ANTICANCER ACTIVITY OF SILVER NANOPARTICLE OF PRODIGIOSIN ON LUNG CANCER
DOI:
https://doi.org/10.22159/ijap.2023v15i1.43628Keywords:
Prodigiosin, Lung cancer, Serratia marcenscens, Silver nanoparticlesAbstract
Objective: Nanotechnology is a relatively new branch of science and technology that studies and controls the interactions of synthetic and biological materials. Researchers are becoming more interested in nanoparticles as a result of their vast medicinal potential, particularly against cancer.
Methods: The Serratia marcescens culture supernatant containing Prodigiosin was used to synthesize silver nanoparticles in an environmentally benign biogenic manner. The effect of nanoparticles on the growth and proliferation of human lung cancer cell (A549) in vitro was investigated in this work. MTT Assay and DNA fragmentation assay were used to characterize the nanoparticles that had been produced.
Results: Cytotoxicity of the Prodigiosin AgNPs was represented as IC50 value of 31.2µg/ml and the viability decrease in the number of nanoparticles-treated cells. DNA fragmentation assay showed the degradation of DNA.
Conclusion: The present study conforms as the synthesized Prodigiosin AgNPs can be a promising anticancer agent regarding its mechanism of action.
Downloads
References
Feczko T, Arakha M, Fang JY, Yeh YC, Huang TH, Yang SC. Nano-based drug delivery or targeting to eradicate bacteria for infection mitigation: a review of recent advances. Front Chem 2020;1:286.
Vaidyanathan R, Kalishwaralal K, Gopalram S, Gurunathan S. Retracted: Nanosilver-the burgeoning therapeutic molecule and its green synthesis. Biotechnol Adv. 2009;27(6):924-37. doi: 10.1016/j.biotechadv.2009.08.001.
Malarkodi C, Rajeshkumar S, Paulkumar K, Vanaja M, Jobitha GDG, Annadurai G. Bactericidal activity of bio-mediated silver nanoparticles synthesized by Serratia nematodiphila. Drug Invent Today. 2013;5(2):119-25. doi: 10.1016/j.dit.2013.05.005.
He Y, Du Z, Ma S, Liu Y, Li D, Huang H. Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo. Int J Nanomedicine. 2016;11:1879-87. doi: 10.2147/IJN.S103695, PMID 27217750.
Zein R, Alghoraibi I, Soukkarieh C, Salman A, Alahmad A. In vitro anticancer activity against caco-2 cell line of colloidal nanosilver synthesized using aqueous extract of Eucalyptus camaldulensis leaves. Heliyon. 2020;6(8):04594. doi: 10.1016/j.heliyon.
El-Batal AI, El-Hendawy HH, Faraag AHI. In silico and in vitro cytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2). Bta. 2017;98(3):225-43. doi: 10.5114/bta.2017.70801.
Kavitha R, Aiswariya S, Ratnavali CMG. Anticancer activity of red pigment from Serratia marcescens in human cervix carcinoma. Int J PharmTech Res. 2010;2(1):784-7.
Boger DL, Patel M. Total synthesis of prodigiosin, prodigiosene, and desmethoxyprodigiosin: diels-alder reactions of heterocyclic azadienes and development of effective palladium(II)-promoted 2,2′-bipyrrole coupling procedure. J Org Chem. 1988;53(7):1405-15. doi: 10.1021/jo00242a013.
You Z, Liu X, Zhang S, Wang Y. Characterization of a prodigiosin synthetase PigC from Serratia marcescens jx-1 and its application in prodigiosin analogue synthesis. Biochem Eng J. 2018;134:1-11. doi: 10.1016/j.bej.2018.01.034.
Faraag AH, El-Batal AI, El-Hendawy HH. Characterization of prodigiosin produced by Serratia marcescens strain isolated from irrigation water in Egypt. Nat Sci. 2017;15(5):55-68.
Venil CK, Lakshmanaperumalsamy P. Application of statistical design to the optimization of culture medium for prodigiosin production by Serratia marcescens SWML08. Malays J Microbiol. 2009;5(1):55-61.
Marinkova D, Danalev D, Genchev G. Antimicrobial activity of Bulgarian products and their extracts containing bioactive components. J Chem Technol Metall. 2015;50(1):13-9.
Li Q, Ren FQ, Yang CL, Zhou LM, Liu YY, Xiao J. Anti-proliferation effects of isorhamnetin on lung cancer cells in vitro and in vivo. Asian Pac J Cancer Prev. 2015;16(7):3035-42. doi: 10.7314/apjcp.2015.16.7.3035, PMID 25854402.
Anburaj R, Jothiprakasam V. Biogenic potential of stabilized silver nanoparticles using G. sylvestrae and their biological assays. Int J Appl Pharm. 2019;11(3):130-7.
Sarangadharan S, Nallusamy S. Biosynthesis and characterization of silver nanoparticles produced by Bacillus licheniformis. Int J Pharm Med Biol Sci. 2015;4(4):236-9.
Nachiyar V, Sunkar S, Prakash P, Bavanilatha. Biological synthesis of gold nanoparticles using endophytic fungi. Pharm Chem. 2015;7(2):31-8.
Ruddaraju LK, Pallela PNVK, Pammi SVN, Padavala VS, Kolapalli VRM. Synergetic antibacterial and anticarcinogenic effects of Annona squamosa leaf extract mediated silver Nanoparticles. Mater Sci Semicond Process. 2019;100(Feb):301-9. doi: 10.1016/j.mssp.2019.05.007.
Rajasree SR, Suman TY. Extracellular biosynthesis of gold nanoparticles using a gram-negative bacterium Pseudomonas fluorescens. Asian Pac J Trop Dis. 2012;2Suppl 2:S796-9. doi: 10.1016/S2222-1808(12)60267-9.
Almasaudi SB, Al-Nahari AAM, Abd El-Ghany ESM, Barbour E, Al Muhayawi SM, Al-Jaouni S. Antimicrobial effect of different types of honey on Staphylococcus aureus. Saudi J Biol Sci. 2017;24(6):1255-61. doi: 10.1016/j.sjbs.2016.08.007. PMID 28855819.
Girma A, Seo W, She RC. Antibacterial activity of varying UMF-graded Manuka honeys. PLOS ONE. 2019;14(10):e0224495. doi: 10.1371/journal.pone.0224495. PMID 31652284.
Bhat MY, Gul MZ. MKH, Ghazi Aia. In vitro evaluation of antiproliferative, lipoxygenase and xanthine oxidase inhibitory activities of Artemisia nilagirica (C.B. Clarke) Pamp. Leaf extracts. Indian J Pharm Sci. 2019(Apr):389-95.
Priyadarshini S, Sonsudin F, Mainal A, Yahya R, Gopinath V, Vadivelu J. Phytosynthesis of biohybrid nano-silver anchors enhanced size-dependent photocatalytic, antibacterial, anticancer properties and cytocompatibility. Process Biochem. 2021;101(220):59-71. doi: 10.1016/j.procbio.2020.11.008.
Aderibigbe BA. Metal-based nanoparticles for the treatment of infectious diseases. Molecules. 2017;22(8). doi: 10.3390/molecules22081370, PMID 28820471.
Chaudhari PR, Masurkar SA, Shidore VB, Kamble SP. Effect of biosynthesized silver nanoparticles on staphylococcus aureus biofilm quenching and prevention of biofilm formation. Nano-Micro Lett. 2012;4(1):34-9. doi: 10.1007/BF03353689.
Saber MM, Mirtajani SB, Karimzadeh K. Green synthesis of silver nanoparticles using Trapa natans extract and their anticancer activity against A431 human skin cancer cells. J Drug Deliv Sci Technol. 2018;47:375-9. doi: 10.1016/j.jddst.2018.08.004.
Chandrappa CP, Chandrasekar N, Govindappa M, Shanbhag C, Singh UK, Masarghal J. Antibacterial activity of synthesized silver nanoparticles by simaroubaglauca against pathogenic bacteria. Int J Curr Pharm Sci. 2017;9(4):19-22. doi: 10.22159/ijcpr.2017v9i4.20629.
Govindaraju K, Tamilselvan S, Kiruthiga V, Singaravelu G. Biogenic silver nanoparticles by Solanum torvum and their promising antimicrobial activity. J Biopestic. 2010;3(1):394-9.
Singh T, Jyoti K, Patnaik A, Singh A, Chauhan R, Chandel SS. Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus. J Genet Eng Biotechnol. 2017;15(1):31-9. doi: 10.1016/j.jgeb.2017.04.005. PMID 30647639.
Rajeshkumar S, Malarkodi C, Venkat Kumar S. Synthesis and characterization of silver nanoparticles from marine brown seaweed and its antifungal efficiency against clinical fungal pathogens. Asian J Pharm Clin Res. 2017;10(2):190-3.
Anupama T, Amrish C. Antibacterial and anticancer potential of silver nanoparticles synthesized using gallic acid in betonite/stratch bio-nanocomposites. Int J Appl Pharm. 2018;10(5):178-89.
Published
How to Cite
Issue
Section
Copyright (c) 2023 Kavitha Rajendran, Shilpa KARNA, Dr.Damodharan N
This work is licensed under a Creative Commons Attribution 4.0 International License.
