• MUHAMMAD ABDURRAHMAN MUNIR Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia https://orcid.org/0000-0001-6129-6202
  • AHLAM INAYATULLAH Department of Food Technology, Faculty of Chemical Engineering, Politeknik Sriwijaya, Palembang, 30139, Indonesia
  • SOFIAN IBRAHIM Malaysian Nuclear Agency, 43000 Kajang, Bangi, Malaysia
  • IMRAM RADNE RIMBA PUTRI Department of Hospital Administration, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia
  • EMELDA EMELDA Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia
  • ANNISA FATMAWATI Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia
  • NURHIDAYANTI NURHIDAYANTI Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia




Acetaminophen, Pharmacies, Spectrophotometer, Syrups, Tablets


Objective: This study aimed to verify the paracetamol level in some fabricated tablets and syrups in Indonesian pharmacies.

Methods: The fabricated tablets and syrups were analyzed using a spectrophotometer UV that was assisted by the chemometric approach. Partial least squares (PLS) and principal component regression (PCR) were the chemometric methods employed to verify the paracetamol level in pharmaceutical products. There were 25 different samples (tablets and syrups) applied in this study. The validation study was employed in this study to verify the approach according to the ICH guidelines. The double-distilled water was applied as a solvent before the samples were analyzed using a spectrophotometer.

Results: This technique was efficient and require double-distilled water only as a solvent. The results of this study reveal that there was a deviation in absorbance of the samples with RSD ranging from (0.15-0.45). The technique was linear, ranging from 1.0–6.0 µg·ml-1, with an R2 (0.9991) obtained at 242 nm. The percentage recovery was applied to study the accuracy of the technique and was acquired at 99.18%. The results have shown that the approach was the potential to be applied in estimating the level of paracetamol in tablets and syrups.

Conclusion: The detection of paracetamol levels in tablets and syrups using UV spectrophotometric showed satisfactory outcomes. The application of the chemometric approach by using PLC and PCR as the statistical assessment indicated that there was no significant distinction among the validated methods. Furthermore, the method can be used by industries particularly small industries to secure medicines that comply with Indonesian rules.


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