DUMB CANE (DIEFFENBACHIA SEGUINE (JACQ.) SCHOTT) EXTRACT NANOEMULSION: PREPARATION, CHARACTERIZATION AND ITS ACTIVITY AS AN INFLAMMATION-INDUCING AGENT IN RATS

SITI SADIAH; NIDA NAZEERA JAPRI; LEONG KE YONG; DENNY WIDAYA lUKMAN; NI LUH PUTU IKA MAYASARI; LELIANA NUGRAHANING WIDI; DEWA AYU REGINA AMEL; HAMZAH ALFARISI

doi:10.22159/ijap.2024.v16s3.03

Authors

SITI SADIAH School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor-16680, West Java, Indonesia https://orcid.org/0000-0001-7513-4431
NIDA NAZEERA JAPRI School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor-16680, West Java, Indonesia
LEONG KE YONG School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor-16680, West Java, Indonesia
DENNY WIDAYA lUKMAN School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor-16680, West Java, Indonesia
NI LUH PUTU IKA MAYASARI School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor-16680, West Java, Indonesia
LELIANA NUGRAHANING WIDI Tropical Biopharmaca Research Center, IPB University, Bogor-16680, West Java, Indonesia
DEWA AYU REGINA AMEL Tropical Biopharmaca Research Center, IPB University, Bogor-16680, West Java, Indonesia
HAMZAH ALFARISI School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor-16680, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s3.03

Keywords:

Dieffenbachia seguine, Dumb cane, Sri rejeki, Nanoemulsion, Inflammation inducer agent

Abstract

Objective: This study aimed to characterize Dumb cane extract nanoemulsion for injection preparation and test its activity as an inflammation-inducing agent in rat intraplantar with IL-6 and TNF-alpha parameters in blood serum as well as the swelling response in paw.

Methods: Nanoemulsion was made from an emulsion base of olive oil, coconut oil, tween 80 propylene glycol, and water for injection, as well as varying concentrations of Dumb cane extract (1%, 2%, and 4%) using vortex mixing and sonication methods. The characterization included particle size, zeta potential, and polydispersity index using the Zetasizer tool. Induction was carried out intraplantar in male rats. The parameters observed were the volume of swelling in paw and IL-6 and TNF-alpha in blood serum.

Results: Dumb cane extract nanoemulsion concentrations of 1%, 2%, and 4% have particle size characteristics in the range of 20.3±0.17–30.1±0.68 nm, and zeta potential -31.4±1.59–33.1±1.33 mV. Dumb cane nanoemulsion can induce intraplantar inflammation with high IL-6 and TNF-alpha levels, significantly different from normal controls. Volume swelling occurred 4 h after intraplantar induction at a nanoemulsion concentration of 4%.

Conclusion: Dumb cane extract nanoemulsion can be an alternative agent for inducing intraplantar inflammation in rat models.

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