BIOPHARMACEUTICAL STUDY OF BINARY POLOXAMER SYSTEMS AS IN SITU DRUG DELIVERY SYSTEMS POLOXAMER POLYCOMPLEXES: THE STUDY

ELENA O. BAKHRUSHINA; ELIZAVETA V. NOVOZHILOVA; ALENA S. KASHPERKO; ANNA V. SOKOLOVA; NATALIA B. DEMINA; IVAN I. KRASNYUK

doi:10.22159/ijap.2022v14i3.43930

Authors

ELENA O. BAKHRUSHINA Sechenov First State Medical University, 8 Trubetskaya Building 2, 119991, Moscow, Russia https://orcid.org/0000-0003-4351-360X
ELIZAVETA V. NOVOZHILOVA Sechenov First State Medical University, 8 Trubetskaya Building 2, 119991, Moscow, Russia https://orcid.org/0000-0002-1037-0617
ALENA S. KASHPERKO Sechenov First State Medical University, 8 Trubetskaya Building 2, 119991, Moscow, Russia https://orcid.org/0000-0002-9917-6772
ANNA V. SOKOLOVA Sechenov First State Medical University, 8 Trubetskaya Building 2, 119991, Moscow, Russia https://orcid.org/0000-0002-4961-704X
NATALIA B. DEMINA Sechenov First State Medical University, 8 Trubetskaya Building 2, 119991, Moscow, Russia https://orcid.org/0000-0003-4307-8791
IVAN I. KRASNYUK Sechenov First State Medical University, 8 Trubetskaya Building 2, 119991, Moscow, Russia

DOI:

https://doi.org/10.22159/ijap.2022v14i3.43930

Keywords:

In situ system, Polymer, Poloxamer, Mucoadhesion, Gelation temperature

Abstract

Objective: The article presents the results of studying the parameters of in situ systems based on poloxamers.

Methods: Natural, synthetic and semi-synthetic polymers were considered as additional gel-forming agents. The above-mentioned agents were cellulose derivatives, as well as rare-cross-linked acrylic polymers, alginic acid salts, and xanthan gum. The gelation temperature was visually recorded during the gradual heating. Mucoadhesion was determined by measuring the separation force of the sample from the mucosal model using pig stomach mucin. The concentration of gel formation was determined using an Ostwald capillary viscometer.

Results: During the experiment, 20 samples based on poloxamer 407 18% were examined with the addition of excipients in concentrations of 0.5-2.0%. It was found that the introduction of additional gel-forming agents has a positive effect on the mucoadhesive properties of poloxamer 407. To increase the temperature of gelation of the binary mixture, it is necessary to take into account the own concentration of gelation of the introduced additional polymer, which was confirmed by capillary viscometry. Also, additional viscometric studies revealed the reasons for the absence of a thermoreversible effect in the composition of a poloxamer with xanthan gum and confirmed its use as an excipient in the thermoreversible matrix of poloxamer 407 is impractical. Composition with the best-investigated biopharmaceutical properties-the composition with HEC in a concentration of 0.5%

Conclusion: The obtained results can be explained by the fact that when the concentration of additional polymer was exceeded, binary mixtures showed a decrease in the gel formation temperature up to a complete loss of the ability to temperature-dependent sol-gel transition. The choice of an additional gel-forming agent to improve the properties of the system should be based on some specific properties that affect the thermoreversible properties of the system.

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