CHARACTERIZATION OF ISOLATED CRYSTALS FROM MANGROVE LEAVES (AVICENNIA MARINA AND SONNERATIA ALBA) AND THEIR ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS

LINA PERMATASARI; HANDA MULIASARI; FANIA RAHMAN

doi:10.22159/ijap.2024v16s5.52480

Authors

LINA PERMATASARI 1Department of Pharmaceutical Chemistry, Study Program of Pharmacy, Faculty of Medicine and Health Sciences, University of Mataram, Mataram-83115, Indonesia https://orcid.org/0000-0002-4214-9124
HANDA MULIASARI Department of Pharmaceutical Chemistry, Study Program of Pharmacy, Faculty of Medicine and Health Sciences, University of Mataram, Mataram-83115, Indonesia
FANIA RAHMAN Department of Pharmaceutical Chemistry, Study Program of Pharmacy, Faculty of Medicine and Health Sciences, University of Mataram, Mataram-83115, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16s5.52480

Keywords:

Avicennia marina, Sonneratia alba, Mangroves, Staphylococcus aureus, Isolated crystal, Antibacterial activity

Abstract

Objective: The study aimed to characterize the isolated crystal of Avicennia marina (A. marina) and Sonneratia alba (S. alba) leaves and identify the antibacterial activity of their isolated crystal.

Methods: Each of the leaves A. marina and S. alba was extracted using ethanol 96%, then fractionated by liquid-liquid extraction method using n-hexane and ethyl acetate. The crystal of the isolated crystal was found in the water fraction. The isolated crystals were characterized using Fourier Transform-Infrared (FT-IR) and Scanning Electron Microscope-Energy Dispersive X-ray (SEM-EDX) Spectroscopy and identified the antibacterial activity against Staphylococcus aureus (S. aureus) using the diffusion method.

Results: The FT-IR spectrum showed that the isolated crystal was a polysaccharide structure with vibrations in the O-H, C=O, C-O, C-H, and S=O bonds. The SEM-EDX spectrum revealed the high-level content of carbon and oxygen with sulfuryl group proposing the sulfated polysaccharide compound. Water fraction of A. marina and S. alba have inhibition zones 16 mm and 10 mm, respectively. The results showed the water fraction of A. marina and S. alba have strong and moderate antibacterial activity, respectively. Meanwhile, the antibacterial activity of the isolated crystal was none. The isolated crystal was estimated a sulfated polysaccharide but was not pure. So that the antibacterial activity was not detected.

Conclusion: Even though, the water fraction of A. marina and S. alba leaves can be developed as antibacterial promising.

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References

. Abeysinghe PD. Antibacterial Activity of some Medicinal Mangroves against Antibiotic Resistant Pathogenic Bacteria. Indian J Pharm Sci. 2010;72(2):167–72.

. Lee YS, Lee DY, Kim YB, Lee SW, Cha SW, Park HW, et al. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evid Based Complement Alternat Med. 2015 Jul 21;2015(7): 1-7.

. Sahoo G, Mulla NSS, Ansari ZA, Mohandass C. Antibacterial Activity of Mangrove Leaf Extracts against Human Pathogens. Indian J Pharm Sci. 2012;74(4):348–51.

. Tresiana N, Duadji N, Febryano IG, Zenitha SA. Saving Mangrove Forest Extinction in Urban Areas: Will Government Interventions Help? Int J Sustain Dev Plan. 2022 Apr 26;17(2):75–84.

. Giesen W, Wulfraat S, Zieren M, Scholten L. Mangrove Guidebook for Southeast Asia [Internet]. 1st ed. Netherlands: FAO Regional Office for Asia and the Pacific (RAP); 2017 [cited 2023 Jan 5]. Available from: https://www.fao.org/3/ag132e/ag132e00.pdf

. Saranraj P. Mangrove Medicinal Plants: A Review. Am-Eurasian J Toxicol Sci. 2015 Jan 1;7:146–56.

. Al-Mur B. Biological Activities of Avicennia marina Roots and Leaves Regarding Their Chemical Constituents. Arab J Sci Eng. 2021 Jan 6;46(6):1–13.

. Behbahani BA, Yazdi FT, Shahidi F, Riazi F. Antifungal Effect of the Aqueous and Ethanolic Avicennia marina Extracts on Alternaria citri and Penicillium digitatum. Zahedan J Res Med Sci [Internet]. 2016 [cited 2022 Jul 11];18(2). Available from: https://brieflands.com/articles/zjrms-5992.html#abstract

. Das L, Bhaumik E, Raychaudhuri U, Chakraborty R. Role of nutraceuticals in human health. J Food Sci Technol. 2012 Apr 1;49(2):173–183.

. Kaur N, Kumar V, Nayak SK, Wadhwa P, Kaur P, Sahu SK. Alpha-amylase as molecular target for treatment of diabetes mellitus: A comprehensive review. Chem Biol Drug Des. 2021;98(4):539–560.

. Okla MK, Alatar AA, Al-amri SS, Soufan WH, Ahmad A, Abdel-Maksoud MA. Antibacterial and Antifungal Activity of the Extracts of Different Parts of Avicennia marina (Forssk.) Vierh. Plants. 2021 Jan 28;10(2): 1-14.

. Wijaya MD, Indraningrat AAG. Antibacterial Activity of Mangrove Root Extracts from Ngurah Rai Mangrove Forest, Denpasar-Bali. Biol Med Nat Prod Chem. 2021 Oct 21;10(2):117–121.

. Rahmania N. Phytochemical Test of Mangrove Avicennia alba, Rhizophora apiculata and Sonneratia alba from Musi River Estuary, South Sumatera. Biovalenta Biol Reseach J. 2018;4(2):8–15.

. Thatoi H, Samantaray D, Das SK. The genus Avicennia , a pioneer group of dominant mangrove plant species with potential medicinal values: a review. Front Life Sci. 2016 Oct;9(4):267–291.

. Syahidah, Subekti N. Biological Activity of Mangrove Leaves Extract (Rhizophora sp.). IOP Conf Ser Earth Environ Sci. 2019 May 1;270(1): 1-8.

. Muhaimin M, latifah N, Chaerunnisa AY, Amalia E, Rostinawati T. Preparation And Characterization Of Sonneratia Alba Leaf Extract Microcapsules By Solvent Evaporation Technique. Int J Appl Pharm. 2022;14(6):77–82.

. Trivedi H, Puranik PK. Antibacterial Activity Of Chlorogenic Acid Phytovesicles Against Resistant Bacteria: Development, Optimization And Evaluation. Int J Appl Pharm. 2022;14(1):83–92.

. Essa HL, Guirguis HA, El-Sayed MMH, Rifaat D, Abdelfattah MS. Ultrasonically-Extracted Marine Polysaccharides as Potential Green Antioxidant Alternatives. In: The 1st International Electronic Conference on Applied Sciences [Internet]. MDPI; 2020 [cited 2023 Jan 4]. p. 23. Available from: https://www.mdpi.com/2504-3900/67/1/23

. Latief M, Utami A, Amanda H, Muhaimin, Afifah Z. Antioxidant activity of isolated compound from perepat roots (Sonneratia alba). J Phys Conf Ser. 2019 Jul;1282(1): 1-7.

. Harizon, Pujiastuti B, Kurnia D, Sumiarsa D, Shiono Y, Supratman U. Antibacterial Triterpenoids from the Bark of Sonneratia alba (Lythraceae). Nat Prod Commun. 2015 Feb 1;10(2):277-280.

. Musa WJA, Bialangi N, Situmeang B, Silaban S. Triterpenoid compound from metanol extract of mangrove leaves (Sonneratia alba) and anti-cholesterol activity test. J Pendidik Kim JPKIM. 2019 Apr 30;11(1):18–23.

. Wang Q, Zhou X, Gou H, Chang H, Lan J, Li J, et al. Antibacterial activity of a polysaccharide isolated from Artemisia argyi leaf against Staphylococcus aureus and mechanism investigation. Int J Biol Macromol. 2023 Dec 31;253(1):1-9.

. Mao JW, Yin J, Ge Q, Jiang ZL, Gong J. In vitro antioxidant activities of polysaccharides extracted from Moso Bamboo-Leaf. Int J Biol Macromol. 2013 Jan 7;55(1):1-5.

. Kondakova AN, Senchenkova SN, Gremyakov AI, Shashkov AS, Knirel YA, Fudala R, et al. Structure of the O-polysaccharide of Proteus mirabilis O38 containing 2-acetamidoethyl phosphate and N-linked D-aspartic acid. Carbohydr Res. 2003 Oct 31;338(22):2387–2392.

. Bandyopadhyay PK, Nayak AK. Thiolation Of Fenugreek Seed Polysaccharide; Utilization As A Novel Biomucoadhesive Agent In Drug Delivery. Int J Appl Pharm. 2023 Jan 7;15(1):290–297.

. Qian S, Fang X, Dan D, Diao E, Lu Z. Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity. RSC Adv. 2018;8(73):42145–42152.

. Badrinathan S, Shiju TM, Sharon Christa AS, Arya R, Pragasam V. Purification and Structural Characterization of Sulfated Polysaccharide from Sargassum myriocystum and its Efficacy in Scavenging Free Radicals. Indian J Pharm Sci. 2012;74(6):549–555.

. Kołodziejczak-Radzimska A, Bielejewski M, Biadasz A, Jesionowski T. Evaluation of MxOy/fucoidan hybrid system and their application in lipase immobilization process. Sci Rep. 2022 May 4;12(7218):1-14.

. Olasehinde TA, Mabinya LV, Olaniran AO, Okoh AI. Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities. Int J Food Prop. 2019 Jan 1;22(1):100–10.

. Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic Assay. Appl Microbiol. 1971 Oct;22(4):659–665.

. Annas ZF, Muliasari H, Deccati RF, Permatasari L, Mukhlishah NRI. Determination of total flavonoid content of extract and fractions of mangrove leaves (Avicennia marina). J Agrotek Ummat. 2023 Aug 25;10(3):271–282.