INHIBITORY EFFECT OF CURCUMIN INCORPORATED IN CHITOSAN NANOPARTICLE ON α-AMYLASE AND α-GLUCOSIDASE ACTIVITIES
DOI:
https://doi.org/10.22159/ijap.2022.v14s4.OP06Keywords:
Curcumin, Nanoparticle, α-amylase, α-glucosidaseAbstract
Objective: This research was conducted to determine inhibition activity of curcumin nanoparticles against α-amylase and α-glucosidase enzymes.
Methods: Curcumin nanoparticle was made by ionic gelation method using chitosan as cation, sodium tripolyphosphate as polyanion, and tween 80 as surfactant. Curcumin nanoparticles were tested for inhibitory activity of α-amylase and α-glucosidase enzymes using UV-Vis spectrophotometry at λ= 595 nm and 305 nm, respectively.
Results: Curcumin nanoparticles produced have 198.1 nm of particle size with PdI value of 0.349 and zeta potential value of-8,33 mV. The IC50 value of curcumin nanoparticles against α-amylase was 56.140 ppm, while acarbose was 63.32 ppm. While the IC50 value against α-glucosidase was 3.95 ppm and 4.11 ppm for curcumin nanoparticles and acarbose, respectively.
Conclusion: It can be concluded that curcumin nanoparticles have great potential as antihyperglycemic by inhibiting α-amylase and α-glucosidase enzymes.
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Copyright (c) 2022 WINDA T WULANDARI, LILIS TUSLINAH, ADE Y APRILLIA, RATNA ANGGRAENI, SHAFA A BUDIANI
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