FORMULATION OF NANOSTRUCTURED LIPID CARRIER GEL FROM CALLUS EXTRACT OF MULBERRY LEAF (MORUS ALBA L.) WITH 2, 4-DICHLORO PHENOXY ACETIC ACID AND BENZYL AMINO PURINE AS CALLUS GROWTH FACTOR

Authors

  • FAIZATUN Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta-12640, Indonesia https://orcid.org/0000-0001-5761-1118
  • ABUL A’LA MAUDUDI Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta-12640, Indonesia
  • NUR MIFTAHURROHMAH Faculty of Pharmacy, Universitas Pancasila, South Jakarta, DKI Jakarta-12640, Indonesia
  • DIAH KARTIKA PRATAMI Lab of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta-12640, Indonesia. Center for Study of Natural Product for Degenerative Disease, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta-12640, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s3.04

Keywords:

Morus alba l., Callus leaf, Tyrosinase inhibitor, Skin lightening, Nanostructured lipid carrier

Abstract

Objective: This research aimed to formulate the callus extract of mulberry leaf in the form of a Nanostructured lipid Carrier (NLC) gel.

Methods: Dichlorophenoxyacetic acid (2,4–D) and Benzyl Amino Purine (BAP) was used as a callus growth factor. Callus leaf extracted with ethanol 96% by maceration-sonication method. An amount of 0.5% callus leaf extract was formulated into NLC. The NLC is then evaluated for its particle size and polydispersity index. The NLC gel is evaluated for its organoleptic, homogeneity, viscosity, flow ability, and pH. The callus extract and the NLC gel were also evaluated for their tyrosinase inhibitor activity.

Results: The best formulation of NLC showed a particle size of 189.8 nm and a polydispersity index of 0.578. The NLC is a semi-solid, yellowish, odorless, homogeneous gel, with viscosity of 26,666.67 cPs, plastic-thixotropic type, pH of 5.26. The evaluation of tyrosinase inhibitory activity of the callus extract and the NLC gel showed IC50 value of 217.64 and 248.12 ug/ml.

Conclusion: It can be concluded that leaf callus extract of mulberry can be formulated into an NLC gel that is physically and chemically stable and has good skin-lightening activity.

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References

Sorokina M, Steinbeck C. Review on natural products databases: where to find data in 2020. J Cheminform. 2020;12(1):20. doi: 10.1186/s13321-020-00424-9, PMID 33431011.

Karuppusamy S. A review on trends of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plants Res. 2009;3(13):1222-39.

Trivedi M, Singh A, Johri P, Singh R, Tiwari RK. Genetic engineering potential of hairy roots of poppy (Papaver spp.) for production of secondary metabolites, phytochemistry, and in silico approaches. Biotechnol Approaches Med Aromat Plants Conserv Genet Improv Util. 2018:569-97.

Bidabadi SS, Jain SM. Cellular, molecular, and physiological aspects of in vitro plant regeneration. Plants (Basel). 2020;9(6):702. doi: 10.3390/plants9060702, PMID 32492786.

Zhang R, Zhang Q, Zhu S, liu B, liu F, Xu Y. Mulberry leaf (Morus alba l.): a review of its potential influences in mechanisms of action on metabolic diseases. Pharmacol Res. 2022;175:106029. doi: 10.1016/j.phrs.2021.106029, PMID 34896248.

Thassu D, Pathak Y, Deleers M. Nanoparticulate drug-delivery systems: an overview. Drugs Pharm Sci. 2007;166:1-31. doi: 10.1201/9781420008449.ch1.

Faizatun F, Murti II. Formulation of nanostructured lipid carrier gel from mulberry root extract (Morus alba l.) as whitening agent using zebrafish modelling. Indonesian J Pharm Sci. 2023;21(2):209-14. doi: 10.35814/jifi.v21i2.1473.

Nagamani DB, Reddy KB, Maheswaramma KS, Raj SB. Implementation of factorial design to optimize the formulation method of ezetimibe polymeric nanoparticle by homogenization cum ultra-sonication method. Int J App Pharm. 2022;14(2):151-9. doi: 10.22159/ijap.2022v14i2.43783.

Sangsen Y, Likhitwitayawuid K, Sritularak B, Wiwattanawongsa K, Wiwattanapatapee R. Novel solid lipid nanoparticles for oral delivery of oxyresveratrol: effect of the formulation parameters on the physicochemical properties and in vitro release. Int J Med Pharm Sci Eng. 2013;7(12):506-13.

Maulia E, Basyah B. Growth of patchouli shoots (Pogostemon cablin Benth.) with several concentrations of growth regulator substances in vitro. J Agric Vet Sci. 2021;14(1):38-46.

Campos Boza S, Vinas M, Solorzano Cascante P, Holst A, Steinmacher DA, Guerra MP. Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots. Front Plant Sci. 2022;13:995307. doi: 10.3389/fpls.2022.995307, PMID 36247585.

Klimek Chodacka M, Kadluczka D, lukasiewicz A, Malec Pala A, Baranski R, Grzebelus E. Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena l. Plant Cell Tissue Organ Cult (PCTOC). 2020;143(3):693-707. doi: 10.1007/s11240-020-01953-9.

Kang S, Yoon TW, Kim GY, Kang B. Review of conjugated polymer nanoparticles: from formulation to applications. ACS Appl Nano Mater. 2022;5(12):17436-60. doi: 10.1021/acsanm.2c04730.

Hassan DH, Shohdy JN, El-Nabarawi M, El-Setouhy DA, Abdellatif MM. Nanostructured lipid carriers for transdermal drug delivery. Int J App Pharm. 2022;14(4):88-93. doi: 10.22159/ijap.2022v14i4.44564.

Gordillo Galeano A, Mora Huertas CE. Hydrodynamic diameter and zeta potential of nanostructured lipid carriers: emphasizing some parameters for correct measurements. Colloids Surf A Physicochem Eng Aspects. 2021;620:126610. doi: 10.1016/j.colsurfa.2021.126610.

Noor SU, Laksmitawati DR, Sumaryono W. In vitro antihyaluronidase activity and physical properties of Alpinia zerumbet rhizome extract-loaded nanostructured lipid carrier. J Ilmu Kefarmasian Indones. 2020;18(1):48-52.

Tanuwidjaja T. Development of anti-aging cream preparations with active substances from plant extracts: physicochemical review and potential applications. J Eduhealth. 2023;14(3):1310-25. doi: 10.54209/jurnaleduhealth.v14i3.2522.

Thamrongwatwongsa J, Pattarapipatkul N, Jaithon T, Jindaruk A, Paemanee A, T-Thienprasert NP. Mulberroside F from in vitro culture of mulberry and the potential use of the root extracts in cosmeceutical applications. Plants. 2022;12(1):146. doi: 10.3390/plants12010146.

Gomes D, Sousa A, Passarinha LA. Tyrosinase immobilization in nickel-cross-linked gellan microspheres and conversion of l -DOPA to dopachrome. J Chem Educ. 2021;98(11):3560-6. doi: 10.1021/acs.jchemed.1c00176.

Lee ON, Ak G, Zengin G, Cziaky Z, Jeko J, Rengasamy KR. Phytochemical composition, antioxidant capacity, and enzyme inhibitory activity in callus, somaclonal variant, and normal green shoot tissues of Catharanthus roseus (L) G. Don. Molecules. 2020;25(21):4945. doi: 10.3390/molecules25214945, PMID 33114628.

Published

12-08-2024

How to Cite

FAIZATUN, MAUDUDI, A. A., MIFTAHURROHMAH, N., & PRATAMI, D. K. (2024). FORMULATION OF NANOSTRUCTURED LIPID CARRIER GEL FROM CALLUS EXTRACT OF MULBERRY LEAF (MORUS ALBA L.) WITH 2, 4-DICHLORO PHENOXY ACETIC ACID AND BENZYL AMINO PURINE AS CALLUS GROWTH FACTOR. International Journal of Applied Pharmaceutics, 16(3), 22–27. https://doi.org/10.22159/ijap.2024.v16s3.04

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