ANTI-INFLAMMATORY ACTIVITY AND IRRITATION TEST OF TOPICAL DOSAGE FORMS OF CLOVE LEAF ESSENTIAL OIL (SYZYGIUM AROMATICUM) IN ANIMALS

IKA BUANA JANUARTI; FADZIL LATIFAH; RICKY FIRMANSYAH; AULYA D. PERTIWI; NIKEN AGUSTYA; HESTI WIDYAWATI

doi:10.22159/ijap.2024v16s5.52483

Authors

IKA BUANA JANUARTI Program Study of Pharmacy, Faculty of Pharmacy, Universitas Islam Sultan Agung, Jl. Raya Kaligawe KM.4, Semarang-50112, Indonesia
FADZIL LATIFAH Program Study of Pharmacy, Faculty of Pharmacy, Universitas Islam Sultan Agung, Jl. Raya Kaligawe KM.4, Semarang-50112, Indonesia
RICKY FIRMANSYAH Program Study of Pharmacy, Faculty of Pharmacy, Universitas Islam Sultan Agung, Jl. Raya Kaligawe KM.4, Semarang-50112, Indonesia
AULYA D. PERTIWI Program Study of Pharmacy, Faculty of Pharmacy, Universitas Islam Sultan Agung, Jl. Raya Kaligawe KM.4, Semarang-50112, Indonesia
NIKEN AGUSTYA Program Study of Pharmacy, Faculty of Pharmacy, Universitas Islam Sultan Agung, Jl. Raya Kaligawe KM.4, Semarang-50112, Indonesia
HESTI WIDYAWATI Program Study of Pharmacy, Faculty of Pharmacy, Universitas Islam Sultan Agung, Jl. Raya Kaligawe KM.4, Semarang-50112, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16s5.52483

Keywords:

Eugenol, Essential oils, Anti-inflammatory, Irritation test, Clove leaf, Emulgel, Stick balm, Balsam, Massage oil

Abstract

Objective: Clove leaves (Syzygium aromaticum) contain compounds namely eugenol, alpha-humulene, beta-caryophyllene, and caryophyllene oxide with the main compound eugenol which has anti-inflammatory activity. One of dosage form to treat inflammation on the skin is a topical dosage form because it is quickly absorbed into the skin. This study aimed to formulate Clove Leaf Essential Oil (CLEO) and evaluate the physical properties, anti-inflammatory activity, and irritation effects of various topical dosage forms (emulgel, balsam, massage oil, and stick balm).

Methods: Research design was a pre-posttest control group. Essential oils were obtained using the steam distillation method. The four different concentrations of CLEO topical dosage form (emulgel, balsam, massage oil, and stick balm) are 5% and 10%. Then, test are conducted on the physical qualities (organoleptic, pH, displaced volume, spreadability, sticking power, moisture content and viscosity). Anti-inflammatory activity using the carrageenan-induced paw edema method in Wistar rats was divided into four groups are dosage form CLEO concentrations of 5% and 10%, base preparation, and positive control. Anti-inflammatory activity results were analyzed with Post-hoc statistical analysis with a 95% confidence level. The irritation method was used to measure the Primary Irritation Index (PII) followed procedure by Indonesian Food and Drug Authority about Guidelines for In Vivo Preclinical Toxicity Testing.

Results: All these dosage forms met the physical requirements. Anti-inflammatory activity, will rise as result of increasing the CLEO content in the formulae. All these dosage forms did not cause irritation in animal.

Conclusion: Among the four formulations, balsam CLEO 10% showed maximum inhibitory oedema (94.05%), at the end of 360 minutes followed by balsam CLEO 5%, emulgel CLEO 10%, stick balm CLEO 10% and massage oil CLEO 10%

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References

O. Dhyantari, C. T. Milala, and T. D. Widyaningsih, “Efek Antiinflamasi Dari Ekstrak Glukosamin Ceker Ayam Pada Tikus Wistar Jantan Yang Diinduksi Karagenan,” vol. 3, no. 3, 2015.

Katzung and B. G., Farmakologi Dasar dan Klinik Ed 6. Jakarta: EGC, 2006.

Sumiyati Y., Nafisa S., Wiwi W., Mumpuni, E., Pratami, D.K., Aulena, D.N., Yantih, N. and Joti. “Formulation And Evaluation Of Red Ginger Oil (Zingiber officinale roscoe) Balm As An Analgesic,” Int. J. Appl. Pharm., pp. 88–90, Jun. 2022, doi: 10.22159/ijap.2022.v14s3.18.

Tendeng, F. C. A., Longdong, I. A. and Tooy, D. “Uji Teknis Alat Pembuatan Minyak Daun Cengkeh Untuk Industri Kecil". Cocos, vol. 6, no. 6, 2021, doi: https://doi.org/10.35791/cocos.v6i6.35576.

Wibowo, D. P., Rustamsyah, A. and Kurniawan, Y., “Karakterisasi Dan Aktivitas Repelen Minyak Atsiri Sereh Wangi (Cymbopogon nardus L), Akar Wangi (Vetiveria zizanoides L.), Nilam (Pogestemon Cablin), Cengkeh (Syzgium aromaticum) Asal Kabupaten Garut Terhadap Nyamuk Aedes aegypti BETINA,” J. Ilmu Farm. Dan Farm. Klin., vol. 13, no. 2, pp. 1–6, 2016, doi: http://dx.doi.org/10.31942/jiffk.v13i2.1702.

Malahayati M. and Rahmawati, “Optimasi tekanan dan rasio refluks pada distilasi fraksinasi vakum terhadap mutu Eugenol dari Minyak Daun Cengkeh (Eugenia caryophyllata),” Konversi, vol. 3, no. 2, pp. 7–17, 2014.

R. Irwanto, A. Kasim, and S. D. Ismanto, “Penentuan Kadar Minyak Atsiri Daun Kayu Manis (Cinnamomum burmannii, Blume) dengan Perlakuan Pendahuluan pada Daun,” J. Teknol. Pengolah. Pertan., vol. 4, no. 1, p. 1, Jul. 2022, doi: 10.35308/jtpp.v4i1.5661.

M. F. Kurniawan, N. Sugihartini, and T. Yuwono, “Uji Iritasi Serta Evaluasi Sifat Fisik Emulgel Minyak Atsiri Bunga Cengkeh Dengan Metode Simplex Latice Design.,” Farmasains, vol. 5, no. 1, 2018.

S. Jamal and Y. Anwar, “Uji Аktivitаs Аntiinflаmаsi Minyаk Gosok Minyаk Аtsiri Serаi Wаngi (Cympobogon nаrdus) Terhаdаp Tikus Putih Jаntаn (Rаttus norwegicus) Аnti-Inflаmmаtory,” Pharm. J., vol. 3, no. 2, pp. 69–82, 2019.

M. S. Komang, T. N. L. Putu, and A. I. Nengah, “Studi Pengaruh Lamanya Pemaparan Medan Magnet Terhadap Jumlah Sel Darah Putih (Leukosit) Pada Tikus Putih (Rattus norwegicus),” Bul. Fis., vol. 15, no. 1, pp. 31–38, 2014.

BPOM RI, Peraturan Badan Pengawas Obat dan Makanan Nomor 25 Tahun 2019 Tentang Cara Pembuatan Kosmetika Yang Baik. Jilid I Peraturan BPOM Tahun 2019. 2019.

United States, The United States Pharmacopeia NF 38, vol. 1. 2020.

Kementrian kesehatan RI, Farmakope Indonesia Edisi VI. Kementrian Kesehatan RI., 2020.

S. Doppalapudi, V. Suryadevara, S. K. Ainampudi, S. L. Reddyvallam, and R. Anne, “Formulation and evaluation of Anti-inflammatory activity of Lemon grass oil Liniments on wistar rats,” Asian J. Pharm. Pharmacol., vol. 4, no. 4, pp. 434–439, Aug. 2018, doi: 10.31024/ajpp.2018.4.4.9.

S. A. Istiqomah, Nofita, and Hidayaturahmah, “Perbandingan Aktivitas Antiinflamasi Ekstrak Daun Kemangi (Ocimun basilicum L.) dan Ekstrak Daun Cincau Hijau (Cyclea barbata Miers.).,” Malahayati, 2023. [Online]. Available: https://doi.org/10.5281/zenodo.7549331

BPOM RI, “Peraturan Badan Pengawas Obat Dan Makanan Nomor 10 Tahun 2022 Tentang Pedoman Uji Toksisitas Praklinik Secara In Vivo.,” 2022.

L. Pratiwi and M. S. Rachman, “Ektraksi Minyak Atsiri Dari Bunga Cengkeh Dengan Pelarut Etanol Dan N-Heksana,” 3rd Universty Res. Coloquium 2016, pp. 131–137, 2016.

Nirwana C. H. and Zamrudy W., “Studi Literatur Karakteristik Minyak Cengkeh (Clove Oil) Dari Beberapa Metode Distilasi,” Distilat J. Teknol. Separasi, vol. 7, no. 2, pp. 561–569, 2021, doi: https://doi.org/10.33795/distilat.v7i2.271.

Dharmawan, I. B. N. Y. “Isolasi Dan Analisis Gc/Ms Minyak Cengkeh Dari Bunga Cengkeh (Syzigium aromaticum),” pp. 1–8, Jan. 2016.

S. Rohmani et al., “Characteristics Of Eugenol Products And In Vitro Release In Gel Base With Hydroxypropyl Methylcellulose (HPMC) Variant As Gelling Agent,” Int. J. Appl. Pharm., pp. 106–111, Nov. 2021, doi: 10.22159/ijap.2021v13i6.42351.

F. A. Iriani, N. Sugihartini, and T. Yuwono, “The Profile of Anti-inflammatory Activity of Syzigium Aromaticum Volatile Oil in Lotion with Variation Composition of Oleic Acid and Propylene Glycol as Enhancer,” Majalah Obat Tradisional., vol. 22, no. 2, p. 111, Aug. 2017, doi: 10.22146/tradmedj.27922.

F. B. P. Paputungan, “Uji Efektivitas Antiinflamasi Salep Ekstrak Buah Cengkeh (Syzygium aromaticum L) Pada Tikus Putih (Rattus norvegicus),” PHARMACON, vol. 11, no. 2, pp. 1473–1480, Mei 2022.

Ritmaleni, Juson, A. K. Zulkarnain, A. E. Indaryanti, and A. Fatmawati, “The Effect Of Variation Concentration Of Tetrahydrohexagamavunon-5 (THHGV-5) In Emulgel Preparation On Acute Dermal Irritation Effect And Sun Protecting Factor (SPF) Value,” Int. J. Appl. Pharm., pp. 282–287, Nov. 2023, doi: 10.22159/ijap.2023v15i6.47741.

D. Rahmi et al., “Antioxidant Activity, Skin Irritation Potential And Chemical Composition Of Clove Leaf Oil From West Java Indonesia,” J. Sains Materi Indones., vol. 23, no. 1, p. 24, Nov. 2021, doi: 10.17146/jsmi.2021.23.1.6398