THE FORMATION AND CHARACTERIZATION OF COLLAGEN LIQUID CRYSTALS FROM SNAKEHEAD FISH SKIN (CHANNA STRIATA)

RINI AGUSTIN; AQSHA MUTIA QALBI; RAHMI NOFITA R.

doi:10.22159/ijap.2023.v15s1.05

Authors

RINI AGUSTIN Faculty of Pharmacy, Andalas University, Padang, West Sumatra, Indonesia
AQSHA MUTIA QALBI Faculty of Pharmacy, Andalas University, Padang, West Sumatra, Indonesia
RAHMI NOFITA R. Faculty of Pharmacy, Andalas University, Padang, West Sumatra, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023.v15s1.05

Keywords:

Please include 3-10 keywords for indexing purposes

Abstract

Objective: Liquid crystals are special state of matters which have regularity of solid arrangement but had a liquid-like flow characteristics. Collagen is a biopolymer that qualified for requirements as the system of a liquid crystal because it is mesogenic and rigid in a triple-helix section. There are various sources of collagen that have been used; one of them is snakehead fish skin (Channa striata).

Methods: The stages of research were collagen isolation, collagen identification, liquid crystals formation, and characterization. Collagen liquid crystals were formed by lyotropic method using 0.5 M acetic acid and treated with and without sonication at 30, 60, and 80 mg/ml concentrations. The formation of Liquid crystal phase characterized by using Polarization Ligh microscopy.

Results: Mesophase analysis using polarized light microscope showed the presence of cholesteric phase (fingerprint pattern) which seen from the lowest concentration used in this study (30 mg/ml). The increasing of collagen concentration and sonication treatment can trigger the formation of clearly liquid crystal cholesteric phase under polarized light microscope. Infrared spectra of collagen liquid crystals both sonicated or not, showed no change in triple-helix.

Conclusion: The formation of lyotropic liquid crystal of collagen from snakehead fish skin showed the cholesteric pattern without changing the triple-helix collagen structure.

Downloads

Download data is not yet available.

References

Yogeshvar T. Liquid crystals: an approach to a different state of matter. The Pharma. Innov J. 2018;7(6):540-5.

Silvestrini AVP, Caron AL, Viegas J, Praça FG, Bentley MVLB. Advances in lyotropic liquid crystal systems for skin drug delivery. Expert Opin Drug Deliv. 2020;17(12):1781-805. doi: 10.1080/17425247.2020.1819979, PMID 32886531.

Kawai M, Ibaraki H, Takashima Y, Kanazawa T, Okada H. Development of a liquid crystal formulation that can penetrate the stratum corneum for intradermal delivery of small interfering RNA. Mol Pharm. 2021;18(3):1038-47. doi: 10.1021/acs.molpharmaceut.0c00997, PMID 33395310.

Wesam RK, Hiroarki T, Kenji S. Percutanous penetration Enchancers chemical methods in penetration Enchancement. New York: Springer Berlin Heidelberg; 2015.

Portier F, Teulon C, Nowacka Perrin A, Guenneau F, Schanne Klein MC, Mosser G. Stabilization of collagen fibrils by gelatin addition: a study of collagen/gelatin dense phases. Langmuir. 2017;33(45):12916-25. doi: 10.1021/acs.langmuir.7b02142, PMID 29087724.

Baron M, Stepto RFT. Definitions of basic terms relating to polymer liquid crystals (IUPAC Recommendations 2001). Pure Appl Chem. 2002;74(3):493-509. doi: 10.1351/ pac200274030493.

Giraud Guille MM, Mosser G, Belamie E. Liquid crystallinity in collagen systems in vitro and in vivo. Curr Opin Colloid Interface Sci. 2008;13(4):303-13. doi: 10.1016/j.cocis.2008.03.002.

Besseau L, Giraud Guille MM. Stabilization of fluid cholesteric phases of collagen to ordered gelated matrices. J Mol Biol. 1995;251(2):197-202. doi: 10.1006/jmbi.1995.0426, PMID 7643395.

Min Z. Observation of liquid crystalline collagen with atomic force microscopy (AFM). J Bioresour Bioprod. 2016;1(3):139-44.

Jiang GA, Saba H, Yong Y, Min X, Yongsong L. Characterization of liquid crystals: a literature review. Rev Adv Mater Sci. 2016;44(4):398-406.

Giraud Guille M, Belamie E, Mosser G, Helary C, Gobeaux F, Vigier S. Liquid crystalline properties of type I collagen: perspectives in tissue morphogenesis. C R Chim. 2008;11(3):245-52. doi: 10.1016/j.crci.2007.05.005.

Rahmi N. Pembuatan film balutan primer yang mengandung kolagen kulit ikan gabus (Channa striata) dan pengaruh film terhadap penyembuhan luka bakar [disertasi]. Padang: Fakultas Farmasi Universitas Andalas; 2017.

Cao J, Duan Q, Liu X, Shen X, Li C. Extraction and physicochemical characterization of pepsin soluble collagens from golden pompano (Trachinotus blochii) skin and bone. J Aquat Food Prod Technol. 2019;28(8):837-47. doi: 10.1080/10498850.2019.1652216.

Badan Standardisasi Nasional. Kolagen kasar dari sisik ikan-syarat mutu dan pengolahan: SNI 8076-2014. Jakarta: badan standardisasi nasional; 2014.

Lebon M, Reiche I, Gallet X, Bellot Gurlet L, Zazzo A. Rapid quantification of bone collagen content by ATR-FTIR spectroscopy. Radiocarbon. 2016;58(1):131-45. doi: 10.1017/RDC.2015.11.

Liu HY, Li D, Guo SD. Studies on collagen from the skin of channel catfish (Ictalurus punctaus). Food Chem. 2007;101(2):621-5. doi: 10.1016/j.foodchem.2006.01.059.

Bak SY, Lee SW, Choi CH, Kim HW. Assessment of the influence of acetic acid residue on type I collagen during isolation and characterization. Materials (Basel). 2018;11(12):2518. doi: 10.3390/ma11122518, PMID 30545004.

Andi RR, Andi RA, Nur AB, Muhammad DM. Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata). J Food Technol Nutritions Culinary. 2020;3(2):87-93.

Ramasamy S, Ramadhar K. A novel enzymatic method for preparation and characterization of collagen film from swim bladder of fish rohu (Labeo rohita). Food Nutr Sci. 2015;6(15):1468-78.

Hegmann T, Qi H, Marx VM. Nanoparticles in liquid crystals: synthesis, self-assembly, defect formation and potential applications. J Inorg Organomet Polym. 2007;17(3):483-508. doi: 10.1007/s10904-007-9140-5.

Noitup P, Garnjanagoonchorn W, Morrissey MT. Fish skin type I collagen: characteristic comparison of albacore tuna (Thunnus alalunga) and silver-line grunt (Pomadasys kaakan). J Aquat Food Prod Technol. 2005;14(1):17-28. doi: 10.1300/J030v14n01_03.

Giraud Guille MM, Besseau L, Chopin C, Durand P, Herbage D. Structural aspects of fish skin collagen which forms ordered arrays via liquid crystalline states. Biomaterials. 2000;21(9):899-906. doi: 10.1016/s0142-9612(99)00244-6, PMID 10735466.

Minjian T, Shan D, Xiang M, Yanpeng J, Lihua L, Hong L. Collagen films with stabilized liquid crystalline phases and concerns on osteoblast behaviors. Mater Sci Eng. 2016;58(1):977-85.

Alexander T. Type-I collagen modeled as liquid crystal. Vol. 3. Rose: Hulman Undergraduate Research Publications; 2013. p. 1-10.

Price JC, Roach P, El Haj AJ. Liquid crystalline ordered collagen substrates for applications in tissue engineering. ACS Biomater Sci Eng. 2016;2(4):625-33. doi: 10.1021/acsbiomaterials.6b00030, PMID 33465864.

Vidal Bde C, Mello ML. Collagen type I amide I band infrared spectroscopy. Micron. 2011;42(3):283-9. doi: 10.1016/j.micron.2010.09.010, PMID 21134761.

John GW. The measurement instrumentation and sensors handbook. CRC Press LLC; 1999.