ISOLATION, IDENTIFICATION, AND QUANTIFICATION OF MAJOR FLAVONOID IN LEAVES OF PERESKIA BLEO (KUNTH) DC

NYI MEKAR SAPTARINI; RESMI MUSTARICHIE; IRMA ERIKA HERAWATI; GINAYANTI HADISOEBROTO

doi:10.22159/ijap.2022.v14s4.PP19

Authors

NYI MEKAR SAPTARINI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, West Java, Indonesia, 45363
RESMI MUSTARICHIE Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, West Java, Indonesia, 45363
IRMA ERIKA HERAWATI Indonesian School of Pharmacy, West Java, Indonesia, 40266
GINAYANTI HADISOEBROTO Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Al Ghifari University, West Java, Indonesia, 40293

DOI:

https://doi.org/10.22159/ijap.2022.v14s4.PP19

Keywords:

Catechin, Chromatographic separations, Flavonol, Shifting reagent, Purity

Abstract

Objective: This study aimed to isolate, identify, and quantify major flavonoid in P. bleo leaves from West Java Regency, Indonesia.

Methods: Isolation began with maceration, followed by liquid-liquid extraction and various chromatographic separations. Identification of isolates was carried out using 2D TLC and shifting reagents. Quantification was determined with the colorimetric method.

Results: The results showed that extract, ethyl acetate, and distilled water fractions contain flavonoids. The flavonoid screening showed positive results for flavonols. The vacuum liquid chromatography separated 21 sub-fractions; the 12^th and 13^th sub-fractions were predicted to contain flavonols.

Conclusion: The novelty of this study was the isolation and identification of the major flavonoid of P. bleo leaves originating from West Java Regency, Indonesia, i.e. catechin. The content of the compound was 3.795±0.096 g QE/ml with a purity of 94.89%.

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