• MEGHNA DABHADKAR SCES’s Indira College of Pharmacy, Mumbai Bangalore Highway, Tathawade, Pune, India 411033
  • MADHUR KULKARNI SCES’s Indira College of Pharmacy, Mumbai Bangalore Highway, Tathawade, Pune, India 411033



Dentin hypersensitivity, Thermoreversible sol-gel, Potassium nitrate, Poloxamer, Chitosan


Objective: The objective of the present study was to develop an in situ gelling formulation of potassium nitrate for the treatment of dentin hypersensitivity (DH).

Methods: Formulation was optimized using 32 full factorial design, wherein the concentration of two gelling agents, poloxamer 407 and chitosan (90% deacetylation) were the independent variables and formulation viscosity and gelation time were dependent variables. The trial formulations were evaluated for pH, spreadability, drug content, adherence time, and in vitro drug release, apart from viscosity and gelation time.

Results: The optimized formulation containing 22% of poloxamer 407 and 0.5 % of chitosan exhibited a gelation time of less than 150 s, a viscosity of about 2450 cps, and thermoreversible sol-gel transition behavior. The novel in situ gel showed a pH of 4.58, and spreadability as 5.55 g. cm/s and adherence time 4.3 h. The formulation could sustain the drug release over a period of 4 h and it was found to be 81.83±1.03%. Accelerated stability studies conducted over 3 mo duration ensured good physical and chemical stability of the formulation.

Conclusion: The optimized in situ gel formulation of potassium nitrate in the sol state is expected to allow easy and site-specific administration on the sensitive tooth. Upon application, the temperature-sensitive sol would get converted to a gel that would improve its retention time on the sensitive tooth and extend the duration of therapeutic action by sustaining the drug release.


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Neelakantan P. Desensitizing toothpastes for treatment of dentin hypersensitivity. Int J PharmTech Res. 2013 Dec;5(4):1769-73.

Miglani S, Aggarwal V, Ahuja B. Dentin hypersensitivity: recent trends in management. J Conserv Dent. 2010 Oct;13(4):218-24. doi: 10.4103/0972-0707.73385, PMID 21217949.

Chowdhary Z, Gupta P, Kaur J, Garg Y, Swarup N. Multifaceted assessment of dentine hypersensitivity, evaluation of demographic prevalence along with associated factors: A cross-sectional study. J Indian Soc Periodontol. 2019 Feb;23(1):64-8. doi: 10.4103/jisp.jisp_425_18, PMID 30692746.

Bubteina N, Garoushi S. Dentine hypersensitivity: a review. Dentistry. 2015 Jan;5:330.

Porto ICCM, Andrade AKM, Montes MAJR. Diagnosis and treatment of dentinal hypersensitivity. J Oral Sci. 2009 Sep;51(3):323-32. doi: 10.2334/josnusd.51.323, PMID 19776498.

Orchardson R, Gillam DG. The efficacy of potassium salts as agents for treating dentin hypersensitivity. J Orofac Pain. 2000;14(1):9-19. PMID 11203743.

Goswami L, Preeti Kothiyal NB. In situ gel: A study of dental diseases. J Appl Pharm Res. 2014 Jun;2(3):10-7.

Yadav R, Kanwar IL, Haider T, Pandey V, Gour V, Soni V. In situ gel drug delivery system for periodontitis: an insight review. Futur J Pharm Sci. 2020 Dec;6(1):33. doi: 10.1186/s43094-020-00053-x.

Cho HJ, Balakrishnan P, Park EK, Song KW, Hong SS, Jang TY. Poloxamer/cyclodextrin/chitosan-based thermoreversible gel for intranasal delivery of fexofenadine hydrochloride. J Pharm Sci. 2011 Feb;100(2):681-91. doi: 10.1002/jps.22314, PMID 20803575.

Bhowmik M, Kumari P, Sarkar G, Bain MK, Bhowmick B, Mollick MdMR, Mondal D, Maity D, Rana D, Bhattacharjee D, Chattopadhyay D. Effect of xanthan gum and guar gum on in situ gelling ophthalmic drug delivery system based on poloxamer-407. Int J Biol Macromol. 2013 Nov;62:117–23.

Gratieri T, Gelfuso GM, Rocha EM, Sarmento VH, de Freitas O, Lopez RFV. A poloxamer/chitosan in situ forming gel with prolonged retention time for ocular delivery. Eur J Pharm Biopharm. 2010 Jun;75(2):186-93. doi: 10.1016/ j.ejpb.2010.02.011, PMID 20188828.

Khanam N, Alam MI, Md Yusuf Ali QMAI, Siddiqui A. A review on optimization of drug delivery system with experimental designs. Int J Appl Pharm. 2018 Mar;10(2):7-12. doi: 10.22159/ijap.2018v10i2.24482.

Miller SC, Donovan MD. Effect of poloxamer 407 gel on the miotic activity of pilocarpine nitrate in rabbits. International Journal of Pharmaceutics. 1982 Oct;12(2-3):147-52. doi: 10.1016/0378-5173(82)90114-4.

Swain GP, Patel S, Gandhi J, Shah P. Development of moxifloxacin hydrochloride loaded in situ gel for the treatment of periodontitis: In vitro drug release study and antibacterial activity. J Oral Biol Craniofac Res. 2019 Jul;9(3):190-200. doi: 10.1016/j.jobcr.2019.04.001, PMID 31080718.

Padmasri B, Nagaraju R, Prasanth D. A comprehensive review on in situ gels. Int J App Pharm. 2020 Sep 16:24-33. doi: 10.22159/ijap.2020v12i6.38918.

Bartold PM. Dentinal hypersensitivity: a review. Aust Dent J. 2006 Sep;51(3):212-8. doi: 10.1111/j.1834-7819.2006.tb00431.x, PMID 17037886.

Erdemir U, Saygi G, Yucel T, Yildiz E. Dentin hypersensitivity and recent developments in treatment options: A mini-review. JSM Dent. 2016 Oct;4(4):1072.

Pereira R, Chava VK. Efficacy of a 3% potassium nitrate desensitizing mouthwash in the treatment of dentinal hypersensitivity. J Periodontol. 2001 Dec;72(12):1720-5. doi: 10.1902/jop.2001.72.12.1720, PMID 11811508.

Sharma S, Shetty NJ, Uppoor A. Evaluation of the clinical efficacy of potassium nitrate desensitizing mouthwash and toothpaste in the treatment of dentinal hypersensitivity. J Clin Exp Dent. 2012 Feb;4(1):e28-33. doi: 10.4317/jced.50665, PMID 24558521.

Hsu HC, Lee SS, Chang YC. Clinical efficacy of toothpaste containing 8.0% arginine and calcium carbonate for teeth hypersensitivity. J Dent Sci. 2013 Dec;8(4):444-7. doi: 10.1016/j.jds.2013.06.008.

Davari A, Ataei E, Assarzadeh H. Dentin hypersensitivity: etiology, diagnosis and treatment; a literature review. J Dent (Shiraz). 2013 Sep;14(3):136-45. PMID 24724135.

Kouchak M. In situ gelling systems for drug delivery. Jundishapur J Nat Pharm Prod. 2014 Aug;9(3):e20126. doi: 10.17795/jjnpp-20126, PMID 25237648.

Gadad AP, Wadklar PD, Dandghi P, Patil A. Thermosensitive in situ gel for ocular delivery of lomefloxacin. IJPER. 2016 Apr;50(2):S96-105.

Sarada K. In situ gelling system: a review. Int J Curr Pharm Rev Res. 2015 Jan;5(4):56-69.

Singh K, Hari Kumar SL. Injectable in situ gelling controlled release drug delivery system. Int J Drug Dev Res. 2012 Mar;4(2):56-69.

Dumortier G, Grossiord JL, Agnely F, Chaumeil JC. A review of poloxamer 407 pharmaceutical and pharmacological characteristics. Pharm Res. 2006 Jan;23(12):2709-28. doi: 10.1007/s11095-006-9104-4, PMID 17096184.

Chen J, Zhou R, Li L, Li B, Zhang X, Su J. Mechanical, rheological and release behaviors of a poloxamer 407/Poloxamer 188/Carbopol 940 thermosensitive composite hydrogel. Molecules. 2013 Oct 8;18(10):12415-25. doi: 10.3390/molecules181012415, PMID 24108402.

Fakhari A, Corcoran M, Schwarz A. Thermogelling properties of purified poloxamer 407. Heliyon. 2017 Aug;3(8):e00390. doi: 10.1016/j.heliyon.2017.e00390, PMID 28920092.

Waly Gihan H. Preparation and characterization of chitosan-based post-bleaching enamel remineralizing gel. Acta Sci Dent Sci. 2018;2:6.

Goy RC, Morais STB, Assis OBG. Evaluation of the antimicrobial activity of chitosan and its quaternized derivative on E. coli and S. aureus growth. Revista Brasileira de Farmacognosia. 2016 Jan;26(1):122-7. doi: 10.1016/j.bjp.2015.09.010.

Popa L, Ghica MV, Pirvu CD. Periodontal chitosan gels designed for improved local intra-pocket drug delivery. Farmacia. 2016 Mar;61:240-50.

Nayak PA, Nayak UA, Khandelwal V. The effect of xylitol on dental caries and oral flora. Clin Cosmet Investig Dent. 2014;6:89-94. doi: 10.2147/CCIDE.S55761, PMID 25422590.

Aishwarya, Harini N, Karthikeyan M. Clove oil and its role in oral health-a review. Int J Pharm Sci Health Care. 2014 Jun;4(3):155-69.

Srivastava R, Srivastava S, Singh SP. Thermoreversible in situ nasal gel formulations and their pharmaceutical evaluation for the treatment of allergic rhinitis containing extracts of Moringa olifera and embelia ribes. Int J Appl Pharm. 2017 Nov;9(6):16-20. doi: 10.22159/ijap.2017v9i6.18780.

Weissman SA, Anderson NG. Design of experiments (DoE) and process optimization. A review of recent publications. Org Process Res Dev. 2015 Nov;19(11):1605-33. doi: 10.1021/op500169m.



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