A detector wavelength of 226 nm utilizing the PDA detector were given in the instrumental settings. The linearity was studied between the concentration range of 1-15 µg/ml of Allantoin and 25-375 µg/ml of Permethrin were injected with a run time of 6 min. As a part of method validation the parameters like specificity, linearity, accuracy, ruggedness, robustness were determined and the results were found to be within the allowable limit. Validation of the proposed method was carried out according to an International Conference on Harmonization (ICH) guidelines.
Results: LOD and LOQ for the two active ingredients were established with respect to test concentration. The plotted calibration curves were linear with a regression coefficient of R2>0.999, indicates that the linearity was with in the limit. As a part of method validation the parameters like specificity, linearity, accuracy, ruggedness, robustness were determined and the results were found to be within the allowable limit.
Conclusion: The method developed was found to be applicable to routine analysis and to be used for the measurement of both active pharmaceutical ingredients (i. e, Allantoin and Permethrin). Since, there is no HPLC method reported in the literature for the estimation of Allantoin and Permethrin, there is a need to develop quantitative methods under different conditions to achieve improvement in specificity, selecivity etc.
IP Simon, HS Huang, Hilda XH, Yu Jian Zhen. Effective Henry's law constants of glyoxal, glyoxylic acid, and glycolic acid. Geophys Res Lett 2009;36:L01802.
Eugene, Alexis J, Xia Sha-Sha, Guzman, Marcelo I. aqueous photochemistry of glyoxylic acid. J Phys Chem A 2016;120:3817–26.
Luo YC, Do JS, Liu CC. An amperometric uric acid biosensor based on modified Ir–C electrode. Biosens Bioelectron 2006;22:482–8.
Dahm R. Discovering DNA: friedrich miescher and the early years of nucleic acid research. Hum Genet 2008;122:565–81.
Pizzichini, Maria, Pandolfi, Maria Luisa, Arezzini, Laura, et al. Labelling of uric acid and allantoin in different purine organs and urine of the rat. Life Sci 1996;59:893–9.
Xi H, Schneider BL, Reitzer L. Purine catabolism in escherichia coli and function of xanthine dehydrogenase in purine salvage. J Bacteriol 2000;182:5332–41.
Johnson, Richard J, Sautin, Yuri Y, Oliver, William J, et al. Lessons from comparative physiology: could uric acid represent a physiologic alarm signal gone awry in western society? J Comp Physiol B 2009;179:67–76.
Riley P, Moore D, Ahmed F, Sharif MO, Worthington HV. Xylitol-containing products for preventing dental caries in children and adults. Cochrane Database Syst Rev 2015;26:CD010743.
Alshehri, Fahad Ali. The use of mouthwash containing essential oils (LISTERINE®) to improve oral health: a systematic review. Saudi Dent J 2018;30:2–6.
Kilian M, Chapple IL, Hannig M, Marsh PD, Meuric V, Pedersen AM, et al. The oral microbiome-an update for oral healthcare professionals. Br Dental J 2016;221:657–66.
Arowojolu AO, Gallo MF, Lopez LM, Grimes DA. Arowojolu AO. Combined oral contraceptive pills for treatment of acne. Cochrane Database Syst Rev 2012;11:CD004425.
Thornfeldt C. Cosmeceuticals containing herbs: fact, fiction, and future. Dermatol Surg 2005;31:873–80.
Pang B, McFaline JL, Burgis NE, Dong M, Taghizadeh K, Sullivan MR, et al. Defects in purine nucleotide metabolism lead to substantial incorporation of xanthine and hypoxanthine into DNA and RNA. Proc Natl Acad Sci USA 2012;109:2319–24.
Mamo Y, Seid E, Adams S, Gardiner A, Parry E. A primary healthcare approach to the management of chronic disease in Ethiopia: an example for other countries. Clin Med 2007;7:228–31.
Kandar R, Zakova P. Allantoin as a marker of oxidative stress in human erythrocytes. Clin Chem Lab Med 2008;46:1270–4.
Zitnanova I, Korytar P, Aruoma OI, Sustrova M, Garaiova I, et al. Uric acid and allantoin levels in down syndrome: antioxidant and oxidative stress mechanisms? Clin Chim Acta 2004;341:139–46.
Colin ME, Bonmatin JM, Moineau I. A method to quantify and analyze the foraging activity of honey bees: relevance to the sublethal effects induced by systemic insecticides. Arch Environ Contam Toxicol 2004;47:387–95.
Dressler C, Rosumeck S, Sunderkotter C, Werner RN, Nast A. The treatment of scabies. Deutsches Arzteblatt Int 2016;113:757–62.
Banks, Jonathan C, Paterson, Adrian M. A penguin-chewing louse (Insecta: Phthiraptera) phylogeny derived from morphology. Invertebr Syst 2004;18:89–100.
Erlanger. Field issues related to effectiveness of insecticide-treated nets in tanzania. Med Vet Entomol 2004;18:153–60.
Ujihara K. The history of extensive structural modifications of pyrethroids. J Pestic Sci 2019;44:215–24.
Bhavani P, Prasada Rao K, Mohan S. Novel validated reversed-phase high-performance liquid chromatography method for determination of glucosamine, diacerein, and methyl sulfonyl methane in micro sample rat plasma and its application to pharmacokinetic and dissolution studies. Asian J Pharm Clin Res 2020;13:50-63.
Palani Shanmugasundaram, Kamarapu SK. RP-HPLC method for the simultaneous estimation and validation of amlodipine besylate and atenolol in bulk and tablet dosage form in biorelevant dissolution medium (Fassif). Res J Pharm Tech 2017;10:3379-85.
Ashutosh Kumar S, Manidipa Debnath, Seshagiri Rao JVLN, Gowri Sankar D. Development and validation of a sensitive RP-HPLC method for simultaneous estimation of rosuvastatin and fenofibrate in tablet dosage form by using PDA detector in gradient mode. Res J Pharm Tech 2016;9:549-54.
Gomathy S, Narenderan ST, Meyyanathan SN, Gowramma B. Development and validation of hplc method for the simultaneous estimation of apigenin and luteolin in commercial formulation. J Crit Rev 2020;7:4785-90.
Malathi S, Devakumar D. Development and validation of rp-hplc method for the estimation of escitalopram oxalate and flupentixol dihydrochloride in combined dosage form and plasma. Int J Pharm Pharm Sci 2021;13:61-6.
Syed Rafi, Kantipudi Rambabu. Stability indicating validated HPLC method for the determination of aceclofenac and misoprostol in bulk and pharmaceutical formulation. Int J Res Pharm Sci 2020;11:7848-53.
Sruthi A, Uttam Prasad P. Stability indicating method development and validation of fimasartan by reverse phase high performance liquid chromatography in bulk and pharmaceutical dosage form. Asian J Pharm Clin Res 2021;14:138-46.
Vijayakumari M, Balasekhar Reddy Ch. Stability indicating validated hplc method for the determination of zanubrutinib in bulk and pharmaceutical dosage form. Asian J Pharm Clin Res 2020;13:159-62.
Mohinish Sahai, N Devanna. Validated stability indicating HPLC approach for quantifying tricholine citrate and cyproheptadine simultaneously in syrup forms. Int J Appl Pharm 2021;13:207-13.
Abdul Raziq, Syed Umer Jan. Relative comparison of stability and degradation of methylcobalamine tablets of different brands at different storage settings. Int J Appl Pharm 2021;13:171-5.
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