QUANTITATIVE ANALYSIS OF DEXTROMETHORPHAN-HBr, GUAIFENESIN AND DIPHENHYDRAMINE-HCl IN TABLET DOSAGE FORM BY SUCCESSIVE RATIO DERIVATIVE SPECTRA METHOD

RIDA EVALINA TARIGAN; MUHAMMAD ANDRY; RATIH KUMALA DEWI; MUHAMMAD AMIN NASUTION; MUHAMMAD FAUZAN LUBIS

doi:10.22159/ijap.2024v16i1.49222

Authors

RIDA EVALINA TARIGAN Departement of Pharmaceutical Chemistry, Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan, Sumatera Utara-20124, Indonesia https://orcid.org/0000-0002-1151-8038
MUHAMMAD ANDRY Departement of Pharmaceutical Chemistry, Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan, Sumatera Utara-20124, Indonesia https://orcid.org/0000-0001-5270-1288
RATIH KUMALA DEWI Departement of Pharmaceutical Chemistry, Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan, Sumatera Utara-20124, Indonesia
MUHAMMAD AMIN NASUTION Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah, Medan, Sumatera Utara-20147, Indonesia
MUHAMMAD FAUZAN LUBIS Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0001-9651-904X

DOI:

https://doi.org/10.22159/ijap.2024v16i1.49222

Keywords:

Dextromethorphan HBr, Guaifenesin, Diphenhydramine HCl, Spectrophotometry, Succesive ratio derivative

Abstract

Objective: This study aims to develop a spectrophotometric method with the successive ratio derivative spectra method using ethanol pro analysis solvent to obtain the results of the levels of Dextromethorphan (DEX) Hydrobromide (HBr), Guaifenesin (GUA) and, Diphenhydramine (DIF) Hydrochloric Acid (HCl) in tablet dosage form.

Methods: This method is straightforward to determine the levels of DEX HBr, GUA, and DIF HCl in the wavelength range of 200-400 nm using absorption, which is calculated experimentally using spectrophotometry UV-Vis.

Results: The maximum wavelengths of DEX HBr, GUA and DIF HCl were obtained at 278 nm, 273 nm, and 252 nm, respectively. The average % accuracy was obtained at 99.50% for DEX HBr, 99.91% for GUA, and 99.98% for DIF HCl in dosage forms.

Conclusion: This method was successfully applied to determine the levels of DEX HBr, GUA and DIF HCl in tablet preparations and met the validation requirements.

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