ANTIINFLAMMATION AND ANTIFUNGAL EFFECTS OF SILVER NANOPARTICLES GREENLY SYNTHESIZED USING PHYLLANTHUS EMBLICA L. EXTRACT AS A REDUCING AGENT AGAINST DERMATOPHYTOSIS

MASFRIA; SUMAIYAH; HAFID SYAHPUTRA; VERENTIO FANI

doi:10.22159/ijap.2024v16s4.52254

Authors

MASFRIA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia. Nanomedicine Center of Innovation, Universitas Sumatera Utara, Medan, Indonesia
SUMAIYAH Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia. Nanomedicine Center of Innovation, Universitas Sumatera Utara, Medan, Indonesia
HAFID SYAHPUTRA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
VERENTIO FANI Undergraduate Student, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16s4.52254

Keywords:

Silver nanoparticles, Green synthesis, Phyllanthus emblica, Anti-inflammatory, Antifungal

Abstract

Objective: This research aims to determine whether the extract of Phyllanthus emblica can synthesize silver nanoparticles as an alternative bioreductive synthesis that is safer and free of hazardous waste. Silver nanoparticles were tested for their anti-inflammatory and antifungal activity on Trichophyton mentagrophytes and Microsporum canis.

Methods: This study makes ethanol extract of Phyllanthus emblica fruit, synthesizes silver nanoparticles utilizing green synthesis, and then characterizes and tests for anti-inflammatory and antifungal activities.

Results: This research shows that Phyllanthus emblica ethanol extract can be used to produce silver nanoparticles with characterization using a UV-Vis spectrophotometer at 430 nm, and FTIR shows specific wave numbers at 3739, 1703, 1347, 1054 and 875 cm-1. Particle Size Analyzer (PSA) and Scanning Electron Microscope (SEM) analysis of silver nanoparticles showed a colloid size of 25.93 nm and dry silver nanoparticle powder of 112.68 nm with spherical particles and nanometer scale size. In anti-inflammatory activity with a weak IC₅₀ and optimal antifungal activity was seen at concentrations of Trichophyton mentagrophytes (125 mg/ml) and Microsporum canis (150 mg/ml).

Conclusion: The ethanol extract of Phyllanthus emblica fruit produced silver nanoparticles with anti-inflammatory and antifungal properties.

Downloads

Download data is not yet available.

References

Ahmad B, Hafeez N, Rauf A, Bashir S, Linfang H, Rehman M. Phyllanthus emblica: a comprehensive review of its therapeutic benefits. S Afr J Bot. 2021;138:278-310. doi: 10.1016/j.sajb.2020.12.028.

Shafey AM. Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: a review. Green Process Synth. 2020;9(1):304-39. doi: 10.1515/gps-2020-0031.

Simangunsong TL, Putra A, Ginting SF, Wardhani FM, Abdullah H, Ikhtiari R. Antifungal analysis of papaya seed extracts and biosynthesized silver nanoparticles. Sci Technol Indones. 2022;7(2):186-94. doi: 10.26554/sti.2022.7.2.186-194.

Septifani EA, Yetti RD, Asra R. Review: the discovery and development of sildenafil citrate. Asian J Pharm Res Dev. 2021;9(4):108-17. doi: 10.22270/ajprd.v9i4.1018.

Costa JE, Neves RP, Delgado MM, Lima Neto RG, Morais VM, Coelho MR. Dermatophytosis in patients with human immunodeficiency virus infection: clinical aspects and etiologic agents. Acta Trop. 2015;150:111-5. doi: 10.1016/j.actatropica.2015.07.012, PMID 26200786.

Depkes RI. Farmakope herbal Indonesia; 2008.

Shah PJ, Malik R. Study of antibacterial activity of Phyllanthus emblica and its role in green synthesis of silver nanoparticles. J Drug Delivery Ther. 2019;9(3):76-81. doi: 10.22270/jddt.v9i3.2753.

Masum MM, Siddiqa MM, Ali KA, Zhang Y, Abdallah Y, Ibrahim E. Biogenic synthesis of silver nanoparticles using phyllanthus emblica fruit extract and its inhibitory action against the pathogen acidovorax oryzae strain RS-2 of rice bacterial brown stripe. Front Microbiol. 2019;10:820. doi: 10.3389/fmicb.2019.00820, PMID 31110495.

Ghoshal KP. Green synthesis of silver nano particles derieved from leaf extract of syzygiumcuminii (SNSC)-to evaluate antibacterial activity. IJARSCT. 2021;12(4):384-9. doi: 10.48175/IJARSCT-2405.

Taba P, Parmitha NY, Kasim S. Synthesis of silver nanoparticles using syzygium polyanthum extract as bioreductor and the application as antioxidant. Indonesian J Chem Res. 2019;7(1):51-60.

Maurya AK, Agarwal K, Gupta AC, Saxena A, Nooreen Z, Tandon S. Synthesis of eugenol derivatives and its anti-inflammatory activity against skin inflammation. Nat Prod Res. 2020;34(2):251-60. doi: 10.1080/14786419.2018.1528585, PMID 30580605.

Singla S, Jana A, Thakur R, Kumari C, Goyal S, Pradhan J. Green synthesis of silver nanoparticles using Oxalis griffithii extract and assessing their antimicrobial activity. Open Nano. 2022;7:2022.100047. doi: 10.1016/j.onano.2022.100047.

Abdelsattar AS, Kamel AG, El-Shibiny A. The green production of eco-friendly silver with cobalt ferrite nanocomposite using Citrus limon extract. Results in Chemistry. 2023;5. doi: 10.1016/j.rechem.2022.100687.

Gupta SM, Arif M, Ahmed Z. Antimicrobial activity in leaf, seed extract and seed oil of Jatropha curcas L. plant. J Appl Nat Sci. 2011;3(1):102-5. doi: 10.31018/jans.v3i1.164.

Yin IX, Zhang J, Zhao IS, Mei ML, Li Q, Chu CH. The antibacterial mechanism of silver nanoparticles and its application in dentistry. Int J Nanomedicine. 2020;15:2555-62. doi: 10.2147/IJN.S246764, PMID 32368040.