ANTIOXIDANT ACTIVITY OF FLAVONOID RICH FRACTION OF (VERNONIA AMYGDALINA DELILE.) LEAVES

VIONA ROSALINA; POPPY ANJELISA ZAITUN  HASIBUAN; DENNY SATRIA; EDY MEIYANTO; DEDDI PRIMA  PUTRA; MORALITA CHATRI; ENDAH PUJI SEPTISETYANI

doi:10.22159/ijap.2024v16s4.52249

Authors

VIONA ROSALINA Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Sumatera Utara-20155, Indonesia https://orcid.org/0009-0007-8361-193X
POPPY ANJELISA ZAITUN HASIBUAN Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Sumatera Utara-20155, Indonesia
DENNY SATRIA Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Sumatera Utara-20155, Indonesia https://orcid.org/0000-0003-4724-3256
EDY MEIYANTO Laboratory of Macromolecular Engineering, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, Yogyakarta-55281, Indonesia
DEDDI PRIMA PUTRA Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera-25163, Indonesia https://orcid.org/0000-0002-0930-3150
MORALITA CHATRI Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, West Sumatera-25131, Indonesia
ENDAH PUJI SEPTISETYANI Research Center for Genetic Engineering, National Research and Innovation Agency-16911, Indonesia https://orcid.org/0000-0002-6764-9686

DOI:

https://doi.org/10.22159/ijap.2024v16s4.52249

Keywords:

African leaves, Vernonia amygdalina del., Antioxidant, Flavonoid rich fraction

Abstract

Objective: Atoms that are unstable and harmful to other molecules are referred to as free radicals. Flavonoid molecules have the ability to act as antioxidants, which can help avoid oxidative stress brought on by the high levels of free radicals that are found in the body. Antioxidants are chemicals that bind to free radicals and neutralize oxidized substances by giving electrons. The main objective of this comprehensive investigation was to assess and quantify the extent of antioxidant activity manifested by fractions abundant in flavonoids extracted from Vernonia amygdalina Del.

Methods: Refluxing n-hexane solvent and then continuing with methanol were the steps that taken in the process of extracting Vernonia amygdalina. The methanol extract was subjected to fractionation utilizing the liquid-liquid fractionation method, employing a series of solvents to isolate distinct fractions with varying chemical compositions. After determining the overall flavonoid content of each extract and fraction, an evaluation was conducted on the antioxidant activity of the samples containing the maximum flavonoid content.

Results: The dichlormethane fraction had the highest flavonoid content with 69.56±0.22 mg/g QE. Then the DCM fraction was tested for antioxidant activity, and the results obtained for the DPPH method were 117.33±0.56 and the CUPRAC method was was 84.80±0.73.

Conclusion: The results showed that DCM fraction is the highest flavonoid fraction and exhibits potent antioxidant properties.

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References

Muflihah YM, Gollavelli G, Ling YC. Correlation study of antioxidant activity with phenolic and flavonoid compounds in 12 Indonesian indigenous herbs. Antioxidants (Basel). 2021;10(10):1530. doi: 10.3390/antiox10101530, PMID 34679665.

Rahmi H. Review: aktivitas antioksidan dari berbagai sumber buah-buahan di Indonesia. J Agrotek Indones. 2017;2(1):721. doi: 10.33661/jai.v2i1.721.

Werdhasari A, Kesehatan PAB. J Biotekhnol Medisiana Indones. 2014;3(1).

Satria D, Harahap U, Dalimunthe A, Septama AW, Hertiani T, Nasri N. Synergistic antibacterial effect of ethyl acetate fraction of Vernonia amygdalina delile leaves with tetracycline against clinical isolate methicillin-resistant ¬Staphhylococcus aureus (MRSA) and Pseudomonas aeruginosa. Hindawi Adv Pharmacol Pharm Sci. 2023;2:1-11.

Satria D. The effect of extraction method of Vernonia amygdalina delile. Leaves Cardiotonic Effect Farm. 2023;71(2):393.

Syahputra RA, Harahap U, Dalimunthe A, Nasution P, Haro G, Widodo. In-silico toxicity prediction of bioactive compounds of vernonia amygdalina delile and digoxin. Rasayan J Chem. 2020;13(2):1220-4. doi: 10.31788/RJC.2020.1325638.

Wang WT, Liao SF, Wu ZL, Chang CW, Wu JY. Simulataneous study if antioxidant activity, DNA protection and anti-inflammatory effect of Vernonia amygdalina Leaves extracts. Plos One. 2021;15(7):e0235717.

Harahap U, Dalimunthe A, Hertiani T, Muhammad M, Nasri S, Satria D. Antioxidant and antibacterial activities of ethanol extract of Vernonia amygdalina delile leaves. AIP Conference Proceedings. 2021;1. doi: 10.1063/5.0045447.

Slika H, Mansour H, Wehbe N, Nasser SA, Iratni R, Nasrallah G. Therapeutic potential of flavonoids in cancer: ROS-mediated mechanisms. Biomed Pharmacother. 2022;146:112442. doi: 10.1016/j.biopha.2021.112442, PMID 35062053.

Kusuma WSA. The effect of ethanol extract of soursop leaves (Annona muricata L.) to decreased levels of malondialdehyde. J Majority. 2015;4(3).

Shraim AM, Ahmed TA, Rahman MM, Hijji YM. Determination of total flavonoid content by aluminium chloride assay: a critical evaluation. Food Sci Technol. 2021;150.

Phuyal N, Jha PK, Raturi PP, Rajbhandary S. Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of zanthoxylum armatum DC. Scientific World Journal. 2020;2020:8780704. doi: 10.1155/2020/8780704, PMID 32256249.

Sayakti PI, Anisa N, Ramadhan H. Antioxidant activity of methanol extract of cassava leaves (Manihot esculenta Crants) using CUPRAC method. Sci J Iof Pharm. 2022;97.

Ogidi OI, George DG, Esie NG. Ethnopharmacological properties of Vernonia amygdalina (Bitter Leave) medical pant. J Med Plants Stud. 2019;7(2):179.

Molyneux P. The use of the stable free radical Diphenyl Picrylhydrazyl (DPPH) for estimating antioxidant activity. J Sci Technol. 2004;26(2):211-9.

Harborne JB, Fitokimia M. Bandung: ITB; 1987.

Azizah DN, Kumolowati E, Faramayuda F. Flavonoid metode AlCl3 Pada ekstrak metanol kulit buah Kakao (Theobroma cacao L.). Kartika J Ilmiah Farmasi. 2014;2(2):45-9. doi: 10.26874/kjif.v2i2.14.

Suhendy H, Alif A, Rahmiyani I. korelasi kadar fenolik dan flavonoid total terhadap aktivitas antioksidan beberapa ekstrak daun afrika (Vernonia amygdaliina Delile.) menggunakan metode FRAP. Med Sains J Ilmiah Kefarmasian. 2022;7(2):225.

Anwar K, Triyasmono L, Fenolik KT. Total flavonoid, dan aktivitas antioksidan ekstrak etanol buah mengkudu (Morinda citrifolia L.). J Pharmascience. 2016;3(1):83-92.

Ugbogu EA, Emmanuel O, Dike ED, Agi GO, Ugbogu OC, Ibe C. The phytochemistry, ethnobotanical, and pharmacological potentials of the medicinal plant-Vernonia amygdalina L. (bitter leaf). Clinical Complementary Medicine and Pharmacology. 2021;1(1). doi: 10.1016/j.ccmp.2021.100006.

Cepeda GN, Murtiningrum W, Kapasitas Stabilitas Y. Radikal bebas dan kelas ion metal ekstrak air daun Vernonia amygdalina del. Agritechnology. 2020;3(2):79.

Nugraha AT, Firmansyah MS, Jumaryatno P. Profil senyawa dan aktivitas antioksidan daun yakon (Smallanthus sonchifolius) dengan metode DPPH dan CUPRAC. J Ilmiah Farmasi. 2017;13(1):14.

Sadeer BN, Montesano D, Albrizio S, Zengin G, Mahomoodally F. The versatility of antioxidant assays in food science and safety chemistry, applications, strength, and limitations. MDPI; 2020. p. 9.

Fatimah N, Anf Sundu R. Uji aktivitas antioksidan fraksi n-heksan daun afrika (Vernonia amygdalina Del.) dengan metode DPPH. J Ilmiah Ibnu Sina. 2020;5(2):250-3.

Maryam S, Pratama R, Effendi N, Naid T. Analisis aktivitas antioksidan ekstrak etanolik daun yodium (Jatropha multifida L.) dengan metode cupric ion reducing antioxidant capacity (cuprac). J Fitofarmaka Indones. 2016;2(1). doi: 10.33096/jffi.v2i1.185.

Muhammad M, Putra ED, Cintya H, Satria D. The effect of solvent towards antioxidant activity of Vernonia amygdalina delile leaves. Rasayan J Chem. 2023;16(2):760-5. doi: 10.31788/RJC.2023.1628059.

Tunasamy K, Suryadevara N, Athimoolam T. Screening of Vernonia amygdalina leaf extracts for antioxidant and antimicrobial activity. Sci Direct Mater Todal Proceeding. 2019;16:1809.

Alara OR, Abdurahman NH, Olalere OA. Ethanolic extraction of flavonoids, phenolics and antioxidants from Vernonia amygdalina leaf using two-level factorial design. Journal of King Saud University-Science. 2020;32(1):7-16. doi: 10.1016/j.jksus.2017.08.001.