ISONIAZID-BASED SCHIFF'S BASES IN BONE CANCER STUDIES USING MG-63 CELL LINES
DOI:
https://doi.org/10.22159/ijap.2022.v14ti.48Keywords:
Isoniazid, Inhibitary zone, MG63 cell lines, Carcinoma, Osteosarcoma cells, Cytotoxicity, Apoptosis assay, Dual stainingAbstract
Objective: Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed and described using an in vitro evaluation employing cytotoxicity and apoptosis assay.
Methods: MG-63 cells were used in an MTT assay to examine the effect of the compound on cell viability (S1 and S2). Cell morphologies and IC50 values were obtained. The acridine orange (AO)/ethidium bromide (EB) dual staining technique was used to determine the apoptosis process.
Results: Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer.
Conclusion: Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer.
Downloads
References
Liu K, Liu PC, Liu R, Wu X. Dual AO/EB staining to detect apoptosis in osteosarcoma cells compared with flow cytometry. Med Sci Monit Basic Res. 2015 Feb 9;21:15-20. doi: 10.12659/MSMBR.893327, PMID 25664686, PMCID PMC4332266.
Mesbah M, Douadi T, Sahli F, Issaadi S, Boukazoula S, Chafaa S. Synthesis, characterization, spectroscopic studies and antimicrobial activity of three new Schiff bases derived from Heterocyclic moiety. J Mol Struct. 2018 Jan;1151:41-8. doi: 10.1016/j.molstruc.2017.08.098. MOLSTRUC.2017.08.098.
Vijayapriya M, Mahalakshmi S, Christiya CR. In vitro studies on antiproliferative effect of Euphorbia hirta L. Leaves. Annals of the Romanian Society of Cell Biology. 2021;25(4):1455-67.
Mukherjee S, Pal CK, Kotakonda M, Joshi M, Shit M, Ghosh P. Solvent induced distortion in a square planar copper(II) complex containing an azo-functionalized schiff base: synthesis, crystal structure, in vitro fungicidal and anti-proliferative, and catecholase activity. J Mol Struct. 2021 Dec;1245:131057. doi: 10.1016/j.molstruc.2021.131057.
RR, Shafreen M, Kumar N. Inhibition of proliferation in ovarian cancer cell line (PA-1) by the action of green compound ”betanin”. Appl Biochem Biotechnol. 2022 Jan;194(1):71-83. doi: 10.1007/s12010-021-03744-0. PMID 34762269.
Suresh P, Xavier AS, VPK KP. Anticancer activity of cissus quadrangularis l. methanolic extract against mg63 human osteosarcoma cells– an in vitro evaluation using cytotoxicity assay. Biomed Pharmacol J. 2019;12(2):975-80. doi: 10.13005/BPJ/1724.
Curcic MG, Stankovic MS, Mrkalic EM, Matovic ZD, Bankovic DD, Cvetkovic DM. Antiproliferative and proapoptotic activities of methanolic extracts from Ligustrum vulgare L. as individual treatment and in combination with palladium complex. Int J Mol Sci. 2012;13(2):2521-34. doi: 10.3390/ijms13022521. PMID 22408469, PMCID PMC3292038.
Barathan M, Zulpa AK, Vellasamy KM, Mariappan V, Shivashekaregowda NKH, Ibrahim ZA. Cytotoxic activity of isoniazid derivative in human breast cancer cells. In Vivo. 2021 Sep-Oct;35(5):2675-85. doi: 10.21873/invivo.12551, PMID 34410956, PMCID PMC8408694.
Park TS, Donnenberg VS, Donnenberg AD, Zambidis ET, Zimmerlin L. Dynamic interactions between cancer stem cells and their stromal partners. Curr Pathobiol Rep. 2014 Mar 1;2(1):41-52. doi: 10.1007/s40139-013-0036-5, PMID 24660130, PMCID PMC3956651.
Narendhiran S, Mohanasundaram S, Arun J, Saravanan L, Catherine L, Subathra M. Comparative study in larvicidal efficacy of medicinal plant extracts against culex quinquefasciatus. Int J Res Plant Sci. 2014;4(1):22-5.
Coleman RE. Metastatic bone disease: clinical features, pathophysiology and treatment strategies. Cancer Treat Rev. 2001 Jun;27(3):165-76. doi: 10.1053/ctrv.2000.0210, PMID 11417967.
Lewis VO. What’s new in musculoskeletal oncology. J Bone Joint Surg Am. 2009 Jun;91(6):1546-56. doi: 10.2106/JBJS.I.00375. PMID 19487537.
Picci P, Ferrari S, Bacci G, Gherlinzoni F. Treatment recommendations for osteosarcoma and adult soft tissue sarcomas. Drugs. 1994 Jan;47(1):82-92. doi: 10.2165/00003495-199447010-00006, PMID 7510623.
Bacci G, Longhi A, Versari M, Mercuri M, Briccoli A, Picci P. Prognostic factors for osteosarcoma of the extremity treated with neoadjuvant chemotherapy: 15-year experience in 789 patients treated at a single institution. Cancer. 2006 Mar 1;106(5):1154-61. doi: 10.1002/cncr.21724, PMID 16421923.
Doss VA, Mohanasundaram S, Maddisetty P. Analysis of hydroethanolic extract of Senna alata (L.) to screen bioactive compounds with inhibitory activity on lipid peroxidation, in vitro antibacterial and antidiabetic efficacy. Int J Pharm Sci. 2016;6(1):1360-6.
Stiller CA, Craft AW, Corazziari I, EUROCARE Working Group. Survival of children with bone sarcoma in Europe since 1978: results from the Eurocare study. Eur J Cancer. 2001 Apr;37(6):760-6. doi: 10.1016/s0959-8049(01)00004-1, PMID 11311651.
Liu K, Liu PC, Liu R, Wu X. Dual AO/EB staining to detect apoptosis in osteosarcoma cells compared with flow cytometry. Med Sci Monit Basic Res. 2015 Feb 9;21:15-20. doi: 10.12659/MSMBR.893327, PMID 25664686, PMCID PMC4332266.
Rangarajan N, Sampath, Dass Prakash M, Mohanasundaram S. UV Spectrophotometry and FTIR analysis of phenolic compounds with antioxidant potentials in glycyrrhiza glabra and zingiber officinale. IJRPS 2021;12(1):877-83. doi: 10.26452/ijrps.v12i1.4215.
Fletcher CDM, Unni KK. Pathology and genetics of tumours of soft tissue and bone. In: Mertens F, editor: World Health Organization Classification of tumours. Lyon, France: IARC Press; 2002.
Cserni G, Chmielik E, Cserni B, Tot T. The new TNM-based staging of breast cancer. Virchows Arch. 2018 May;472(5):697-703. doi: 10.1007/s00428-018-2301-9. PMID 29380126.
Arndt CA, Rose PS, Folpe AL, Laack NN. Common musculoskeletal tumors of childhood and adolescence. Mayo Clin Proc. 2012 May;87(5):475-87. doi: 10.1016/j.mayocp.2012.01.015. PMID 22560526, PMCID PMC3538469.
Rangarajan N, Sangeetha R, Mohanasundaram S, Sampath, Porkodi K, Dass Prakash MV. Additive inhibitory effect of the peels of Citrus limon and Citrus sinensis against amylase and glucosidase activity. IJRPS 2020;11(4):6876-80. doi: 10.26452/ijrps.v11i4.3661.
Al-Mosawi SK, Al-Shamkhani ZA. New heterocyclic schiff base and azetidinone as antibacterial agents. Asian J Res Chem. Sep 2014;7(9):801-4.
Kumar KS, Ganguly S, Veerasamy R, De Clercq E. Synthesis, antiviral activity and cytotoxicity evaluation of Schiff bases of some 2-phenyl quinazoline-4(3)H-ones. Eur J Med Chem. 2010 Nov;45(11):5474-9. doi: 10.1016/j.ejmech.2010.07.058. PMID 20724039, PMCID PMC7115544.
Sivakumar S, Mohanasundaram S, Rangarajan N, Sampath V, Velayutham Dass Prakash MV. In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of severe acute respiratory syndrome caused by novel coronavirus 2 with the FDA-approved nonprotein antiviral drugs. J Appl Pharm Sci. 2022;12(05):104-19. doi: 10.7324/JAPS.2022.120508.
Shanty AA, Philip JE, Sneha EJ, Prathapachandra Kurup MR, Balachandran S, Mohanan PV. Synthesis, characterization and biological studies of Schiff bases derived from heterocyclic moiety. Bioorg Chem. 2017 Feb;70:67-73. doi: 10.1016/j.bioorg.2016.11.009. PMID 27894775.
Pontiki E, Hadjipavlou Litina D, Chaviara AT. Evaluation of anti-inflammatory and antioxidant activities of copper (II) Schiff mono-base and copper(II) Schiff base coordination compounds of dien with heterocyclic aldehydes and 2-amino-5-methyl-thiazole. J Enzyme Inhib Med Chem. 2008 Dec;23(6):1011-7. doi: 10.1080/14756360701841251, PMID 19005947.
Amer S, El-Wakiel N, El-Ghamry H. Synthesis, spectral, antitumor and antimicrobial studies on Cu(II) complexes of purine and triazole Schiff base derivatives. J Mol Struct. 2013;1049:326-35. doi: 10.1016/j.molstruc.2013.06.059.
Mohanasundaram S, Doss VA, Haripriya G, Varsha M, Daniya S, Madhankumar. GC-MS analysis of bioactive compounds and comparative antibacterial potentials of aqueous, ethanolic and hydroethanolic extracts of Senna alata L. against enteric pathogens. Int J Res Pharm Sci. 2017;8(1):22-7.
Bensaber SM, Allafe HA, Ermeli NB, Mohamed SB, Zetrini AA, Alsabri SG. Chemical synthesis, molecular modelling, and evaluation of anticancer activity of some pyrazol-3-one Schiff base derivatives. Med Chem Res. 2014;23(12):5120-34. doi: 10.1007/s00044-014-1064-3.
Sinha A, Banerjee K, Banerjee A, Das S, Choudhuri SK. Synthesis, characterization and biological evaluation of a novel vanadium complex as a possible anticancer agent. J Organomet Chem. 2014;772-773:34-41. doi: 10.1016/j.jorganchem.2014.08.032.
Wang Q, Sivakumar K, Mohanasundaram S. Impacts of extrusion processing on food nutritional components. Int J Syst Assur Eng Manag. 2022;13(S1):364-74. doi: 10.1007/s13198-021-01422-2.
Qin W, Long S, Panunzio M, Biondi S. Schiff bases: a short survey on an evergreen chemistry tool. Molecules. 2013 Oct 8;18(10):12264-89. doi: 10.3390/molecules181012264, PMID 24108395, PMCID PMC6270622.
Mohanasundaram S, Rangarajan N, Sampath V, Porkodi K, Pennarasi M. GC-MS and HPLC analysis of antiglycogenolytic and glycogenic compounds in kaempferol 3-O–gentiobioside containing Senna alata L. leaves in experimental rats. Translational Metab Syndr Research. 2021;4:10-7.
Uddin E, Islam R, Bitu NA, Hossain S, Uddin N. Biological applications of isoniazid derived Schiff base complexes: an overview. Asian J Res Biochem. 2020;6(3):17-31. doi: 10.9734/AJRB/2020/v6i330118.
Rodrigues FA, Oliveira AC, Cavalcanti BC, Pessoa C, Pinheiro AC, de Souza MV. Biological evaluation of isoniazid derivatives as an anticancer class. Sci Pharm. 2014;82(1):21-8. doi: 10.3797/scipharm.1307-25, PMID 24634839, PMCID PMC3951230.
de Souza MV. Current status and future prospects for new therapies for pulmonary tuberculosis. Curr Opin Pulm Med. 2006 May;12(3):167-71. doi: 10.1097/01.mcp.0000219264.42686.c9. PMID 16582670.
Keri RS, Sasidhar BS, Nagaraja BM, Santos MA. Recent progress in the drug development of coumarin derivatives as potent antituberculosis agents. Eur J Med Chem. 2015 Jul 15;100:257-69. doi: 10.1016/j.ejmech.2015.06.017. PMID 26112067.
Black M, Hussain H. Hydrazine, cancer, the internet, isoniazid, and the liver. Ann Intern Med. 2000 Dec 5;133(11):911-3. doi: 10.7326/0003-4819-133-11-200012050-00016, PMID 11103062.
Doss VA, Maddisetty P, Mohanasundaram S. Biological active compounds with various medicinal values of strychnos nux-vomica–a pharmacological summary. J Glob Trends Pharm Sci. 2016;7(1):3044-7.
Berkesi O, Kortvelyesi T, Hetenyi C, Nemeth T, Palinko I. Hydrogen bonding interactions of benzylidene type Schiff bases studied by vibrational spectroscopic and computational methods. Phys Chem Chem Phys. 2003;5(10):2009-14. doi: 10.1039/B301107K.
Yong J, Mainsah E, Ntum S, Ndifon P. Synthesis, characterization and antibacterial studies of some isoniazid-derived schiff bases. IRJPAC 2016;12(1):1-8. doi: 10.9734/IRJPAC/2016/27100.
Rodrigues FA, Oliveira AC, Cavalcanti BC, Pessoa C, Pinheiro AC, de Souza MV. Biological evaluation of isoniazid derivatives as an anticancer class. Sci Pharm. 2014;82(1):21-8. doi: 10.3797/scipharm.1307-25, PMID 24634839, PMCID PMC3951230.
Cheng J, Wang X, Qiu L, Li Y, Marraiki N, Elgorban AM. Green synthesized zinc oxide nanoparticles regulates the apoptotic expression in bone cancer cells MG-63 cells. J Photochem Photobiol B. 2020 Jan;202:111644. doi: 10.1016/j.jphotobiol.2019.111644. PMID 31770706.
Karthikeyan V, Sivakumar K, Gokuldass A, Mohana Sundaram S. Studies on larvicidal activity of leucas aspera, vitex negundo and eucalyptus against culex quinquefasciatus collected from coovum river of Chennai, India. Asian J Pharm Sci Clin Res. 2012;5(3):1-4.
Gherghi ICh, Girousi ST, Voulgaropoulos AN, Tzimou Tsitouridou R. Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE). Talanta. 2003 Oct 17;61(2):103-12. doi: 10.1016/S0039-9140(03)00238-8, PMID 18969168.
Leite M, Quinta Costa M, Leite PS, Guimaraes JE. Critical evaluation of techniques to detect and measure cell death-study in a model of UV radiation of the leukaemic cell line HL60. Anal Cell Pathol. 1999;19(3-4):139-51. doi: 10.1155/1999/176515, PMID 10866276.
Mohanasundaram S, Rangarajan N, Sampath V, Porkodi K, Dass Prakash MV, Monicka N. GC-MS identification of anti-inflammatory and anticancer metabolites in edible milky white mushroom (Calocybe indica) against human breast cancer (MCF-7) cells. Res J Pharm Technol. 2021;14(8):4300-6.
Ramadevi P, Singh R, Prajapati A, Gupta S, Chakraborty D. Cu(II) complexes of isoniazid schiff bases: DNA/BSA binding and cytotoxicity studies on A549 cell line. Adv Chem. 2014. doi: 10.1155/2014/630575.
Bharathi P, Elavarasi N, Mohanasundaram S. Studies on rate of biodegradation of vegetable oil (Cocunut oil) by using Pseudomonas sp. Int J Environ Biol. 2012;2(2):12-9.
Baskic D, Popovic S, Ristic P, Arsenijevic NN. Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biol Int. 2006 Nov;30(11):924-32. doi: 10.1016/j.cellbi.2006.06.016. PMID 16895761.
Ujjan JA, Morani W, Memon N, Mohanasundaram S, Nuhmani S, Singh BK. Force platform-based intervention program for individuals suffering with neurodegenerative diseases like Parkinson. Comput Math Methods Med. 2022. doi: 10.1155/2022/1636263, PMID 35082910.
Hosseini A, Ghorbani A. Cancer therapy with phytochemicals: evidence from clinical studies. Avicenna J Phytomed. 2015 Mar-Apr;5(2):84-97. PMID 25949949, PMCID: PMC4418057.
Mohanasundaram S, Victor Arokia Doss VA, Prasad Maddisetty P, Magesh R, Sivakumar K, Subathra M. Pharmacological analysis of hydroethanolic extract of Senna alata (L.) for in vitro free radical scavenging and cytotoxic activities against Hep G2 cancer cell line. Pak J Pharm Sci. 2019;32(3):931-4.
Cui J, Jing W, Guo S, Cui J, Zhou M. Triphala suppresses ovarian cancer cell proliferation through induction of apoptosis and ROS in vitro. International Journal of Pharmacology. 2021;17(7):491-8. https://doi.org/10.3923/ijp.2021.491.498.
Vijayalakshmi A, Sindhu G. Umbelliferone arrest cell cycle at G0/G1 phase and induces apoptosis in human oral carcinoma (KB) cells possibly via oxidative DNA damage. Biomed Pharmacother. 2017 Aug;92:661-71. doi: 10.1016/j.biopha.2017.05.128. PMID: 28586740.
Published
How to Cite
Issue
Section
Copyright (c) 2022 MUNI HEMALATHA GILAKARA, KANDASAMY THIRUNAVUKKARASU, C. RAMESH KUMAR
This work is licensed under a Creative Commons Attribution 4.0 International License.
