PREPARATION, CHARACTERIZATION, AND IN VITRO EVALUATION OF GEL CONTAINING NANOPHYTO-PHOSPHOLIPID COMPLEX OF KOPASANDA LEAF EXTRACT (CHROMOLAENAODORATA (L.) R. M. KING & H. ROB)

SUCILAWATY RIDWAN; NURUL WAHYUNI; GUNAWAN PASARIBU; SAFWAN SAFWAN; WAHIDA HAJRIN; MARIAMA  FITRIANA; KAMELIA ROIYAN

doi:10.22159/ijap.2024v16i6.50147

Authors

SUCILAWATY RIDWAN Departement of Pharmacy, Faculty of Medicine and Health Sciences, Mataram University, Mataram-83125, Indonesia https://orcid.org/0009-0001-9182-8457
NURUL WAHYUNI National Research and Innovation Agency, Indonesia
GUNAWAN PASARIBU National Research and Innovation Agency, Indonesia
SAFWAN SAFWAN Department of Pharmacy, University of Muhammadiyah Mataram, Mataram, Indonesia https://orcid.org/0000-0002-8048-0301
WAHIDA HAJRIN Departement of Pharmacy, Faculty of Medicine and Health Sciences, Mataram University, Mataram-83125, Indonesia
MARIAMA FITRIANA Departement of Pharmacy, Faculty of Medicine and Health Sciences, Mataram University, Mataram-83125, Indonesia
KAMELIA ROIYAN Departement of Pharmacy, Faculty of Medicine and Health Sciences, Mataram University, Mataram-83125, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i6.50147

Keywords:

Nanophyto-phospholipid complex, Chromolaena odorata (L.) R.M. King & H. Rob), Antisolvent precipitation method, Gel, Permeation studies

Abstract

Objective: This study aims to prepare, characterize, and in vitro evaluation of the gel containing nanophyto-phospholipid complex of kopasanda leaf extract.

Methods: Kopasanda dried leaf was extracted by extraction reflux method, followed by total phenolic content of extract measurement using Spectrophotometry UV-Vis method.Thenanophyto-phospholipid complex was prepared using an antisolvent evaporation method with various ratios between extract and phospholipid of 1:1; 1:2; 1:3. The optimum ratio was evaluated by entrapment efficiency (%).The nanophyto-phospholipid complex formation was characterized by polydispersity index, particle size, Fourier Transform Infra-Red (FT-IR), and the Transmission Electron Microscope (TEM) method. The optimum nanophyto-phospholipid complex was formulated into gel preparation. The in vitro permeation study was performed to discover the influence of gel containing nanophyto-phospholipid complex compared with gel-containing extract without the nanophyto-phospholipid complex formation.

Results: Thetotal phenolic content of kopasanda leaf extract was 117.214±3.054 mg/GAE. The optimum ratio of kopasanda leaf extract and phospholipid was 1: 2 with entrapment efficiency (%), particle size, and polydispersity index equal to 99.897±0.001%, 130.1 nm and 0.394. The morphology of the nanophyto-phospholipid complex was spherical and the complex formation was confirmed by the FTIR spectrum. The permeation test showed that the gel containing nanophyto-phospholipid complex had better diffusion than the gel without the nanophyto-phospholipid complex formation.

Conclusion: The gel containing nanophyto-phospholipid complex formation exhibited the potential drug delivery system to increase the phenolic content permeation of kopasanda leaf extract.

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