EFFECT OF AMIODARONE ON RABBITS' OPTIC NERVE AND THE AMELIORATIVE EFFECT OF VITAMIN E: FTIR STUDY

EMAN M. A.; SHERIF S. M.; SAHAR A. M.; AHLAM M. I.; GEHAN M. K.

doi:10.22159/ijap.2022v14i1.43507

Authors

EMAN M. A. Head of Biophysics and Laser Science Unit, Visual Science Department, Research Institute of Ophthalmology-Giza, Egypt https://orcid.org/0000-0003-0815-3063
SHERIF S. M. Head of Visual Science Department, Research Institute of Ophthalmology-Giza, Egypt https://orcid.org/0000-0002-6826-6011
SAHAR A. M. Assistant Lecturer, Physics Department, Faculty of Science (girls), Al Azhar University, Cairo, Egypt
AHLAM M. I. Physics Department, Faculty of Science (girls), Al Azhar University, Cairo, Egypt
GEHAN M. K. Physics Department, Faculty of Science (girls), Al Azhar University, Cairo, Egypt

DOI:

https://doi.org/10.22159/ijap.2022v14i1.43507

Keywords:

Amiodarone, Optic nerve, FTIR spectroscopy, Lipid, Protein, Antiarrhythmic drug, Rabbits, Vitamin E

Abstract

Objective: The study aimed to investigate the structural and conformational changes induced by short-term administration of the amiodarone in the optic nerve besides validating whether vitamin E coadministration with amiodarone will improve these changes.

Methods: Thirty New Zealand white rabbits from both sexes were haphazardly categorized into three groups, whereas each group contains ten rabbits (20 eyes). One of these groups served as a control that received an intraperitoneal injection of normal saline. Rabbits in the second group intraperitoneally (ip) injected daily with 160 mg/kg body weight (bw) of amiodarone for two weeks. The last group orally administration 100 mg/kg bw of vitamin E with the 160 mg/kg bw of amiodarone ip daily for two weeks until the time of sacrifice. Fourier transform infrared spectroscopy (FTIR) analysis was conducted on the optic nerve of the all groups.

Results: The results obtained from the FTIR spectrum revealed that the short-term administration of amiodarone caused a significant alteration in the stretching NH-OH region. A newly detected component centered at 3739±1 cm^-1 was assigned as _strO-H. There was a significant decrease (p˂0.05) in the bandwidth and band position of one component of _strO-H that centered at 3598±1 cm^-1. Moreover, remaining vibrational bands (O-H_asymand O-H_sym) were shifted to higher frequencies. Coadministration of vitamin E with amiodarone reduced the contour to four components as a control with significant increase in the band position of O-H_asym and the bandwidth of one component of _str O-H. Amiodarone administrations lead to reducing the area ratio of _asymCH₂ to _symCH₂and elevation of the area ratio of _asymCH₂ to _asymCH₃while the coadministration of vitamin E returned it as the control ratio. The percentage of the β-turn was significantly increased while the α-helix content was decreased due to amiodarone. The contents of both components were considered mimicking the control values when Vitamin E was co-administered with amiodarone.

Conclusion: The study stated that amiodarone could change the solubility and folding of the optic nerve proteins. Finally, vitamin E intake with amiodarone turns many of these changes induced by amiodarone to normal levels, which make it a good supplement for amiodarone users.

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