DEVELOPMENT AND PHARMACOKINETICS OF CURCUMIN LOADED BETA CAROTENE NANOPARTICLES IN RATS
DOI:
https://doi.org/10.22159/ijap.2021v13i5.42339Keywords:
Bio-availability, Curcumin, Beta carotene, Area under the curve, Overall elimination rate constantAbstract
Objective: The main objective of this research work is to improve the bioavailability of curcumin using beta carotene nanoparticles in experimental animals.
Methods: Curcumin-loaded beta carotene nanoparticles (NPs) have been prepared by emulsion–diffusion–evaporation method. Beta carotene and ethyl acetate were used to formulate NPs, and poly (vinyl alcohol) was used as a stabilizer. The emulsion diffusion evaporation method was used to prepare the nanoparticles and bioavailability and various pharmacokinetic parameters were compared with curcumin suspension in rats.
Results: The nanoparticles were successfully prepared using beta carotene and curcumin and the drug loading was almost 93% of the initial weight. Particle sizes were below 200 nm with negative zeta potentials. Comparing the pharmacokinetic parameters of curcumin loaded nanoparticles (Cmax, Tmax, Kel, and AUC were 105 µg/ml, 2 h, 0.23/h, and 1629 µg.hr/ml) with pure curcumin (Cmax, Tmax, Kel and AUC 16 µg/ml, 1h, 0.275/h and 58 µg.hr/ml) indicated a drastic improvement in bioavailability of curcumin.
Conclusion: The relative bioavailability was almost increased 30 times with beta carotene nanoparticles.
Downloads
References
Nayak AK, Ahmad SA, Beg S, Ara TJ, Hasnain MS. Drug delivery: present, past, and future of medicine. In: Inamuddin, Asiri AM, Mohammad A. (eds) Applications of nanocomposite materials in drug delivery. Woodhead Publishing Series in Biomaterials, Elsevier Inc; 2018. p. 255–82.
Ahmad R, Srivastava S, Ghosh S, Khare SK. Phytochemical delivery through nanocarriers: a review. Colloid Surface B 2021;197:111389.
Devadasu VR, Bhardwaj V, Kumar MR. Can controversial nanotechnology promise drug delivery? Chem Rev 2013;113:1686-735.
Marinoa T, Casellab P, Sangiorgioc P, Verardic A, Ferrarod A, Hristoforoud E, et al. Natural beta-carotene: a microalgae derivate for nutraceutical applications. Chem Eng 2020;79:1.
Sharifi Rad J, Rayess YE, Rizk AA, Sadaka C, Zgheib R, Zam W, et al. Turmeric and its major compound curcumin on health: bioactive effects and safety profiles for food, pharmaceutical, biotechnological and medicinal applications. Front Pharmacol 202;11:1021.
Devadasu VR, Wadsworth RM, Kumar MR. Protective effects of nanoparticulate coenzyme Q 10 and curcumin on inflammatory markers and lipid metabolism in streptozotocin-induced diabetic rats: a possible remedy to diabetic complications. Drug Delivery Transl Res 2011;1:448-55.
Shaikh J, Ankola DD, Beniwal V, Singh D, Kumar MR. Nanoparticle encapsulation improves oral bioavailability of curcumin by at least 9-fold when compared to curcumin administered with piperine as absorption enhancer. Eur J Pharm Sci 2009;37:223-30.
Rapalli VK, Kaul V, Gorantla S, Waghule T, Dubey SK, Pandey MM, et al. UV Spectrophotometric method for characterization of curcumin loaded nanostructured lipid nanocarriers in simulated conditions: method development, in vitro and ex-vivo applications in topical delivery. Spectrochim Acta Part A 2020;224:117392.
Lala RR, Shinde AS, Nandvikar NY. Solid lipid nanoparticles: a promising approach for combinational drug therapy in cancer. Int J Appl Pharm 2018;10:17-22.
Jogala SA, Chinnala KM, Aukunuru JI. Novel subcutaneous sustained release nanoparticles encapsulating low molecular weight heparin (LMWH): preparation, characterization and evaluation. Int J Pharm Pharm Sci 2016;8:264-8.
Gupta M. Synergistic anti-cancer effects of natural products and their mode of action. Asian J Pharm Clin Res 2021;14:15-21.
Shelat PR, Mandowara VK, Gupta DG, Patel SH. Formulation of curcuminoid loaded solid lipid nanoparticles in order to improve oral bioavailability. Int J Pharm Pharm Sci 2015;7:278-82.
Sapavatu SN, Chinthala R, Jadi RK. An overview on pharmacokinetics of polymeric nanoparticles intended for oral delivery. J Young Pharm 2020;12:187-9.
Published
How to Cite
Issue
Section
Copyright (c) 2021 RAVIKANTH CHINTHALA, SURYA SATYANARAYANA SINGH
This work is licensed under a Creative Commons Attribution 4.0 International License.