NANOENCAPSULATION OF ETHANOL EXTRACT OF PAPAYA LEAF (CARICA PAPAYA LINN.) USING CHITOSAN AND TESTING ITS EFFECTIVENESS AS AN ANTI-INFLAMMATORY

MOHAMAD ANDRIE; WINTARI TAURINA

doi:10.22159/ijap.2024v16i2.49992

Authors

MOHAMAD ANDRIE Departement of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak, West Kalimantan, Indonesia https://orcid.org/0009-0007-7167-7878
WINTARI TAURINA Departement of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak, West Kalimantan, Indonesia https://orcid.org/0009-0008-8930-4188

DOI:

https://doi.org/10.22159/ijap.2024v16i2.49992

Keywords:

Anti-inflammation, Ethanol extract of papaya leaves, Chitosan-tripolyphosphate, Nanoencapsulation

Abstract

Objective: Papaya is a plant typical of West Kalimantan which has many properties such as anti-inflammatory, analgesic, antimalarial, and antibacterial. This research aims to formulate ethanol extract of papaya leaves into a nanoencapsulated preparation and test its effectiveness as an anti-inflammatory.

Methods: Nanoparticle formulations made with the ionic gelation method use polymer chitosan (0.25%-1%) with crosslinker sodium tripolyphosphate (0.25%). Nanoencapsulation ethanol extract of papaya leaf was evaluated for characteristics including particle size distribution, index polydispersity, zeta potential, particle morphology, and entrapment efficiency. Furthermore, The efficacy of anti-inflammatory nanoencapsulation was then evaluated on male Wistar rats with carrageenan-induced inflammation using doses of 100 mg/kg and 200 mg/kg. The assessment of anti-inflammatory activity utilized the Rat hind paw edema method by observing the development of inflammation in the volume of the soles of the test animals' paws.

Results: The results of nanoencapsulation characterization showed that papaya ethanol extract in Formula 1 with a ratio of Chitosan: Papaya Leaf Extract Ethanol: NaTPP = 6:1:1 was the best formula, exhibiting an average particle size of 217.3±47.8 nm, a polydispersity index value of 0.271, a zeta potential value of+34.3 mV, an entrapment efficiency value of 65.54, and a particle morphology that is less spherical. The test for anti-inflammatory activity of papaya leaf ethanol extract nanoparticles, administered orally at a dosage of 200 mg/kgBW, demonstrated the highest percentage of anti-inflammatory efficacy at 61.538%. In comparison, the positive control group (diclofenac sodium) exhibited 54.325%, and the low-dose group (100 mg/kgBW) showed 51.585%. The results showed that the ethanol extract of papaya, when nanoencapsulated in chitosan nanoparticles, exhibits good characteristics and has significant potential for inhibiting inflammation in male Wistar rats induced by carrageenan.

Conclusion: The characterization results of the optimal chitosan-ethanol papaya leaf extract nanoparticles were obtained using Formula 1. Nanoparticles of chitosan-ethanol extract from papaya leaves at doses I and II exhibited anti-inflammatory activity that was not significantly different.

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References

Kee JL, Hayes ER. Pharmacology: a nursing process approach. Jakarta: EGC Publisher; 1993.

Ezekwe AS, Ify E, Osuocha UK. Hypoglycemic, hypolipidemic and body weight effects of the unripe pulp of Carica papaya L. using diabetic Albino rat model. J Pharmacogn Phytochem. 2014;2(6):109-14.

Sukadana IM, Santi RS, Juliarti NK. Antibacterial activity of triterpenoid compounds from papaya seeds (Carica papaya L.). Jurusan Kimia FMIPA Univ Udayana. 2008;2(1):15-8.

Sagnia B, Fedeli D, Casetti R, Montesano C, Falcioni G, Colizzi V. Antioxidant and anti-inflammatory activities of extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva medicinal plants collected in cameroon. PLOS ONE. 2014;9(8):e103999. doi: 10.1371/journal.pone.0103999, PMID 25090613.

Rehena JF. Test the activity of papaya leaf extract (Carica papaya. Linn) as an in vitro antimalarial. Faculty of Teacher Training and Education, Pattimura University; 2010;11(1):96-100.

Alex A. Adedapo and Vivian Eguonor, Orherhe. Antinociceptive and anti-inflammatory studies of the aqueous leaf extract of in laboratory animals. Nigeria: Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan. 2013;4(1):89-96.

Octavianus S, Fatimawali, Widya AL. Test the analgesic effect of ethanol extract of papaya leaves (Carica papaya L.) on male white mice (Mus mucculus). Manado: FMIPA UNSRAT Pharmacy Study Program. 2014;3(2):87-92.

Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets. 2009;8(3):229-35. doi: 10.2174/187152809788681029, PMID 19601883.

Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004;96(3):229-45. doi: 10.1254/jphs.crj04003x, PMID 15539763.

Rahmat D, Widowati W, Faried A, Nainggolan IM, Priyandoko D, Budiati A. Characterization of nanoparticles conditioned medium adipose tissue mesenchymal stem cell (CM-ATMSC). Int J App Pharm. 2022;14(3):104-6. doi: 10.22159/ijap.2022.v14s3.22.

Kartasasmita RH, Herowati R, Harmastuti N, Gusnidar T. Quercetin derivatives docking based on study of flavonoids interaction to cyclooxygenase-2. Bandung: Indo J Chem. 2009;9(2):297-302.

Rakesh B, Vijay M, Avinash D. New approaches in nanoparticulate drug delivery system-a review. Int J Curr Pharm Res. 2012;4(2):29-38.

Jain PK, Lee KS, El-Sayed IH, El-Sayed MA. Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. J Phys Chem B. 2006;110(14):7238-48. doi: 10.1021/jp057170o, PMID 16599493.

Muhaimin M, Chaerunisaa AY, Rostinawati T, Amalia E, Hazrina A, Nurhasanah S. A review on nanoparticles of Moringa oleifera extract: preparation, characterization, and activity. Int J App Pharm. 2023;15(4):43-51. doi: 10.22159/ijap.2023v15i4.47709.

Takeuchi H, Yamamoto H, Kawashima Y. Mucoadhesive nanoparticulate systems for peptide drug delivery. Adv Drug Deliv Rev. 2001;47(1):39-54. doi: 10.1016/s0169-409x(00)00120-4, PMID 11251244.

Andersen M, Markham KR. Flavonoids: chemistry, biochemistry, and applications. Taylor & Francis Group 2; 2006. p. 3-4.

Yulanda H. Extraction, fractionation, and characterization of Ginger rhizome starch. Bogor: Faculty of Mathematics and Natural Sciences, Bogor Agricultural Institute; 2007.

Mishra M, Kumar H, Singh RK, Tripathi K. Diabetes and nanomaterials. Digest Journey of Nanomaterials and Biostructures. 2008;3:109-13.

Setyawaty R, Aptuning BR, Dewanto D. Preliminary studies on the content of phytochemical compounds on the skin of salak fruit (Salacca zalacca). Pharm J Indones. 2020;6(1):1-6. doi: 10.21776/ub.pji.2020.006.01.1.

Anam S, Yusran M, Trisakti A, Ibrahim N, Khumaidi A, Ramadanil ZMS. Standardization of ethyl acetate extract of sanrego wood (Lunasia amara Blanco). Online Journal of Natural science. 2013;2(3):1-8.

Mardliyati E, Muttaqien SE, Setyawati DR. Synthesis of chitosan-tripolyphosphate nanoparticles using ionic gelation method: influence of concentration and volume ratio on particle characteristics; 2012.

Abdullah M, Khairurrijal. Review: nanomaterial characterization. J Nanosci Nanotechnol. 2009;1(2):1-9.

Wu Y, Yang W, Wang C, Hu J, Fu S. Chitosan nanoparticles as a novel delivery system for ammonium glycyrrhizinate. Int J Pharm. 2005;295(1-2):235-45. doi: 10.1016/j.ijpharm.2005.01.042, PMID 15848008.

Ab Cong HH, Khaziakhmetova VN, Zigashina LE. Rat paw oedema modeling and NSAIDs: timing of effects. Int J Risk Saf Med. 2015;27(s1):S76-7.

Arts JW, Kramer K, Arndt SS, Ohl F. The impact of transportation on physiological and behavioral parameters in wistar rats: implications for acclimatization periods. ILAR J. 2012;53(1):E82-98. doi: 10.1093/ilar.53.1.82, PMID 23382273.

Indonesian Food and Drug Authority. BPOM Regulation Number 18 of 2021 concerning Guidelines for Preclinical Pharmacodynamic Testing of Traditional Medicines. Jakarta: Indonesian Food and Drug Authority; 2021.

Mansjoer S. Anti-inflammatory effect of white ginger essential oil (Curcuma zedoaria Rosc.) on artificial edema in male white Wistar rats. Indonesian Pharm Mag. 1997;8:35-41.

A’yunin Q. Anti-inflammatory effect of tapak liman (Elephantopus scaber L.) leaf infusion on male white rats [thesis]. Surakarta: Faculty of Pharmacy, Muhammadiyah University of Surakarta; 2004.

Waree T. Chitosan nanoparticles: a promising system for drug delivery. Naresuan University Journal. 2003;11(3):51-66.

Voigt R. Pharmaceutical technology textbook. 5th ed. Yogyakarta: UGM-press; 1995.

Gupta RB, Kompella UB. Nanoparticle drug delivery for technology. Vol. 159. New York: Taylor & Francis Group; 2006.

Moura MJ, Faneca H, Lima MP, Gil MH, Figueiredo MM. In situ forming chitosan hydrogels prepared via ionic/covalent co-cross-linking. Biomacromolecules. 2011;12(9):3275-84. doi: 10.1021/bm200731x, PMID 21774479.

Mohanraj VJ, Nanopartikel CY. A review Nigeria. Trop J Pharm Res. 2006;5(1):561-73.

Lin Y, Sonaje K, Lin KM, Juang J, Mi F, Yang H. Multi-ion-crosslinked nanoparticles with pH-responsive characteristics for oral delivery of protein drugs. J Control Rel. 2008;132(2):141-9. doi: 10.1016/j.jconrel.2008.08.020.