ANTIOXIDANT AND ANTI-DYSLIPIDEMIC ACTIVITY OF BROWN SEAWEED (SARGASSUM POLYCYSTUM) EXTRACT IN RATS FEED WITH HIGH-FAT CONTENT

Authors

  • SARAH ZAIDAN Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia
  • SYAMSUDIN Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia, Postgraduate Program, Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia
  • DIAH KARTIKA PRATAMI Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia
  • HANA AMARANI SASTRA Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia
  • FIORINTINA SUTIKNO Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia
  • DESI NADYA AULENA Faculty of Pharmacy, Universitas Pancasila, South Jakarta, 12640, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.06

Keywords:

Brown seaweed (Sargassum polycystum), Antioxidant, SOD, High-fat feed, Dyslipidemia, Cholesterol

Abstract

Objective: This research aimed to obtain the antioxidant and antidyslipidemic activity of brown seaweed (S. polycystum) extract in vivo.

Methods: Two tests, such as antioxidant and anti-dyslipidemia, were conducted on the sample animals. Also, the number of Wistar rats was divided into 2 sections which consisted of 6 treatment groups, respectively. The test animals received high-fat feed for 35 d, after which there administered brown seaweed extract for 14 d. An antioxidant test consisting of 6 treatment groups, such as normal, negative, positive control (vitamin E), doses of 50, 100, and 200 mg/kg BW, was conducted in the first group. Meanwhile, the anti-dyslipidemia test consisting of 6 treatment groups, including normal, negative, positive (simvastatin), doses of 50, 100, and 200 mg/kg BW, was carried out in the second group. Hematological and statistical analysis was measured and performed using a 300 micro lab photometer as well as ANOVA, respectively.

Results: The antioxidant test results showed that superoxide dismutase (SOD) activity obtained a percentage increase of 63.41%, 75.01%, and 177.11%, while the dyslipidemia test results showed that after the administration of brown seaweed (S. polycystum) extract at a dose of 200 mg/kg BW, there was a significant difference between the negative controls, with p less than α (0.05), such as total cholesterol (0.000) and triglycerides (0.000). The percentage decrease in total cholesterol levels in the dose group was 31.98%, 41.47%, and 49.45%, with triglyceride levels of 30.65%, 37.78%, and 47.96%, respectively.

Conclusion: Considering these results, it was concluded that brown seaweed extract has an antioxidant activity on SOD parameters as well as an antidyslipidemic activity on total cholesterol and triglyceride parameters. Therefore, the most effective dose in improving the levels of total cholesterol and triglycerides is 200 mg/kg BW.

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References

Yoeantafara A, Martini S. Pengaruh pola makan terhadap Kadar Kolesteroltotal. J Mkmi. 2017;13(4):304-9. doi: 10.30597/mkmi.v13i4.2132.

Siburian M, Superoksida AA. Dismutase pada hati tikus Hiperkolesterolemia yang diberi ekstrak kulit mahoni (Swietenia macrophylla) [thesis]. IPB University; 2011.

Erwinanto SA, Putranto J, Tedjasukmana P, Sukmawan R, Suyawan R. Panduan Tata Laksana dislipidemia 2017. Jakarta Perhimpun Dr Spes Kardiovask Indones; 2017. p. 1-49.

Novidiyanto N, Farmawati A, Lestari LA. Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap Kadar malondealdehid (MDA) plasma dan jaringan hati tikus sprague dawley yang diberi pakan lemak tinggi. J Gizi Klin Indones. 2016;13(2):82-9. doi: 10.22146/ijcn.22923.

Akbarzadeh S, Gholampour H, Farzadinia P, Daneshi A, Ramavandi B, Moazzeni A. Anti-diabetic effects of Sargassum oligocystum on streptozotocin-induced diabetic rat. Iran J Basic Med Sci. 2018;21(3):342-6. doi: 10.22038/ IJBMS.2018.25654.6329, PMID 29511502.

Diachanty S, Nurjanah N, Abdullah A. Antioxidant activities of various brown seaweeds from Seribu Islands. J Pengolah Has Perikan Indones. 2017;20:305-18.

BPOM RI, Nomor PB. Tahun 2014 Tentang Pedoman Uji Toksisitas Non Klinik secara in vivo. Badan Pengawas Obat Makanan. 2014;7:1-165.

Chen L, Long R, Huang G, Huang H. Extraction and antioxidant activities in vivo of pumpkin polysaccharide. Ind Crops Prod. 2020;146:112199. doi: 10.1016/j.indcrop.2020.112199.

Belayneh YM, Birhanu Z, Birru EM, Getenet G. Evaluation of in vivo antidiabetic, antidyslipidemic, and in vitro antioxidant activities of hydromethanolic root extract of Datura stramonium L. (Solanaceae). J Exp Pharmacol. 2019;11:29-38. doi: 10.2147/JEP.S192264, PMID 31114400.

BPOM RI. Pedoman Uji Toksisitas Nonklinik Secara in vivo. [Badan Pengawas Obat dan Makanan Republik Indones Jakarta]. 2014;13:1-18.

Yasmeen H, Hasnain S. In vitro antioxidant effect of camellia sinensis on human cell cultures. Pak J Pharm Sci. 2015;28(5):1573-81. PMID 26408866.

Yang RL, Shi YH, Hao G, Li W, Le GW. Increasing oxidative stress with progressive hyperlipidemia in human: relation between malondialdehyde and atherogenic index. J Clin Biochem Nutr. 2008;43(3):154-8. doi: 10.3164/jcbn.2008044, PMID 19015749.

Widiyantoro A, Wardoyo EPW, Sayekti E. Aktivitas ektrak buah Makasar (Brucea javanica L. Merr.) terhadap radikal anion superoksida secara in vivo. J Penelit Saintek. 2010;15:1-8.

Astuti S. Isoflavon kedelai dan potensinya sebagai penangkap radikal bebas. J Teknol Ind Has Pertan. 2012;13:126-36.

Beppu F, Hosokawa M, Niwano Y, Miyashita K. Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-A(y) mice. Lipids Health Dis. 2012;11:112. doi: 10.1186/1476-511X-11-112. PMID 22962999.

Gammone MA, D’Orazio N. Anti-obesity activity of the marine carotenoid fucoxanthin. Mar Drugs. 2015;13(4):2196-214. doi: 10.3390/md13042196, PMID 25871295.

Published

28-06-2022

How to Cite

ZAIDAN, S., SYAMSUDIN, PRATAMI, D. K., SASTRA, H. A., SUTIKNO, F., & AULENA, D. N. (2022). ANTIOXIDANT AND ANTI-DYSLIPIDEMIC ACTIVITY OF BROWN SEAWEED (SARGASSUM POLYCYSTUM) EXTRACT IN RATS FEED WITH HIGH-FAT CONTENT. International Journal of Applied Pharmaceutics, 14(3), 27–30. https://doi.org/10.22159/ijap.2022.v14s3.06

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