ANALYSIS OF PROPOLIS STINGLESS BEE BIOACTIVE COMPOUNDS FROM SEVERAL REGIONS IN INDONESIA

DIAH KARTIKA PRATAMI; SANIA CITRA ALFIFAH; IZZUL ISLAM; MUHAMAD SAHLAN; SRI ANGKY SOEKANTO

doi:10.22159/ijap.2024v16i3.14

Authors

DIAH KARTIKA PRATAMI Lab of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta-12640, Indonesia. Center for Study of Natural Product for Degenerative Disease, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta-12640, Indonesia https://orcid.org/0000-0003-1052-5946
SANIA CITRA ALFIFAH Faculty of life Sciences and Technology, Sumbawa University of Technology, Sumbawa, West Nusa Tenggara-84371, Indonesia
IZZUL ISLAM Faculty of life Sciences and Technology, Sumbawa University of Technology, Sumbawa, West Nusa Tenggara-84371, Indonesia
MUHAMAD SAHLAN Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java-16424, Indonesia. Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java-16424, Indonesia
SRI ANGKY SOEKANTO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Central Jakarta, DKI Jakarta-10430, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i3.14

Keywords:

Stingless bee, Propolis, lC-MS/MS, Phenolic, Flavonoid, Antioxidant

Abstract

Objective: Propolis is a bee product collected by honeybees from a resinous substance of various plant sources. Its antioxidant activities are different from various geographic origins. This study aimed to analyze bioactive compounds by lC-MS/MS, compare antioxidant activity total phenolic and flavonoid contents in stingless bee propolis samples from several regions in Indonesia.

Methods: The propolis samples were taken from stingless bee hives of Tetragonula clypearis from Sumbawa, Tetragonula laeviceps from Magelang, Tetragonula biroi from Bogor, and Geniotrigona thoracica from South Kalimantan). Analysis of bioactive compounds was identified by lC-MS/MS. The quantification of the chemical compound determined its total phenolics and flavonoid (TPC and TFC) contents. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay.

Results: The results of this study obtained 10 compounds from G. thoracica, 8 compounds each from T. laeviceps and T. biroi, and 7 compounds from T. clypearis. The results of TPC was ranging from 57.24±5.35 to 139.39±15.79 mg GAE/g. The TFC was ranging from 22.13±0.79 to 37.20±3.63 mg QE/g. With the highest TPC and TFC was propolis of G. thoracica. The IC₅₀ of antioxidant activity was ranging from 11.12 to 162.72 ppm.

Conclusion: The compounds contained in propolis T. clypearis, T. laeviceps, G. thoracica and T. biroi have a potential as a new herbal candidate as antioxidant agents.

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References

Bankova V, Popova M, Trusheva B. The phytochemistry of the honeybee. Phytochemistry. 2018 Nov;155:1-11. doi: 10.1016/j.phytochem.2018.07.007, PMID 30053651.

Balica G, Vostinaru O, Stefanescu C, Mogosan C, Iaru I, Cristina A. Potential role of propolis in the prevention and treatment of metabolic diseases. Plants (Basel). 2021;10(5):1-14. doi: 10.3390/plants10050883, PMID 33925692.

De Groot AC. Propolis: a review of properties, applications, chemical composition, contact allergy, and other adverse effects. Dermatitis. 2013;24(6):263-82. doi: 10.1097/DER.0000000000000011, PMID 24201459.

Farida S, Pratami DK, Sahlan M, Mun’im A, Djamil R, Winarti W. In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens. J King Saud Univ Sci. 2023;35(6):102738. doi: 10.1016/j.jksus.2023.102738.

Pratami DK, Mun’im A, Sundowo A, Sahlan M. Phytochemical profile and antioxidant activity of propolis ethanolic extract from tetragonula bee. Pharmacogn J. 2017;10(1):128-35. doi: 10.5530/pj.2018.1.23.

Pratami DK, Desmiaty Y, Simorangkir EM, Faradhila D. Standardization and antioxidant activity of propolis extract for SARS-CoV2 infection therapy. J Ilmu Kefarmasian Indones. 2021;19(2):272-80.

Pratami DK, Indrawati T, Istikomah I, Farida S, Pujianto P, Sahlan M. Antifungal activity of microcapsule propolis from Tetragonula spp. to candida albicans. Commun Sci Technol. 2020;5(1):16-21. doi: 10.21924/cst.5.1.2020.178.

Pratami DK, Mun’Im A, Yohda M, Hermansyah H, Gozan M, Putri YR. Total phenolic content and antioxidant activity of spray-dried microcapsules propolis from Tetragonula species. AIP Conf Proc. 2019. doi: 10.1063/1.5095018.

Kumazawa S, Hamasaka T, Nakayama T. Antioxidant activity of propolis of various geographic origins. Food Chem. 2004;84(3):329-39. doi: 10.1016/S0308-8146(03)00216-4.

Bankova V, Trusheva B, Popova M. Propolis extraction methods: a review. J Apic Res. 2021;60(5):734-43. doi: 10.1080/00218839.2021.1901426.

Anđelkovic B, Vujisic L, Vuckovic I, Tesevic V, Vajs V, Godevac D. Metabolomics study of Populus type propolis. J Pharm Biomed Anal. 2017;135:217-26. doi: 10.1016/j.jpba.2016.12.003, PMID 28012592.

Segueni N, Zellagui A, Moussaoui F, Lahouel M, Rhouati S. Flavonoids from Algerian propolis. Arab J Chem. 2016;9:S425-8. doi: 10.1016/j.arabjc.2011.05.013.

Pratami DK, Mun’im A, Hermansyah H, Gozan M, Sahlan M. Microencapsulation optimization of propolis ethanolic extract from Tetragonula spp using response surface methodology. Int J App Pharm. 2020;12(1):197-206. doi: 10.22159/ijap.2020v12i4.37808.

Qodriah R, Kumala S, Syamsudin S, Yuliana ND, Simanjuntak P, Putri E. Identification of antioxidant compounds in fig. leaves (Ficus carica l) fractions using lC-MS/MS. Sci Pharm. 2023;2(4):12-21.

Treml J, Smejkal K. Flavonoids as potent scavengers of hydroxyl radicals. Compr Rev Food Sci Food Saf. 2016;15(4):720-38. doi: 10.1111/1541-4337.12204, PMID 33401843.

Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hara H. Comparison of bee products based on assays of antioxidant capacities. BMC Complement Altern Med. 2009;9:4. doi: 10.1186/1472-6882-9-4, PMID 19243635.

Spicher L, Kessler F. Unexpected roles of plastoglobules (plastid lipid droplets) in vitamin K1 and E metabolism. Curr Opin Plant Biol. 2015 Jun 1;25:123-9. doi: 10.1016/j.pbi.2015.05.005, PMID 26037391.

Cao X, Wu L, Wu M, Zhu C, Jin Q, Zhang J. Abscisic acid-mediated proline biosynthesis and antioxidant ability in roots of two different rice genotypes under hypoxic stress. BMC Plant Biol. 2020 Dec;20(1):198. doi: 10.1186/s12870-020-02414-3, PMID 32384870.

Zhang J, Powell C, Meruvu S, Sonkar R, Choudhury M. Pyrroloquinoline quinone attenuated benzyl butyl phthalate induced metabolic aberration and a hepatic metabolomic analysis. Biochem Pharmacol. 2022 Mar 1;197:114883. doi: 10.1016/j.bcp.2021.114883, PMID 34971587.

Xiao X, Erukainure OL, Sanni O, Koorbanally NA, Islam MS. Phytochemical properties of black tea (Camellia sinensis) and rooibos tea (Aspalathus linearis); and their modulatory effects on key hyperglycaemic processes and oxidative stress. J Food Sci Technol. 2020 Dec;57(12):4345-54. doi: 10.1007/s13197-020-04471-w, PMID 33087948.

Silva FM, Ferreira ML, Cavalcante Lucena JE, Bicudo AJ. DL-carnitine as supplementary levocarnitine source in plant-based diets to Nile tilapia (Oreochromis niloticus) fingerlings. Lat Am J Aquat Res. 2018 Sep;46(4):825-30.

Alirezaei M, Khoshdel Z, Dezfoulian O, Rashidipour M, Taghadosi V. Beneficial antioxidant properties of betaine against oxidative stress mediated by levodopa/Benserazide in the brain of rats. J Physiol Sci. 2015 May;65(3):243-52. doi: 10.1007/s12576-015-0360-0, PMID 25665954.

Babaoğlu Aydaş SB, Ozturk S, Aslım B. Phenylalanine ammonia-lyase (PAL) enzyme activity and antioxidant properties of some cyanobacteria isolates. Food Chem. 2013;136(1):164-9. doi: 10.1016/j.foodchem.2012.07.119, PMID 23017408.

Akgul Y, Yildiz Aktas L, Anil H. Compounds from flowers of Daucus carota l. SSP. Carota and their antioxidant activity. Chem Nat Compd. 2009;45(6):889-92. doi: 10.1007/s10600-010-9479-7.